B16 melanoma cells increase B-1 cell survival, IL-10 production and radioresistance in vitro

Laurindo, María Fernanda Lucatelli; Thies, Felipe Garutti; Pérez, Elizabeth; Brito, Rosineide Santana de; Mariano, Mário; Popi, Ana Flávia

doi:10.1016/j.imbio.2012.07.032

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…However, since the role of B-1 in tumor growth has been poorly investigated, we cannot define whether CpG-ODN1826-induced differentiation of B-1 cells might be involved in DMPM xenograft growth inhibition. Interestingly, recent studies attributed to B-1 cells an important role in tumor progression in a melanoma model, showing that physical contact between B-1 lymphocytes and tumor cells was responsible for changes in the expression of metastasis-associated genes [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

CpG-oligodeoxynucleotides exert remarkable antitumor activity against diffuse malignant peritoneal mesothelioma orthotopic xenografts

et al. 2016

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BackgroundDiffuse malignant peritoneal mesothelioma (DMPM) is a rare and locally aggressive disease. DMPM prognosis is dismal, mainly due to the lack of effective treatment options and the development of new therapeutic strategies is urgently needed. In this context, novel immunotherapy approaches can be explored in an attempt to improve DMPM patients’ survival.MethodsWe tested the efficacy of CpG-oligodeoxynucleotides (CpG-ODN), synthetic DNA sequences recognized by Toll-like receptor 9 and able to induce innate/adaptive immune response, in two DMPM orthotopic xenografts (MesoII and STO), which properly recapitulate the dissemination pattern of the disease in the peritoneal cavity. Severe combined immunodeficiency mice carrying DMPM xenografts were treated at different stages of tumor development with i.p. delivered CpG-ODN1826 for 4 weeks. CpG-ODN1826-induced modulation in the composition of peritoneal immune infiltrate was assessed by flow cytometry.ResultsWhen administered to early-stage tumors (i.e., 4 days after i.p. DMPM cell injection in mice), the agent exhibited impressive efficacy against MesoII by completely inhibiting tumor take and ascites development (no evidence of tumor masses and ascites in 6/6 mice at necropsy), and also impaired STO tumor take and growth (4/6 tumor-free mice; i.p. tumor masses reduced by 94 % in the 2 remaining mice, P = 0.00005). Interestingly, when tested against late-stage STO tumors (i.e., 11 days after i.p. DMPM cell injection in mice), CpG-ODN1826 was still able to reduce the growth of i.p. tumor masses by 66 % (P = 0.0009). Peritoneal washings of tumor-bearing mice revealed a strong increase of macrophage infiltration together with a decrease in the presence of B-1 cells and a reduced IgM concentration after CpG-ODN1826 treatment.ConclusionsOur results indicate that locally administered CpG-ODN1826 is able to markedly affect the growth of both early- and late-stage DMPM orthotopic xenografts in the absence of severe side effects, and suggest a possible clinical role for the agent in the therapy of DMPM.

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Section: Discussionmentioning

confidence: 99%

CpG-oligodeoxynucleotides exert remarkable antitumor activity against diffuse malignant peritoneal mesothelioma orthotopic xenografts

et al. 2016

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“…Several studies investigated the origin [ 9 – 12 ], immunological properties [ 9 , 13 – 18 ] and the involvement these cells in inflammatory reactions [ 15 , 19 – 28 ]. Despite the great interest on this cell type, little is known about B-1 cells and mainly on B-1 cell derived phagocytes (B-1CDP) in models of infections by microorganisms [ 7 , 21 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

The PGE2/IL-10 Axis Determines Susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major Infection

et al. 2015

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B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. A mononuclear phagocyte derived from B-1 cells (B-1CDP) has been described. As the B-1CDP cells migrate to inflammatory/infectious sites and exhibit phagocytic capacity, the microbicidal ability of these cells was investigated using the Leishmania major infection model in vitro. The data obtained in this study demonstrate that B-1CDP cells are more susceptible to infection than peritoneal macrophages, since B-1CDP cells have a higher number of intracellular amastigotes forms and consequently release a larger number of promastigotes. Exacerbated infection by L. major required lipid bodies/PGE2 and IL-10 by B-1CDP cells. Both infection and the production of IL-10 were decreased when PGE2 production was blocked by NSAIDs. The involvement of IL-10 in this mechanism was confirmed, since B-1CDP cells from IL-10 KO mice are more competent to control L. major infection than cells from wild type mice. These findings further characterize the B-1CDP cells as an important mononuclear phagocyte that plays a previously unrecognized role in host responses to L. major infection, most likely via PGE2-driven production of IL-10.

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“…The above‐mentioned results demonstrated that CHB and primary HCC patients with HBV had significantly different peripheral neutrophil, lymphocyte, and monocyte concentrations, which have been reported to constitute the first defense barrier against cancer . As HCC patients have also been reported to possess impaired immune functions, tumor cells or their microenvironment could impair immune function . Another cohort of CHB patient data with at least 3 years of regular followup was collected to investigate the chronological sequence between HCC incidence and peripheral neutrophil, lymphocyte, and monocyte concentration alterations.…”

Section: Resultsmentioning

confidence: 99%

Low Frequency of Peripheral Lymphocyte in Chronic Hepatitis B Patients Predicts Poor Progression to Hepatocellular Carcinoma

Bao

et al. 2015

Clinical Laboratory Analysis

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B16 melanoma cells increase B-1 cell survival, IL-10 production and radioresistance in vitro

Cited by 10 publications

References 41 publications

CpG-oligodeoxynucleotides exert remarkable antitumor activity against diffuse malignant peritoneal mesothelioma orthotopic xenografts

CpG-oligodeoxynucleotides exert remarkable antitumor activity against diffuse malignant peritoneal mesothelioma orthotopic xenografts

The PGE2/IL-10 Axis Determines Susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major Infection

Low Frequency of Peripheral Lymphocyte in Chronic Hepatitis B Patients Predicts Poor Progression to Hepatocellular Carcinoma

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