Bacillus phytases: Current status and future prospects

Borgi, Mohamed Ali; Boudebbouze, Samira; Mkaouar, Héla; Maguin, Emmanuelle; Rhimi, Moez

doi:10.1080/21655979.2015.1048050

Cited by 17 publications

(9 citation statements)

References 45 publications

(43 reference statements)

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“…A considerable amount of soil phosphorus is also found in the form of phytate (inositol phosphate) which is a complex of the phosphorus with other minerals (Findenegg and Nelemans 1993). Most of the Bacillus species identi ed in this study have been reported to produce phytase, an enzyme which cleaves phytate into soluble form (Borgi et al 2015). The abundance and e cacy of phytase producing microbes has been already assessed in tea garden soils of NE India (Pramanik et al 2014).…”

Section: Discussionmentioning

confidence: 76%

Niche differentiation of microbes and their functional signatures in Assam type tea (Camellia sinensis var. assamica)

Bora¹,

Dey²,

Borah³

et al. 2021

Preprint

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We employed an Illumina-based high throughput metagenomics sequencing approach to unveil the overall rhizospheric as well as endophytic microbial community associated with an organically grown Camellia population located at the Experimental Tea Garden, Assam Agricultural University, Assam (India). Quality control (i.e. adapter trimming and duplicate removal) followed by de novo assembly revealed the tea endophytic metagenome to contain 24,231 contigs (total 7,771,089 base pairs with an average length of 321 bps) while tea rhizospheric soil metagenome contained 261,965 sequences (total 230537174 base pairs, average length 846). The most prominent rhizobacteria belonged to the genus viz., Bacillus (10.34%), Candidatus Koribacter (8.0%), Candidatus Solibacter (6.35%), Burkholderia (5.18%), Acidobacterium (4.08%), Pseudomonas (3.9%), Streptomyces (3.52%), Bradyrhizobium (2.76%) and Enterobacter (2.56%); while the endosphere was dominated by bacterial genus viz., Serratia (42.3%), Methylobacterium (7.6%), Yersinia (5.4%), Burkholderia (2.2%) etc. The presence of few agronomically important bacterial genuses such as Bradyrhizobium (1.18%), Rhizobium (0.8%), Sinorhizobium (0.34%), Azorhizobium and Flavobacterium (0.17% each) were also detected in the endosphere. KEGG pathway mapping highlighted the presence of microbial metabolite pathway genes related to tyrosine metabolism, tryptophan metabolism, glyoxylate and dicarboxylate metabolism and amino sugar metabolism which play important roles in endophytic activities including survival, growth promotion and host adaptation.

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Section: Discussionmentioning

confidence: 76%

Niche differentiation of microbes and their functional signatures in Assam type tea (Camellia sinensis var. assamica)

Bora¹,

Dey²,

Borah³

et al. 2021

Preprint

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“…These host genes are capable of metabolizing imino and amino acids [ 50 ]. These strain properties have been widely studied and applied to various bio-chemical industrial fields as a detergent agent [ 52 ], α-amylase [ 58 , 59 ], peptide antibiotics [ 60 , 61 ], fungal pathogens inhibitors [ 62 ], β-mannanase [ 63 , 64 ], cycloglucosyltransferase [ 65 ], pectinase [ 66 , 67 ], pentosanase [ 68 ], and phytase [ 54 , 69 , 70 ]. Although several B. licheniformis studies have contributed to better understanding of the strain, the following aspects of the enzyme have yet to be examined: the cloning of B. licheniformis cellulase genes, its E. coli cell-surface display, and numerous enzymatic characteristics.…”

Section: Resultsmentioning

confidence: 99%

Bacillus Cellulase Molecular Cloning, Expression, and Surface Display on the Outer Membrane of Escherichia coli

