THE ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) AS A SEROLOGICAL TEST FOR DETECTING ANTIBODIES AGAINSTLEPTOSPIRA INTERROGANSSEROVARHARDJOIN SHEEP

Abstract

The enzyme-liked immunosorbent assay (ELISA) was compared with the standard microscopic agglutination test (MAT) as a method for detecting antibodies against Leptospira interrogans serovar hardjo in sheep. Peak antibody levels detected by the 2 tests occurred at different times following experimental infection of sheep. In serums from flocks of sheep with naturally acquired infection there was a 95% correlation between MAT and ELISA with respect to the presence or absence of antibody to serovar hardjo, although the levels of correlation of the titres of the 2 tests was low. The 2 tests appeared to measure different antigen-antibody systems. The ELISA would be a useful test for screening large numbers of serums for antibodies to L. interrogans serovar hardjo.

Serodiagnosis of canine leptospirosis by solid-phase enzyme-linked immunosorbent assay

“…The solid-phase enzyme-linked immunosorbent assay (ELISA) has been used for the serodiagnosis of a wide range of infectious diseases in man and animals, including leptospirosis (Bruggmann et al, 1973;Voller et al, 1978;Adler et al, 1980;Terpstra et al, 1980;Adler et al, 1981). Most of the disadvantages of the MAT do not apply to the ELISA.…”

Leptospirosis

“…In contrast to the applications of ELISA for diagnosis of human infection, in which broadly reactive assays are generally desirable and few serovar-specific assays have been developed (395), veterinary applications have been directed towards detection of serovar-specific antibodies, particularly for detection of infection in food animals. ELISA methods have described for detection of serovar pomona (134,573) and hardjo (5,58,573,653) infection in cattle and hardjo in sheep (9). Several assays are available commercially for serodiagnosis of bovine hardjo infection and have been evaluated (642).…”

Detection of leptospiral antibodies by microscopic agglutination test in north–east of Iran

“…The enzyme-linked immunosorbent assay (ELISA) and the MAT is the laboratory methods generally used. The ELISA technique has been compared with the MAT for the diagnosis of leptospirosis in human, cattle and cousins which indicated that there is high correlation between the ELISA and MAT results [4][5][6] .…”