Map positions ofSFRgenes in relation to other freezing‐related genes ofArabidopsis thaliana

Thorlby, Glenn; Veale, Emma L.; Butcher, Kevin; Warren, Gareth J.

doi:10.1046/j.1365-313x.1999.00395.x

Cited by 24 publications

(16 citation statements)

References 66 publications

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“…Factors controlling either the protection of cell structures or the recovery from damage might determine the difference between the two genotypes, Ler and Cvi. In particular, some of the QTL that we have identified for viability after CDT colocated with a cluster of genes related to stress tolerance on the top of chromosome 1 (Taji et al, 1999;Thorlby et al, 1999). Further fine mapping of these loci, combined with the analysis of other related traits, will allow the identification of the corresponding genes involved in the storability genetic variation.…”

Section: Discussionmentioning

confidence: 89%

Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to Seed Storability of Arabidopsis

et al. 2000

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(K.T., S.P.C.G.)Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance and consequently in seed storability. In the present work we analyzed the seed-soluble OS (sucrose, raffinose, and stachyose) content of several Arabidopsis accessions and thus identified the genotype Cape Verde Islands having a very low RSO content. By performing quantitative trait loci (QTL) mapping in a recombinant inbred line population, we found one major QTL responsible for the practically monogenic segregation of seed stachyose content. This locus also affected the content of the two other OSs, sucrose, and raffinose. Two candidate genes encoding respectively for galactinol synthase and raffinose synthase were located within the genomic region around this major QTL. In addition, three smaller-effect QTL were identified, each one specifically affecting the content of an individual OS. Seed storability was analyzed in the same recombinant inbred line population by measuring viability (germination) under two different seed aging assays: after natural aging during 4 years of dry storage at room temperature and after artificial aging induced by a controlled deterioration test. Thus, four QTL responsible for the variation of this trait were mapped. Comparison of the QTL genetic positions showed that the genomic region containing the major OS locus did not significantly affect the seed storability. We concluded that in the studied material neither RSOs nor sucrose content had a specific effect on seed storability.In many plants species, including Arabidopsis (Ooms et al., 1993), seed maturation is accompanied by the accumulation of soluble oligosaccharides (OSs) (Horbowicz and Obendorf, 1994). These OSs, mainly Suc and raffinose series oligosaccharides (RSOs) are found in cotyledons, seed coats, and hypocotyls (Obendorf, 1997 and references therein). RSOs are derivatives of Suc to which Gal units are added to the Glc moiety of Suc through ␣-(1,6) bonds. Raffinose contains one Gal unit, whereas stachyose has two such units. RSOs appear during the later stages of seed development and they disappear upon germination (Obendorf, 1997). In Arabidopsis, seeds of the accession Landsberg erecta (Ler) accumulate raffinose and especially stachyose at the later stages of seed development, whereas the Suc content remains constant (Ooms et al., 1993). These three OSs together represent approximately 2% of the dry weight of mature seeds.Studies in several species such as soybean (Glycine max), maize (Zea mays), and brassica (Brassica campestris [rapa]) have suggested that OSs might be involved in the protection of seeds against damage during seed dehydration and aging, and therefore in seed survival and storability (for review, see Obendorf, 1997;Sinniah et al., 1998). OSs have been speculated to be involved in the protection of membranes, proteins, and nucleic acids against damage that occurs during and upon the withdrawal of water in the drying seeds (Ho...

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Section: Discussionmentioning

confidence: 89%

Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to Seed Storability of Arabidopsis

et al. 2000

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“…Several other stress-related genes were mapped in this region, e.g. genes related to freezing tolerance, possibly also related to oxidative stress (Thorlby et al, 1999), and genes involved in drought stress, one of them having homology to a glutathione S-transferase, an enzyme involved in reactive oxygen scavenging (Taji et al, 1999). However, germination on H 2 O 2 could not confirm the relationship between ROS and germination.…”

Section: Ros As a Common Factormentioning

confidence: 99%

Analysis of Natural Allelic Variation of Arabidopsis Seed Germination and Seed Longevity Traits between the Accessions Landsberg erecta and Shakdara, Using a New Recombinant Inbred Line Population

et al. 2004

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Quantitative trait loci (QTL) mapping was used to identify loci controlling various aspects of seed longevity during storage and germination. Similar locations for QTLs controlling different traits might be an indication for a common genetic control of such traits. For this analysis we used a new recombinant inbred line population derived from a cross between the accessions Landsberg erecta (Ler) and Shakdara (Sha). A set of 114 F9 recombinant inbred lines was genotyped with 65 polymerase chain reaction-based markers and the phenotypic marker erecta. The traits analyzed were dormancy, speed of germination, seed sugar content, seed germination after a controlled deterioration test, hydrogen peroxide (H 2 O 2 ) treatment, and on abscisic acid. Furthermore, the effects of heat stress, salt (NaCl) stress, osmotic (mannitol) stress, and natural aging were analyzed. For all traits one or more QTLs were identified, with some QTLs for different traits colocating. The relevance of colocation for mechanisms underlying the various traits is discussed.

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“…The sfr2-1 mutation was previously mapped to the region between markers GAPC and nga126 on chromosome III (Thorlby et al, 1999). From the mapping cross of sfr2-1/sfr2-1 Columbia (Col) 3 SFR2 þ /SFR2 þ Landsberg erecta, we screened 690 F2 zygotes for crossovers in this region.…”

Section: Classical Genetic Mapping Of the Sfr2 Genementioning

confidence: 99%

“…DNA samples were isolated from F2 plants (Thorlby et al, 1999) and analyzed for cleaved amplified polymorphic sequence marker genotypes (Konieczny and Ausubel, 1993) and simple sequence length polymorphism marker genotypes (Bell and Ecker, 1994) as described. One restriction fragment length polymorphism marker, mi403, was also used.…”

Section: Generation and Use Of Physical Markers In Mappingmentioning

confidence: 99%

The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a β-Glucosidase

2004

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The sensitive to freezing2-1 (sfr2-1) mutation causes freezing sensitivity in Arabidopsis thaliana. By mapping, transgenic complementation, and sequencing, sfr2-1 was revealed to be a mutation in gene At3g06510. A new knockout allele was obtained, and its identical freezing-sensitive phenotype confirmed that the SFR2 gene product is essential for freezing tolerance. Transcription of SFR2 was observed to be constitutive rather than stress inducible and was distributed throughout most aerial tissues. SFR2 encodes a protein homologous to family 1 glycosyl hydrolases (b-glycosidases), but the predicted AtSFR2 protein is divergent from all other family 1 b-glycosidases of Arabidopsis, showing closer homology to the sequences of several b-glycosidases from thermophilic archea and bacteria. After purification from a heterologous expression system, AtSFR2 displayed a specific hydrolytic activity against b-D-glucosides.

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Map positions ofSFRgenes in relation to other freezing‐related genes ofArabidopsis thaliana

Cited by 24 publications

References 66 publications

Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to Seed Storability of Arabidopsis

Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to Seed Storability of Arabidopsis

Analysis of Natural Allelic Variation of Arabidopsis Seed Germination and Seed Longevity Traits between the Accessions Landsberg erecta and Shakdara, Using a New Recombinant Inbred Line Population

The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a β-Glucosidase

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