Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6

Abstract

The Gram-positive, alkali- and salt-tolerant marine bacterium strain P203T is described together with its closest phylogenetic neighbour, terrestrial isolate LMG 21005T. Strain P203T was isolated from material from the sponge Plakortis simplex that was obtained from the Sula-Ridge, Norwegian Sea. Strain LMG 21005T was an undescribed strain that was isolated from a church wall mural in Germany. Strains P203T and LMG 21005T were identified as novel alkalitolerant members of the Bacillus rRNA group 6 with a 16S rRNA gene sequence similarity of 99.5 %. The closest described neighbour, Bacillus gibsonii DSM 8722T, showed 99.0 % gene sequence similarity with P203T and 98.8 % similarity with strain LMG 21005T. Despite the high 16S rRNA gene sequence similarity, DNA–DNA cross-hybridization revealed only 25.8–34.1 % similarity amongst the three strains. The DNA G+C contents were 41.1 mol% for strain P203T and 39.6 mol% for strain LMG 21005T. Both strains grew well between pH 7 and pH 11. Strain P203T showed growth at moderate temperatures (from 4 to 30 °C) and in the presence of up to 12 % (w/v) NaCl at pH 9.7, whereas strain LMG 21005T was not salt tolerant (up to 4 % NaCl) and no growth was observed at 4 °C. The major fatty acids of strains P203T, LMG 21005T and the type strain of B. gibsonii were the saturated terminally methyl-branched compounds iso-C15 : 0 (19.8, 15.6 and 28.0 %, respectively) and anteiso-C15 : 0 (57.1, 48.6 and 45.2 %, respectively). Physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains P203T and LMG 21005T from the six related Bacillus species with validly published names and supported the proposal of two novel species, Bacillus plakortidis [type strain P203T (=DSM 19153T=NCIMB 14288T)] and Bacillus murimartini [type strain LMG 21005T (=NCIMB 14102T)].

Microbial communities associated with the anthropogenic, highly alkaline environment of a saline soda lime, Poland

“…A73, first isolated from a hypersaline lake in Iran (Amoozegar et al 2014). Strain MI12 was identified as alkalitolerant Bacillus plakortidis , which was first isolated from a sponge in the Norwegian Sea (Borchert et al 2007). The closest relative of isolate MI17 was Bacillus sp.…”

Analysis of the effect of high hydrostatic pressure treatment and enterocin AS-48 addition on the bacterial communities of cherimoya pulp

“…Species of genus Bacillus are known for their capacity to produce an array of antimicrobial substances and extracellular enzymes, which may altogether facilitate substrate utilization and displacement of competitors. Interestingly, among the main OTUs detected for endosporeformers were B. firmus and Bacillus plakortidis, both of them alkali-tolerant bacteria that have also been isolated from seawater environments (Borchert et al, 2007;Geng et al, 2014). The tropical coast of Southern Spain is known for the intensive farming of cherimoya trees.…”

Draft Genome Sequence of Bacillus murimartini LMG 21005 T , an Alkalitolerant Bacterium Isolated from a Church Wall Mural in Germany

“…The type strain LMG 21005 T of Bacillus murimartini was isolated from a church wall mural in Germany and identified as a novel alkalitolerant member of the Bacillus rRNA group 6 ( 1 ). B. murimartini LMG 21005 T showed 98.8% similarity with the closest described neighbor, Bacillus gibsonii DSM 8722 T ( 2 ).…”

“…B. murimartini LMG 21005 T showed 98.8% similarity with the closest described neighbor, Bacillus gibsonii DSM 8722 T ( 2 ). The optimal growth of B. murimartini LMG 21005 T was achieved at 15°C to 20°C and pH 8.5; it was not salt tolerant (up to 4% NaCl) ( 1 ). Notably, there is no other information about B. murimartini except its taxonomical description so far.…”