Bacillus cereus and Bacillus subtilis used as probiotics in rotifer (Brachionus plicatilis) cultures

doi:10.4172/2155-9546.s1-007

Cited by 13 publications

(10 citation statements)

References 45 publications

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“…Furthermore, spores of Bacillus species when fed to rotifer (B. plicatilis) lead to improve the bacterial load from aquatic environment as well as dietary utilization of larvae in turbot (S. maximus; Gatesoupe 1989). Murillo and Villamil (2011) also demonstrated significant increase in rotifer counts by addition of B. subtilis to the rotifer culture. Similarly, Motlagh et al (2012) recorded improved growth, gut microbiota and digestive enzymatic (protease and amylase) activities in A. urmiana fed with 10 4 CFU g À1 B. subtilis and B. licheniformis.…”

Section: As a Probiotic In Synbioticsmentioning

confidence: 84%

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Nayak

2020

Reviews in Aquaculture

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Microorganisms play an important role in water quality maintenance, waste management, productivity enhancement, nutrient cycling, functional feed development, protection against infectious diseases, delivery of antigens and several other health benefits in aquaculture. The most significant use of microbes in aquaculture to minimize the antibiotics and drugs is through probiotics. The scope of probiotics in modern aquaculture has been enormous due to the detrimental consequences of routinely used antibiotics and chemotherapeutic agents and demand for safe aquatic products for human consumption. Since past few decades, both scientific and commercial interests are burgeoning in this field, and over the years, several probiotics are successfully used in aquaculture. Among various probiotics that are being used, Bacillus species are the common probiotics that are used in different aquatic animals. Several probiotics species belonging to Bacillus genus are currently been used, and Bacillus subtilis is one of such probiotics that is very well‐researched and most widely used probiotics in aquaculture. While its probiotic use has already been an important component in diverse aquaculture practices, its application in water quality improvement, utility in bioflocs/bioremediation and vehicle to express and delivery of heterologous antigen are noteworthy. It exerts innumerable beneficial effects such as improvement of growth, nutrition, immunity and disease resistance in aquatic species. This bacterium adopts a variety of disparate and overlapping mechanisms such as synergistic, antagonistic, competitive exclusion and immune‐stimulating effects. This review summarizes various applications of this bacterium as probiotics, bioremediating agent, bioflocing agent and possible live vaccine vehicle in aquaculture.

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Section: As a Probiotic In Synbioticsmentioning

confidence: 84%

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Nayak

2020

Reviews in Aquaculture

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“…LAB is recognized as a part of the microflora of rotifers (Gatesoupe, 1994), but its specific role is not yet fully understood. Applied to zooplankton cultures, B. subtilis acts as a growth promoter (Hirata et al, 1998;Murillo & Villamil, 2011;Zink et al, 2013); as a digestive enzyme promoter (Motlagh, Farhangi, Rafiee, & Noori, 2012); and as a pathogen inhibitor (Giarma et al, 2017;Murillo & Villamil, 2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of commercial probiotic on the population dynamics of selected rotifers ( Brachionus angularis , Plationus patulus ) and cladocerans ( Moina cf. macrocopa and Simocephalus mixtus )

et al. 2020

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In recent decades, an increasing interest in aquaculture for producing good quality and quantity of live food has led to the use of probiotics. Rotifers and cladocerans are important as live prey for fish and invertebrate larval rearing. We carried out an experimental study to evaluate the effect of a commercial probiotic (NanoCrusta®) on improving population growth of Brachionus angularis, Plationus patulus, Moina cf. macrocopa and Simocephalus mixtus. The probiotic was tested by direct supplementation (2.1 × 10 6 , 4.2 × 10 6 , 8.5 × 10 6 and 1.7 × 10 7 cells/ml) in addition to C. vulgaris as a source of food (1 × 10 6 cells/ml). The results indicated that probiotics enhanced maximum population density (D max) and the population growth rate (r) in both zooplankton groups. At 4.2 × 10 6 cells/ml, B. angularis improved their D max (62.74%) and r (28%) compared with probiotic-free control, while P. patulus enhanced their D max and r by 51.26% and 20.83% (probiotic at 8.5 × 10 6 cells/ml). The two lowest probiotic densities resulted in an improvement in the parameters compared with the probioticfree control treatments: M. cf. macrocopa enhanced D max by 14.82% and r by 18%, and S. mixtus incremented D max 10.92% and r by 10%. The addition of probiotic bacteria also resulted in an improvement in the rotifer egg ratio (ER). K E Y W O R D S aquaculture, live food, probiotic bacteria, zooplankton | 4483 CONTRERAS-TAPIA ET Al. essential zooplankton features that meet the requirements of the early stages of development (Snell, Johnston, & Matthews, 2019; Vadstein et al., 2018). Cultured zooplankton has benefits in contrast to naturally harvested zooplankton like more stable size, shape, availability, supply, nutritional quality and digestibility (Lubzens & Zmora, 2003). Microbial community management has a positive influence on developing better zooplankton culture conditions (Tang, Turk, &

