Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle

Chaze, Thibault; Meunier, Bruno; Chambon, Christophe; Jurie, Catherine; Picard, Brigitte

doi:10.1017/s1751731109004315

Cited by 22 publications

(33 citation statements)

References 34 publications

(48 reference statements)

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“…Charolais foetuses were generated by artificial insemination of Charolais cows with the semens of two Charolais bulls as reported earlier (Chaze et al, 2009). They were collected from cows slaughtered at the Experimental INRA slaughterhouse at pregnancy days 60 (n 5 4), 110 (n 5 4), 180 (n 5 4), 210 (n 5 4) and 260 (n 5 4).…”

Section: Experimental Designsmentioning

confidence: 99%

Expression of DNAJA1 in bovine muscles according to developmental age and management factors

Cassar-Malek

Guillemin

Hocquette

et al. 2011

Animal

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We have recently shown that the expression of the DNAJA1 gene encoding a heat shock protein (Hsp40) is a negative marker of meat tenderness in Charolais bulls. To acquire knowledge on the regulation of DNAJA1 expression, we analysed the abundance of DNAJA1 transcripts and protein during development and according to management factors (e.g. feeding treatments, growth path and stress status) in different bovine muscles during postnatal life. We report here a developmental expression profile for DNAJA1 with decreased levels of transcript and protein during the progression of myogenesis. During postnatal life, we found the highest expression of DNAJA1 in the most oxidative muscles. No effect was detected for dietary treatment (pasture v. maize-based diet), growth path (compensatory growth after a restriction period) or pre-slaughter stress status. Therefore, the genetic background and muscle type could be considered as the main factors regarding the level of DNAJA1. Integration of the knowledge gained from this study should help to predict muscle metabolic properties and the ability of the live animals to give high sensory quality meat.Keywords: Hsp40, myogenesis, nutrition, growth path, stress ImplicationsBeef is characterized by a high and uncontrolled variability of its sensory attributes, especially tenderness, which is one reason for consumer dissatisfaction. There is still no simple, reliable and reproducible technique to predict quality, which is a limitation to the delivery of a consistent quality meat. Thus, identification of markers of quality attributes is of major socio-economic importance. Recently, we reported that expression of DNAJA1 was a negative tenderness marker. This study examines DNAJA1 expression in bovine muscles having different metabolic specificities during development and according to management factors. It should help to increase knowledge of DNAJA1 expression, and to screen for live animals with favourable muscle properties and potentially high-quality meat.

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Section: Experimental Designsmentioning

confidence: 99%

Expression of DNAJA1 in bovine muscles according to developmental age and management factors

Cassar-Malek

Guillemin

Hocquette

et al. 2011

Animal

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“…1). The distribution of protein spots was broadly similar to patterns found in skeletal muscle from other vertebrates [38,39]. Using image software, protein spots were quantified on every gel at every stage.…”

Section: 2mentioning

confidence: 69%

Differential proteome and transcriptome analysis of porcine skeletal muscle during development

Qian

Feng

et al. 2012

Journal of Proteomics

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“…These results are consistent with those of similar bovine studies (Savill & Fadok ; Chaze et al . , ).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide proteomics analysis on longissimus muscles in Qinchuan beef cattle

Chen

Liang

et al. 2016

Animal Genetics

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To gain further insight into the molecular mechanism of bovine muscle development, we combined mass spectrometry characterization of proteins with Illumina deep sequencing of RNAs obtained from bovine longissimus muscle (LD) at prenatal and postnatal stages. For the proteomic study, each group of LD proteins was extracted and labeled using isobaric tags for relative and absolute quantitation (iTRAQ) method. Among the 1321 proteins identified from six samples, 390 proteins were differentially expressed in embryos at day 135 post-fertilization (Emb135d) vs. 30-month-old adult cattle (Emb135d vs. 30M) samples. Gene Ontology, Cluster of Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes analyses were further conducted to better understand the different functions. Furthermore, we analyzed the relationship between transcript and protein regulation between samples by direct comparison of expression levels from transcriptomic and iTRAQ-based proteomics. Association results indicated that 1295 of 1321 proteins could be mapped to transcriptome sequencing data. This study provides the most comprehensive, targeted survey of bovine LD proteins to date and has shown the power of combining transcriptomic and proteomic approaches to provide molecular insights for understanding the developmental characteristics in bovine muscle, and even in other mammals.

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Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle

Cited by 22 publications

References 34 publications

Expression of DNAJA1 in bovine muscles according to developmental age and management factors

Expression of DNAJA1 in bovine muscles according to developmental age and management factors

Differential proteome and transcriptome analysis of porcine skeletal muscle during development

Genome‐wide proteomics analysis on longissimus muscles in Qinchuan beef cattle

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