Serodiagnosis of canine leptospirosis by solid-phase enzyme-linked immunosorbent assay

Hartman, E.G.; Houten, M. van; Donk, J.A. van der; Frik, J. F.

doi:10.1016/0165-2427(84)90025-4

Cited by 23 publications

(6 citation statements)

References 13 publications

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“…Furthermore, it is thought that ELISA detects both agglutinating and non-agglutinating antibodies, whereas only the former are detected by MAT. Indeed, the detection of nonagglutinating Leptospira antibodies in the sera of dogs in the early stage of infection by an indirect ELISA has been reported [9]. It is therefore possible in the present study that ELISA detected non-agglutinating antibodies.…”

Section: Discussionsupporting

confidence: 46%

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Iwamoto

Wada

Fujisaki

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Leptospirosis is an infectious disease caused by Leptospira interrogans sensu lato and is common in both humans and animals. In the present study, serum samples were collected from 801 dogs across all 47 prefectures in Japan, and evaluated with a microscopic agglutination test (MAT), using 5 major L. interrogans serovars (Icterohaemorrhagiae, Canicola, Autumnalis, Hebdomadis, and Australis) as antigens, and an enzyme-linked immunosorbent assay (ELISA) using recombinant OmpL1 protein as the antigen. Across all dogs tested, 217 (27.0%) and 29 (3.6%) were MAT-and ELISA-positive, respectively. However, evidence strongly suggests that MAT also detected antibodies produced by vaccination. Of 243 dogs never inoculated with any canine vaccine, 41 (16.9%) from 23 prefectures were MAT and/or ELISA positive. The most commonly detected serovar was Icterohaemorrhagiae (22 dogs, 19 prefectures). Our results suggest that there are dogs with subclinical Leptospira infection throughout Japan. To the best of our knowledge, the present study is the first nationwide survey of Leptospira infection in dogs, and the findings are relevant not only for clinical veterinary medicine but also for public health.

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Section: Discussionsupporting

confidence: 46%

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Iwamoto

Wada

Fujisaki

et al. 2009

The Journal of Veterinary Medical Science

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“…Several assays are available commercially for serodiagnosis of bovine hardjo infection and have been evaluated (642). IgM detection by ELISA has also been applied to canine diagnosis (264,265,623).…”

Section: Downloaded Frommentioning

confidence: 99%

Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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“…At this time, the only licensed canine vaccines in New Zealand are monovalent and contain serovar Icterohaemorrhagiae, which cross-reacts immunologically with serovar Copenhageni (Hartman et al 1984;Schoone et al 1989). Thus a vaccine that provides protection against serovars Pomona and Hardjo is sorely needed.…”

Section: Introductionmentioning

confidence: 95%

The serological response of working farm dogs to a vaccine containingLeptospira interrogansserovars Copenhageni and Pomona, andL. borgpeterseniiserovar Hardjo

Cave

Harland

Allott³

2013

New Zealand Veterinary Journal

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There has been a recent increase in cases of leptospirosis attributed to the serovar Pomona in dogs in New Zealand, but the vaccines licensed for use in dogs in New Zealand for the prevention of leptospirosis only protect against Copenhageni/Icterohaemorrhagiae. The vaccine tested in this study produced titres to Pomona and Hardjo that suggest the vaccine could be useful for reducing disease caused by these serovars in working dogs in New Zealand.

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Serodiagnosis of canine leptospirosis by solid-phase enzyme-linked immunosorbent assay

Cited by 23 publications

References 13 publications

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Leptospirosis

The serological response of working farm dogs to a vaccine containingLeptospira interrogansserovars Copenhageni and Pomona, andL. borgpeterseniiserovar Hardjo

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