<b>Neutrophil Migration During Liver Cirrhosis In Rabbits</b>

Penteado, F. C.; Ferreira, H. H. A.; Calafatti, S. C.; Bernascconi, G.; Piovesana, H.; Pedrazzoli, José

doi:10.1046/j.1440-1681.2002.03646.x

Cited by 4 publications

(8 citation statements)

References 30 publications

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“…A source of reactive oxygen in the liver can be infiltrating neutrophils, where these cells are systematically activated and accumulate in the liver before excessive liver injury. 35,36 In the present study the acid did not alter the myeloperoxidase activity of the liver by either subcutaneous or gavage administration, and this result suggests that the acid has no marked effect on neutrophil recruitment by the liver. Oxidative stress is presumed to play an important role in hepatic fibrogenesis and several report of liver pathology show a significant alteration of cellular structure.…”

Section: Resultssupporting

confidence: 49%

Influence of indole acetic acid on antioxidant levels and enzyme activities of glucose metabolism in rat liver

Oliveira

Pugine

Ferreira

et al. 2005

Cell Biochemistry & Function

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Indole acetic acid (IAA) is an auxin and can be synthesized in animals. This compound is metabolized in vitro by peroxidase, producing reactive oxygen species. The toxic effect of indole acetic acid in leukocytes is associated with peroxidase activities and these processes have been implicated in activation of glucose and glutamine metabolism. However, studies in vitro have shown that IAA, in absence of peroxidase, is an antioxidant almost as high in potency as those of other indolic compounds. The purpose of this study was to investigate the possible involvement of a toxic effect of indole acetic acid in the liver, as evidenced by oxidative stress and enzyme activities of the glucose pathway. The animals received IAA by subcutaneous or gavage administration in a phosphate buffered saline (the control group received only the phosphate buffered saline). The other groups received IAA at concentrations of 1 mg, 18 mg and 40 mg per kg of body mass per day. Treatments with 18 mg and 40 mg IAA decreased the activity of catalase by both subcutaneous (30% and 26%) or gavage administration (19% and 28%), respectively. A similar effect was observed on the activity of glutathione peroxidase of animals exposed to 18 mg and 40 mg IAA: A decrease of 34% and 29%, respectively, for subcutaneous administration and a decrease of 29% and 25%, respectively, for gavage administration. However, in neither source of administration did the acid alter superoxide dismutase, glutathione reductase and myeloperoxidase activities. Another alteration was observed in respect of reduced glutathione content in this organ. The lipid peroxidation level showed a significant decrease with subcutaneous (30%, 29% and 24%) and gavage administration (25%, 26% and 24%) using 1 mg, 18 mg and 40 mg of IAA, respectively compared with the control. The reduced glutathione content and catalase activity in the plasma were not altered by either of the two methods of administration. In addition to these findings, after subcutaneous or gavage administration of IAA, the activities of hepatic enzymes of glucose metabolism were not affected (glucokinase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and citrate synthase). Evidence is presented herein that IAA did not have a pro-oxidant effect in the liver as deduced from a reduction of catalase and glutathione peroxidase activities, a decrease of lipid peroxidation content and no alteration of the pool of reduced glutathione. The effects of IAA were independent of the way of administration.

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Section: Resultssupporting

confidence: 49%

Influence of indole acetic acid on antioxidant levels and enzyme activities of glucose metabolism in rat liver

Oliveira

Pugine

Ferreira

et al. 2005

Cell Biochemistry & Function

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“…In our rabbit model after 19 weeks of CCl 4 treatment the histological picture and clinical aspects are consistent with experimental cirrhosis and the findings of other authors. 24,25 The present results show that experimental hepatic damage induced by prolonged treatment with CCl 4 presents an anatomopathological picture of postnecrotic cirrhosis according to aetiopathogenic classification of the disease. 2,3 Parenchymal normal architecture was largely changed, with destruction of sublobular or -sometimes -lobular areas.…”

