2015
DOI: 10.3892/etm.2015.2333
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B7 homolog 3 aggravates brain injury in a murine model of Streptococcus pneumoniae-induced meningitis

Abstract: Abstract. Despite the application of antibiotics, Streptococcus pneumoniae (SP)-induced meningitis continues to be a life-threatening disease with a high fatality rate and an elevated risk of serious neurological sequelae, particularly in developing countries. In this study, the contribution of the co-stimulatory molecule B7 homolog 3 (B7-H3) to the pathogenesis of experimental SP-induced meningitis was investigated. Mice were challenged with the intracerebroventricular injection of serotype 3 SP with or witho… Show more

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Cited by 3 publications
(4 citation statements)
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“…Pneumococcal meningitis was induced by intracerebral ventricular injection of 15 μl sterile PBS containing 0.75×10 7 CFU/ml S . pneumoniae (SP) into the lateral ventricle as described previously [ 13 , 23 ].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Pneumococcal meningitis was induced by intracerebral ventricular injection of 15 μl sterile PBS containing 0.75×10 7 CFU/ml S . pneumoniae (SP) into the lateral ventricle as described previously [ 13 , 23 ].…”
Section: Methodsmentioning
confidence: 99%
“…pneumoniae -stimulated proinflammatory cytokine and chemokine expression in the brain via a TLR2-dependent mechanism, and this B7-H3-amplified inflammatory response further exacerbated S . pneumoniae -induced disruption of blood-brain barrier (BBB) integrity and brain damage [ 13 , 23 ], indicating that B7-H3 participates in the development of pneumococcal meningitis via augmentation of the innate immunity-associated inflammatory response.…”
Section: Introductionmentioning
confidence: 99%
“…The mice were either actively immunized and challenged with S. pneumoniae after two weeks or passively immunized and immediately challenged to establish the meningitis model. The intracerebroventricular injection method [21,22] was used, with the injection site located approximately 0.5 mm posterior to the bregma and 1.0 mm right of the midline at a vertical depth of 3.0 mm. A volume of 15 µL of the standard strain of S. pneumoniae serotype III was injected at a dose of 5 × 10 6 CFU/mouse.…”
Section: Intracerebroventricular Injection Of S Pneumoniae-induced Me...mentioning
confidence: 99%
“…The infrared detection device was utilized to capture the mice's locomotor activity, and the data analysis system was employed to collect and analyze the recorded data. The clinical disease status was examined by spontaneous motor activity [21]. The following scores were used to assess the spontaneous motor activity of mice as described previously (7): 5 points, normal motor activity and turned upright in <5 sec when put on its back; 4 points, decreased spontaneous activity, but turned upright in <5 sec; 3 points, turned upright in >5 sec; 2 points, did not turn upright; and 1 point, did not move or coma.…”
Section: Spontaneous Locomotor Activity Test In Micementioning
confidence: 99%