Bacillus hwajinpoensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea

Abstract

Two Gram-positive or -variable, endospore-forming, slightly halophilic strains (SW-72T and SW-93) were isolated from sea water of the East Sea and the Yellow Sea in Korea, respectively, and subjected to polyphasic taxonomic study. Both strains had cell-wall peptidoglycan that was based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The two strains contained large amounts of saturated and branched fatty acids, with anteiso-C15 : 0 as the major fatty acid. The DNA G+C contents of strains SW-72T and SW-93 were 40·9 and 41·0 mol%, respectively. Phylogenetic analysis based on 16S rDNA sequences showed that strains SW-72T and SW-93 fall within the radiation of the cluster that comprises members of the genus Bacillus, particularly Bacillus rRNA group 6. There were five nucleotide differences between the 16S rDNA sequences of strains SW-72T and SW-93. The mean level of DNA–DNA relatedness between strains SW-72T and SW-93 was 21·5 %. Strains SW-72T and SW-93 showed 93·1–95·2 % 16S rDNA sequence similarity to the type strains of Bacillus species that are assigned to rRNA group 6. Strains SW-72T and SW-93 could not be differentiated clearly by using their phenotypic properties. On the basis of phenotypic properties, phylogeny and genomic data, it is proposed that strain SW-72T (=KCCM 41641T=JCM 11807T) should be placed in the genus Bacillus as the type strain of a novel species, Bacillus hwajinpoensis sp. nov., and that strain SW-93 (=KCCM 41640=JCM 11806) should be placed in the genus Bacillus as an unnamed Bacillus genomospecies.

Diversity and bioactive potential of endospore-forming bacteria cultured from the marine sponge Haliclona simulans

“…baekryungensis isolates were unable to grow, or grew poorly, at 40°C (Table 3). This contrasted to what has been reported for the type strains of these species (Yoon et al. 2004).…”

“…2). This included recently described species that have been primarily or exclusively reported from marine habitats including B. aquimaris , B. algicola , B. baekryungensis and B. hwajinpoensis (Yoon et al. 2003, 2004; Ivanova et al.…”

Specific rhizobacterial resources: characterization and comparative analysis from contrasting coastal environments of Korea

“…According to previous studies on type strains in phylogenetic trees, most were isolated from marine or highly saline environments [8,[21][22][23][24][25][26][27]. However, our sampling was performed one week after typhoon extinction, and no severe waves or sea fog were reported.…”

Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius

“…Both strains were negative for the following: production of indole and acetoin; hydrolysis of agar, starch, urea, Tweens 20, 40 and 80 and chitin; acid production from L-arabinose, melibiose, L-rhamnose, D-xylose, adonitol, dulcitol, inositol, sorbitol and citrate; and utilization of L-arabinose, D-lactose, inositol and sorbitol a Data from Heyrman et al (2003) and this study b E Ellipsoidal, S Spherical, C Central, ST Subterminal c ? positive reaction; -negative reaction relatives of the genus Bacillus (Heyrman et al 2003;Ivanova et al 2004;Lim et al 2006;Yoon et al 2004). However, the novel strain can clearly be distinguished from its nearest neighbor, B. decolorationis LMG 19507 T , on the basis of some differences in the polar lipid and fatty acid composition ( Fig.…”

“…The majority of members of the genus were isolated from soil samples but the type strains of only thirteen species, including B. algicola, B. alveayuensis, B. aquimaris, B. chungangensis, B. coahuilensis, B. galliciensis, B. hwajinpoensis, B. litoralis, B. marisflavi, B. neizhouensis, B. oceanisediminis, B. plakortidis and B. qingdaonensis, have so far been recovered from different marine environments (Bae et al 2005;Balcázar et al 2010;Borchert et al 2007;Cerritos et al 2008;Chen et al 2009;Cho et al 2010;Ivanova et al 2004;Wang et al 2007;Yoon et al 2003Yoon et al , 2004Yoon et al , 2005Zhang et al 2010). It is interesting that all reported marine Bacillus species are characterized by an aerobic type of metabolism.…”