Section: Discussionsupporting

confidence: 52%

“…In the original description of the model, Penteado et al 25 determined the CCl 4 dose on the basis of transaminase levels. However, AST and ALT levels do not always parallel each other (Table 2).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the spontaneous reversibility of carbon tetrachloride-induced liver cirrhosis in rabbits

et al. 2012

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There is a general consensus that liver fibrosis in humans is potentially reversible, while scepticism prevails on the concept that cirrhosis can be truly reversed. The availability of suitable experimental models is fundamental for disease research. The experimental murine model of liver cirrhosis induced by carbon tetrachloride (CCl(4)) reproduces both the histological picture of the postnecrotic cirrhosis and its biochemical and clinical parameters. Normal hepatic structure is modified by formation of regeneration nodules. Fibrosis represents a morphological element of disease and an effect of hepatocyte necrosis. However, the relevance for research of this well-established model of liver cirrhosis is hampered by some spontaneous cirrhosis regression reported in mice and rats. It has been reported that CCl(4) also induces experimental liver cirrhosis in rabbits, but it is not known whether the process is reversible in this species. The aim of our study was to investigate this question. Male New Zealand White rabbits were treated intragastrically with CCl(4) or the vehicle only for 19 weeks and groups were sacrificed three and five months after treatment interruption. Cirrhotic and control livers were processed for routine light microscopy and for morphometric study of fibrosis by semiquantitative evaluation. The degree of fibrosis was based on the Knodell's scoring system.

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“…1 Photomicrograph of a 5 lm liver section stained with H&E, representative of (a) control group, b proanthocyanidin group, c, d gibberellic acid group, e indoleacetic acid group, f gibberellic acid and proanthocyanidin group and g indoleacetic acid and proanthocyanidin group (Scale bar represents 400 lm). BN binucleatd hepatocytes, BS bile sinusoids, CBV congested blood vessel, CV central vein, DBS dilatation in bile sinusoids, DHS disturbed hepatic strands, EN normal endothelium, GH giant hepatic cells, HC hepatocytes, HS hepatic strands, K karyoryxis, KC Kupffer cells, LI lymphocytic infiltration, PKN pyknotic nuclei, V vacuolation b Cytotechnology (2014) 66:585-596 589 reports of liver pathology show a significant alteration of cellular structure (Penteado et al 2002;Sarandol et al 2005). The administration of proanthocyanidin (PAC) caused significant histopathological improvement in hepatic and testicular tissues.…”

Section: Discussionmentioning

confidence: 99%

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

2013

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The aim of the present study was to investigate the protective role of grape seed extract (containing proanthocyandin) against the adverse effects of plant growth regulators (GA 3 (gibberellic acid) and IAA (indoleacetic acid)). The present data showed that the administration of either GA 3 and IAA caused undesirable changes in both hepatic and testicular structure. This was evidenced by a disturbed hepatic strands, pyknotic nuclei, central vein with collapsed endothelium, dilatation in bile sinusoids, congested blood vessel, binucleatd hepatocytes, lymphocytic infiltration, vacuolation, giant hepatic cells, increased Kupffer cells and karyoryxis. Additionally, it was shown that degenerative changes in the testis, spermatogenic arrest, moderate tubular necrosis, Leydig cell degeneration and reduction in the number and size of the seminiferous tubules with some spermatogonia detached from the basement membrane. Concerning flow cytometric study of the liver a significant decrease in G0/1 % and a significant increase in S phase %, G2/ M %, P 53 % and apoptosis % (sub G1) were detected. However, in testis the data recorded a significant decrease in the percentage of mature sperm (percentage of haploid cells) and a significant increase in the percentage of spermatide, diploid cells, P 53 and of apoptotic cells. On the other hand, a distinct recovery of the mentioned hepatic and testicular histopathological and cytogenetic disorders was observed when proanthocyanidin was supplemented to rats administered either of the plant growth hormones (GA 3 and IAA).

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Neutrophil Migration During Liver Cirrhosis In Rabbits

Cited by 4 publications

References 30 publications

Influence of indole acetic acid on antioxidant levels and enzyme activities of glucose metabolism in rat liver

Influence of indole acetic acid on antioxidant levels and enzyme activities of glucose metabolism in rat liver

Evaluation of the spontaneous reversibility of carbon tetrachloride-induced liver cirrhosis in rabbits

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

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