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“…Selain itu, produksi β 1,3 glukanase dari bakteri B. clausii juga memberikan potensi untuk menghidrolisis β 1-3 glukan. Produksi enzim-enzim tersebut dapat meningkatkan efisiensi penyerapan nutrisi yang sangat bermanfaat bagi rotifer (Murillo dan Villamil, 2011).…”

Section: Karakteristik Morfologi Bakteriunclassified

Bacillus sp. As a Decomposition Agent in The Maintenance of Brachionus rotundiformis Which Uses Raw Fish As a Source of Nutrition

Napitupulu¹,

Rumengan²,

Wullur³

et al. 2019

PLATAX

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The research was conducted at the Laboratory of Marine Molecular Biology and Pharmacy, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University. This research aims to isolate and analyze the morphology and molecular types of bacteria associated in rotifer’s culture media that use fisheries waste.The research was begin by culturing bacteria in rotifer maintenance media using Nutrient Broth media. After bacterial isolates were obtained, morphological characterization and DNA extraction was carried out. extraction was done using DNeasy Blood and Tissue Kit (Qiagen). After DNA was obtained, DNA was amplified through the Polymerase Reaction Chain (PCR) machine using a 16S RNA primer, followed by the separation of PCR products through electrophoresis and detection through UV Transluminator. The target PCR product was determined by comparing the 100 bp ladder DNA, with a yield of around 1400 bp, which was measured using ladder DNA available in the laboratory. The DNA that was successfully amplified was sent to be sequenced to determine the species of each microbe obtained.Based on the results of the research conducted, obtained Bacillus sp. bacteria associated with rotifer maintenance media.Keywords: Bacteria, Culture Media, DNA Extraction, PCR, Sequencing ABSTRAKPenelitian ini dilakukan di Laboratorium Biologi Molekuler dan Farmasetika Laut, Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi. Penelitian ini bertujuan untuk mengisolasi dan menganalisis morfologi dan molekuler jenis-jenis bakteri yang berasosiasi dalam media pemeliharaan rotifer yang menggunakan limbah perikanan.Penelitian dilakukan dengan cara mengkultur bakteri yang ada pada media pemeliharaan rotifer menggunakan media Nutrient Broth. Setelah isolat bakteri didapatkan, dilakukan karakterisasi morfologi dan dilakukan ekstraksi DNA. ekstraksi dilakukan menggunakan DNeasy Blood and Tissue Kit (Qiagen). Setelah DNA didapatkan, DNA diamplifikasi melalui mesin Polymerase Reaction Chain (PCR) menggunakan primer 16S RNA, diikuti dengan pemisahan produk PCR melalui electrophorisis dan deteksi melalui UV Transluminator. Produk PCR target ditentukan dengan membandingkan ladder DNA 100 bp, dengan hasil sekitar 1400 bp, yang diukur menggunakan ladder DNA yang tersedia di laboratorium. DNA yang berhasil diamplifikasi, dikirim untuk dilakukan sekuensing untuk mengetahui spesies dari setiap mikroba yang didapatkan. Berdasarkan hasil penelitian yang dilakukan, diperoleh bakteri Bacillus sp. yang berasosiasi pada media pemeliharaan rotifer. Kata Kunci: Bakteri, Media Pemeliharaan, Ekstraksi DNA, PCR, Sekuensing

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Bacillus cereus and Bacillus subtilis used as probiotics in rotifer (Brachionus plicatilis) cultures

Cited by 13 publications

References 45 publications

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis

Effect of commercial probiotic on the population dynamics of selected rotifers ( Brachionus angularis , Plationus patulus ) and cladocerans ( Moina cf. macrocopa and Simocephalus mixtus )

Bacillus sp. As a Decomposition Agent in The Maintenance of Brachionus rotundiformis Which Uses Raw Fish As a Source of Nutrition

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