In vivo proteome dynamics during early bovine myogenesis

Abstract

Myogenesis is a complex process of which the underlying mechanisms are conserved between species, including birds and mammals. Despite a good understanding of the stages of myogenesis, many of the mechanisms involved in the regulation of proliferation of the successive myoblast generations, the cellular transitions cell proliferation/alignment of myoblasts/fusion of myoblasts into myotubes/differentiation of myofibres and the control of total myofibre number still remain unknown. An in vivo proteomic analysis of the semitendinosus muscle from Charolais foetuses, at three specific stages of myogenesis (60, 110 and 180 days postconception), was conducted using 2-DE and MS. Expression profiles of more than 170 proteins were revealed and analysed using two way hierarchical clustering and statistical analysis. Our studies identify, for the first time, distinct proteins of varied biological functions and protein clusters with myogenic processes, such as the control of cell cycle activity and apoptosis, the establishment of cellular metabolism and muscle contractile properties and muscle cell reorganisation. These results are of fundamental interest to the field of myogenesis in general, and more specifically to the control of muscle development in meat producing animals.

Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle

“…Thus, changes in proportions of Alpha and Beta enolase in bovine muscle are in total agreement with the finding of Fougerousse et al (2001) on human muscle. Muscle-specific Enolase (Beta enolase) is detected very early in bovine myogenesis, at 60 dpc (Chaze et al, 2008), the relative abundance of Beta enolase from 180 to 260 dpc increased significantly, while during the same time relative abundance of Alpha enolase decreases slightly.…”

“…Completed with earlier work (Chaze et al, 2008), this larger study could be of particular interests for the study of human muscular diseases and to gain knowledge of early human myogenesis.…”

“…This will allow us to have a complete description of the modifications of protein profiles during the differentiation phase and to reveal some proteins involved in the control of differentiation. This will complete an earlier study concerning the early stages of bovine myogenesis from 60 to 180 dpc, corresponding to proliferation stages (Chaze et al, 2008).…”

Proteomic profiling of bovine M. longissimus lumborum from Crossbred Aberdeen Angus and Belgian Blue sired steers varying in genetic merit for carcass weight1

“…Bovine skeletal muscle is a heterogeneous tissue comprised of several fi ber types infl uenced by the genotype and breed of the animal (Bouley et al, 2005;Chaze et al, 2008). Additionally, its properties evolve during postnatal life and can be modifi ed by environmental conditions (Therkildsen, 2005;Shibata et al, 2009).…”

Differential proteome and transcriptome analysis of porcine skeletal muscle during development

“…The both proteins were highly expressed in 65 dpc LM, which might help in the role of maintaining proper alignment of cellular structures during physiological functioning of muscle. We also demonstrated the high-level expression of gelsolin, cofilin-1 and FSCN1 in 65 dpc, which regulated actin dynamics and were targets of many signaling pathways affecting the actin cytoskeleton [50][51][52]. Another protein, sarcosin, also called kelch-related protein 1, is a cardiac and skeletal muscle kelch repeat protein hypothesized to promote the assembly of myofibrils and associated with cytoskeletal elements [53].…”