Bacillus methylotrophicus has potential applications against Monilinia fructicola

Abstract

Biocontrol is a cost-effective and environmentally friendly technique used in agricultural production. We isolated and screened a bacterial strain from the soils of a peach orchard with high yield. Using biochemical and physiological analysis as well as phylogenetic sequencing data, we identified a strain of Bacillus methylotrophicus, strain XJ-C. The results of our screening trials showed that XJ-C was able to suppress M. fructicola at an inhibition rate of 81.57%. Following the application of a 1×109 CFU/mL XJ-C strain suspension to the fruits, leaves, and shoots of peach trees infected with M. fructicola, the inhibition rate reached 64.31%, 97.34%, and 64.28%, respectively. Using OM and SEM, we observed that, under the inhibition of strain XJ-C, M. fructicola mycelium and spores were abnormally shaped. Under TEM, cell walls were transparent, organelles had disappeared, and the intracellular vacuole was deformed. Thus, XJ-C has the potential to be used in biocontrol.

Purification and identification of a new antifungal dipeptide from Bacillus velezensis AR1 culture supernatant

“…goeosporioides and M. fructicola fungal pathogens that cause anthracnosis and brown rot on peach fruits, respectively. Recent reports indicated that Pseudomonas synxantha strongly reduced the mycelial growth of M. fructicola grown on peach extract agar 18 ; and inhibited the growth of the pathogen on fruit, leaf and shoots by 64.3%, 97.3% and 64.3%, respectively 19 . Likewise, two Bacillus species, namely, P. polymyxa APEC136 and B. subtilis APEC170 also were reported as strong inhibitors of the apple anthracnosis causal pathogen, C .…”

Study on the optimal antagonistic effect of a bacterial complex against Monilinia fructicola in peach

“…The antagonistic effect of the bacterial complex on M. fructicola was determined as described in ref. [ 21 ]. The fermentation broth of complex bacteria was studied using three gradients, i.e., 1 × 10 7 , 1 × 10 8 , and 1 × 10 9 CFU/mL.…”

“…Three strains of B. subtilis (Ehrenberg) Cohn (xj-14, xj-15, and xj-16), one strain of B. tequilensis Gatson (xj-A), and one strain of B. methylotrophicus (xj-C) with strong antagonistic activity toward peach brown rot were isolated in our previous research [ 20 , 21 ]. However, each individual strain was effectively antagonistic only for a short time.…”