The cytotoxic T cells may contribute to thein situimmune response in Jorge Lobo's Disease human lesions

Abstract

Jorge Lobo's Disease (JLD) is a cutaneous chronic granulomatous disease caused by the pathogenic fungus Lacazia loboi. It is characterized by a granulomatous reaction with multinucleated giant cells and high number of fungal cells. In order to contribute to the comprehension of immune mechanisms in JLD human lesions, we studied the cytotoxic immune response, focusing on TCD8+ and NK cells, and granzyme B. Forty skin biopsies of lower limbs were selected and an immunohistochemistry protocol was developed to detect CD8+ T cells, NK cells and Granzyme B. In order to compare the cellular populations, we also performed a protocol to visualize TCD4+ cells. Immunolabeled cells were quantified in nine randomized fields in the dermis. Lesions were characterized by inflammatory infiltrate of macrophages, lymphocytes, epithelioid and multinucleated giant cells with intense number of fungal forms. There was a prevalence of CD8 over CD4 cells, followed by NK cells. Our results suggest that in JLD the cytotoxic immune response could represent another important mechanism to control Lacazia loboi infection. We may suggest that, although CD4+ T cells are essential for host defense in JLD, CD8+ T cells could play a role in the elimination of the fungus.

TLR3 Is a Negative Regulator of Immune Responses Against Paracoccidioides brasiliensis

“…In accordance, Alexandre and coworks showed that the cytotoxic immune response was a mechanism to control Lacazia loboi infection. They suggested that CD8 + T cells could play a role in the elimination of the fungus (Alexandre et al, 2017).…”

Lacaziosis: immunohistochemical evaluation of elements of the humoral response in cutaneous lesions

“…The cytotoxic function of CD8+ T cells was evidenced based on the detection of perforin and granzyme B and a role in the in situ immune response to eliminate the fungus was suggested. Similarly, by studying the cytotoxic cellular response in such lesions, we highlight and suggest an important role of these cytotoxic elements 7 .…”

Análise comparativa do perfil citocínico Th22 na imunopatogenia da doença de Jorge Lobo e cromomicose

“…Concluindo-se que, embora as células T CD4+ sejam essenciais para a defesa do hospedeiro na DJL, as células T CD8+ poderiam desempenhar um papel na eliminação do fungo (Alexandre et al, 2017). No que se refere à CBM, as condições imunológicas do hospedeiro, a virulência, viabilidade e quantidade de parasita são fatores determinantes do curso da infecção.…”