Escherichia coli RNase HI inhibits murine leukaemia virus reverse transcription in vitro and yeast retrotransposon Ty1 transposition in vivo

Ma, Wu‐Po; Crouch, Robert J.

doi:10.1046/j.1365-2443.1996.d01-265.x

Cited by 11 publications

(9 citation statements)

References 32 publications

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“…A hyperactive RNH might result in the premature or incorrect processing of the PPT, which would be lethal to the element. Consistent with this, the introduction of the E. coli RNH domain is inhibitory to the retrotransposition of LTR retrotransposons including vertebrate retroviruses (Ma and Crouch, 1996). On the other hand, there appears to be only a minimal cost associated with a drop in RNH activity since it does not appear to be rate-limiting (Sevilya et al, 2003).…”

Section: Rnh Evolution In Retroelementsmentioning

confidence: 73%

Ribonuclease H evolution in retrotransposable elements

Malik

2005

Cytogenet Genome Res

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Eukaryotic and prokaryotic genomes encode either Type I or Type II Ribonuclease H (RNH) which is important for processing RNA primers that prime DNA replication in almost all organisms. This review highlights the important role that Type I RNH plays in the life cycle of many retroelements, and its utility in tracing early events in retroelement evolution. Many retroelements utilize host genome-encoded RNH, but several lineages of retroelements, including some non-LTR retroposons and all LTR retrotransposons, encode their own RNH domains. Examination of these RNH domains suggests that all LTR retrotransposons acquired an enzymatically weak RNH domain that is missing an important catalytic residue found in all other RNH enzymes. We propose that this reduced activity is essential to ensure correct processing of the polypurine tract (PPT), which is an important step in the life cycle of these retrotransposons. Vertebrate retroviruses appear to have reacquired their RNH domains, which are catalytically more active, but their ancestral RNH domains (found in other LTR retrotransposons) have degenerated to give rise to the tether domains unique to vertebrate retroviruses. The tether domain may serve to control the more active RNH domain of vertebrate retroviruses. Phylogenetic analysis of the RNH domains is also useful to “date” the relative ages of LTR and non-LTR retroelements. It appears that all LTR retrotransposons are as old as, or younger than, the “youngest” lineages of non-LTR retroelements, suggesting that LTR retrotransposons arose late in eukaryotes.

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Section: Rnh Evolution In Retroelementsmentioning

confidence: 73%

Ribonuclease H evolution in retrotransposable elements

Malik

2005

Cytogenet Genome Res

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“…QTL mapping resolution depends on marker density and the size of the confidence interval of QTLs (Visscher et al, 1996;Da et al, 2000). Generally, a gain in information as a result of increased marker density results in smaller intervals; therefore, the application of additional markers is an effective way to increase QTL mapping resolution (Liu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Trait Locus Analysis of Seed Germination and Early Seedling Growth in Rice

Yang

et al. 2019

Front. Plant Sci.

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Seed germination and early seedling growth are important agricultural traits for developing populations of both irrigated and directly seeded rice (DSR). To investigate the genetic mechanisms underlying seed germination and early seedling growth in rice, 275 recombinant inbred lines (RILs) were genotyped in this study via the genotyping-bysequencing (GBS) approach to construct a high-density linkage bin map based on the parent-independent genotyping method. Quantitative trait loci (QTLs) for 12 traits related to seed germination and early seedling growth were analyzed. Totally, 22 additive loci were detected, after analysis of the interaction between additive QTLs and environments, five stable additive loci were obtained. Among them, loci 4, 5, 12 and 14 exhibited clear pleiotropic effects that were associated with multiple traits. Analysis of the effects of the five additive stable loci showed that a single locus increased the corresponding phenotypic value. Ten of the 275 RILs pyramided the excellent alleles of the five stable genetic loci. Most phenotypic values of the ten RILs were greater than the average values. Four RILs (G260, G342, G371, and G401) with more excellent phenotypic values were subsequently selected; these RILs could serve as donor parents of favorable alleles in the breeding process. Due to the existence of pleiotropy, the use of these genetic loci for pyramid breeding can further increase the efficiency to reach breeding goals. In addition, these five stable loci have an average physical interval of only 170 kb, we also further identified five promising candidate genes by qRT-PCR, which provides us with a basis for future cloning of these genes. Overall, this work will help broaden our understanding of the genetic control of seed germination and early seedling growth, and this study provides both a good theoretical basis and a new genetic resource for the breeding of direct-seeded rice.

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“…This is consistent with the difficulty in purifying VLPs composed of C‐terminal His‐tagged Tya proteins on a nickel column in non‐denaturing conditions (Roth, unpublished data). Incorporation of nucleases into VLPs via C‐terminal Gag or Gag/Pol fusion interferes with retrotransposition but not VLP assembly, providing further evidence that the C‐terminus is indeed inside the particle 47, 59…”

Section: Function Of Tya During Assemblymentioning

confidence: 98%

The yeast Ty virus-like particles

Roth

2000

Yeast

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***Escherichia coli* RNase HI inhibits murine leukaemia virus reverse transcription in vitro and yeast retrotransposon Ty1 transposition *in vivo***

Cited by 11 publications

References 32 publications

Ribonuclease H evolution in retrotransposable elements

Ribonuclease H evolution in retrotransposable elements

Quantitative Trait Locus Analysis of Seed Germination and Early Seedling Growth in Rice

The yeast Ty virus-like particles

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