Kim

2018

Molecules

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One of the main challenges of using recombinant enzymes is that they are derived from genetically-modified microorganisms commonly located in the intracellular region. The use of these recombinant enzymes for commercial purposes requires the additional processes of cell disruption and purification, which may result in enzyme loss, denaturation, and increased total production cost. In this study, the cellulase gene of Bacillus licheniformis ATCC 14580 was cloned, over-expressed, and surface displayed in recombinant Escherichia coli using an ice-nucleation protein (INP). INP, an outer membrane-bound protein from Pseudomonas syringae, was utilized as an anchor linker, which was cloned with a foreign cellulase gene into the pET21a vector to develop a surface display system on the outer membrane of E. coli. The resulting strain successfully revealed cellulase on the host cell surface. The over-expressed INP-cellulase fusion protein was confirmed via staining assay for determining the extracellular cellulase and Western blotting method for the molecular weight (MW) of cellulase, which was estimated to be around 61.7 kDa. Cell fractionation and localization tests demonstrated that the INP-cellulase fusion protein was mostly present in the supernatant (47.5%) and outer membrane (19.4%), while the wild-type strain intracellularly retained enzymes within cytosol (>61%), indicating that the INP gene directed the cellulase expression on the bacteria cell surface. Further studies of the optimal enzyme activity were observed at 60 °C and pH 7.0, and at least 75% of maximal enzyme activity was preserved at 70 °C.

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“…The futuristic approach will develop economic and novel bio‐engineered phytase enzymes for simultaneous phytic acid degradation. Acid phosphatase/phytase 2 (appA2) from Escherichia coli , PhyL phytase from Bacillus licheniformis ATCC 14580, phytase from Weissella halotolerans are some examples of bio‐engineered phytase enzymes found to be effective against phytic acid (Borgi, Boudebbouze, Mkaouar, Maguin, & Rhimi, 2015; Demir, Şenol Kotan, Dikbaş, & Beydemir, 2017; Pakbaten et al, 2019). However, it requires human trials and prior approval by several health organizations for commercial use.…”

Section: Economic Aspects and Future Directionsmentioning

confidence: 99%

“…are some examples of bio-engineered phytase enzymes found to be effective against phytic acid (Borgi, Boudebbouze, Mkaouar, Maguin, & Rhimi, 2015;Demir, S ¸enol Kotan, Dikbas ¸, & Beydemir, 2017;Pakbaten et al, 2019). However, it requires human trials and prior approval by several health organizations for commercial use.…”

Section: Economic Aspects and Future Directionsmentioning

confidence: 99%

Phytic acid and its reduction in pulse matrix: Structure–function relationship owing to bioavailability enhancement of micronutrients

Sarkhel

Roy

2022

J Food Process Engineering

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The bioavailability of minerals and proteins in the pulse is endured due to the presence of antinutrient phytic acid. Phytic acid is a proton donor that produces hydrogen ions (H + ) and "phytate" anion, which readily forms salts with available minerals and protein. These phytate complexes are not bioavailable to humans and led to several public health problems like anemia, skeletal abnormalities, osteoporosis, malabsorption, and kwashiorkor syndrome due to Fe, Mn, Ca, Zn, and protein deficiency. Thus, it is a severe concern to the vegetarian population who strongly rely upon pulses to fulfill their daily nutrition requirements. Improving the bioavailability of micronutrients in the pulse matrix requires reduction of phytic acid. A phytic acid reduction strategy should ensure its efficient reduction leading to enhanced micronutrient bioavailability. This article provides a throughout view of the available phytic acid reduction technologies conducted at the postharvest stages of pulse processing.The mechanism of phytic acid reduction during conventional (soaking, cooking, and germination) and novel processing (irradiation, ultrasound, and high pressure) regarding its degradation and interaction with other components has been explored here, providing insight into structure-function relationships. Further, an in-depth analysis of the combined treatments offering better phytic acid reductions has been explained in the light of structural alteration of phytic acid to its lower inositol phosphates. The summarized data on prospects and drawbacks of different phytic acid reduction strategies would provide background information to explore the futuristic possibilities for efficient phytic acid reduction and micronutrient fortification. Practical ApplicationsThe presence of phytic acid in the pulse matrix restricts the micronutrient absorption by complexing with micronutrients leading to their ineffectiveness. The bottom-line population of the income pyramid, who are unable to intake micronutrients from other sources, suffers from several public health problems related to nutrient or combined nutrient deficiencies. Therefore, the degradation of phytic acid in pulses is necessary. This article aims to provide a comprehensive overview and explanation of phytic acid degradation during various postharvest pulses processing. The chelation

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Bacillus phytases: Current status and future prospects

Cited by 17 publications

References 45 publications

Niche differentiation of microbes and their functional signatures in Assam type tea (Camellia sinensis var. assamica)

Niche differentiation of microbes and their functional signatures in Assam type tea (Camellia sinensis var. assamica)

Bacillus Cellulase Molecular Cloning, Expression, and Surface Display on the Outer Membrane of Escherichia coli

Phytic acid and its reduction in pulse matrix: Structure–function relationship owing to bioavailability enhancement of micronutrients

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