Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India

The present study reports two bacteria, designated 87I and 112A, which were isolated from soil and activated sludge samples from Hyderabad, India, and that are capable of producing poly-3-hydroxybutyrate (PHB). Based on phenotypical features and genotypic investigations, these microorganisms were identified as Bacillus spp. Their optimal growth occurred between 28 degrees C and 30 degrees C and pH 7. Bacillus sp. 87I yielded a maximum of 70.04% dry cell weight (DCW) PHB in medium containing glucose as carbon source, followed by 55.5% DCW PHB in lactose-containing medium, whereas Bacillus sp. 112A produced a maximum of 67.73% PHB from glucose, 58.5% PHB from sucrose, followed by 50.5% PHB from starch as carbon substrates. The viscosity average molecular mass (M (v)) of the polymers from Bacillus sp. 87I was 513 kDa and from Bacillus sp. 112A was 521 kDa. All the properties of the biopolymers produced by the two strains 87I and 112A were characterized.

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“…87I and Bacillus sp. 112A with their closely related B. cereus and B. Xexus, respectively a Data from Matsumoto et al[20] and Suresh et al[21] …”

mentioning

confidence: 97%

Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge

Thirumala

Reddy

Mahmood

2009

J Ind Microbiol Biotechnol

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“…Therefore a "green", non-toxic way of synthesising metallic nanoparticles is needed in order to allow them to be used in a wider range of industries. This could potentially be achieved by using biological methods.Many bacteria, fungi and plants have shown the ability to synthesise metallic nanoparticles and all have their own advantages and disadvantages (Table 1) [16][17][18]. Intracellular or extracellular synthesis, growth temperature, synthesis time, ease of extraction and percentage synthesised versus percentage removed from sample ratio, all play an important role in biological nanoparticle production.…”

mentioning

confidence: 99%

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Pantidos¹

2014

J Nanomed Nanotechnol

441

221

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Over the past few decades interest in metallic nanoparticles and their synthesis has greatly increased. This has resulted in the development of numerous ways of producing metallic nanoparticles using chemical and physical methods. However, drawbacks such as the involvement of toxic chemicals and the high-energy requirements of production make it difficult for them to be widely implemented. An alternative way of synthesising metallic nanoparticles is by using living organisms such as bacteria, fungi and plants. This "green" method of biological nanoparticle production is a promising approach that allows synthesis in aqueous conditions, with low energy requirements and low-costs. This review gives an overview of some of these environmentally friendly methods of biological metallic nanoparticle synthesis. It also highlights the potential importance of these methods in assessing nanoparticle risk to both health and the environment.techniques, which are generally low-cost and high-volume. However, the need for toxic solvents and the contamination from chemicals used in nanoparticle production limit their potential use in biomedical applications [15]. Therefore a "green", non-toxic way of synthesising metallic nanoparticles is needed in order to allow them to be used in a wider range of industries. This could potentially be achieved by using biological methods.Many bacteria, fungi and plants have shown the ability to synthesise metallic nanoparticles and all have their own advantages and disadvantages (Table 1) [16][17][18]. Intracellular or extracellular synthesis, growth temperature, synthesis time, ease of extraction and percentage synthesised versus percentage removed from sample ratio, all play an important role in biological nanoparticle production. Finding the right biological method can depend upon a number of variables. Most importantly, the type of metal nanoparticle under investigation is of vital consideration, as in general organisms have developed resistance against a small number of metals, potentially limiting the choice of organism. However synthetic biology; a nascent field of science, is starting to address these issues in order to create more generalised chassis, able to synthesise more than one type of metallic nanoparticle using the same organism [19]."Natural" biogenic metallic nanoparticle synthesis can be split into two categories. The first is bioreduction, in which metal ions are chemically reduced into more stable forms biologically. Many organisms have the ability to utilise dissimilatory metal reduction, in which the reduction of a metal ion is coupled with the oxidation of an enzyme [20]. This results in stable and inert metallic nanoparticles that can then be safely removed from a contaminated sample. The second category is biosorption. This involves the binding of metal ions from Journal of Nanomedicine & Nanotechnology J o u rna l of N a n o m ed icine & N a n o te chnolo g y

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“…Bacillus thioparans NR_043762 The B. indicus was first discovered in fresh water polluted by arsenic in Bengla basin in India and showed resistance to toxic heavy metals (Suresh et al 2004). Subsequently, it was also isolated from seawater (Nithya and Pandian 2010) and safely used as a food additive for pigs and rabbits (Hong et al 2008).…”

Section: Bacillus Herbersteinensisnr_042286mentioning

confidence: 99%

Screening and identification of organics-degrading bacteria from the sediment of sea cucumber Apostichopus japonicus ponds

Zhang

Liu

et al. 2015

Aquacult Int

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The organics-degrading bacteria were isolated from sea cucumber (Apostichopus japonicus) outdoor rearing ponds sediment. The bacterial strains were selected according to the isolation growth, pathogenicity, organic degradation rate, and effects on A. japonicus growth and water quality. The screened strains were identified on physiological, biochemical, and genetic characteristics. Among 68 strains isolated, two strains (L15 and E4) were identified as potential organics-degrading probiotics. The degradation rates on sediment chemical oxygen demand (COD) were 46.95 ± 1.58 % for the L15 strain and 44.31 ± 1.44 % for the E4 strain. The L15 strain enhanced the specific growth rate (SGR) of A. japonicus by 1.97 ± 0.49 %, and the E4 strain enhanced SGR by 2.24 ± 0.44 % compared with the SGR of 0.55 ± 0.41 % in the control group without probiotics. The COD level increased by -0.9 ± 1.42 % of L15 and 47.9 ± 0.06 % of E4 inoculations after 7 days, while the COD increased by 140.6 ± 4.22 % in the control. Based on the analyses of biochemical characteristics and 16S rRNA gene sequences, the L15 and E4 strains were identified as Bacillus spp. and Pseudomonas spp., respectively.

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Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India

Cited by 101 publications

References 40 publications

Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge

Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge

Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants

Screening and identification of organics-degrading bacteria from the sediment of sea cucumber Apostichopus japonicus ponds

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