The yeast Ty virus-like particles

Roth, J

doi:10.1002/1097-0061(20000630)16:9<785::aid-yea550>3.0.co;2-l

Cited by 55 publications

(29 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This treatment has been shown to destabilize Ty1 VLPs (Roth 2000), as monitored by the amount of capsid protein in the VLP pellet vs. the supernatant. However, the sedimentation profile of VLPs from the wild type or the bud22D mutant remained unchanged after treatment with Tris-HCl, suggesting that VLPs from the bud22D mutant are stable.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

BUD22 Affects Ty1 Retrotransposition and Ribosome Biogenesis in Saccharomyces cerevisiae

et al. 2010

View full text Add to dashboard Cite

A variety of cellular factors affect the movement of the retrovirus-like transposon Ty1. To identify genes involved in Ty1 virus-like particle (VLP) function, the level of the major capsid protein (Gag-p45) and its proteolytic precursor (Gag-p49p) was monitored in a subset of Ty1 cofactor mutants. Twenty-nine of 87 mutants contained alterations in the level of Gag; however, only bud22D showed a striking defect in Gag processing. BUD22 affected the 11 translational frameshifting event required to express the Pol proteins protease, integrase, and reverse transcriptase. Therefore, it is possible that the bud22D mutant may not produce enough functional Ty1 protease to completely process Gag-p49 to p45. Furthermore, BUD22 is required for 18S rRNA processing and 40S subunit biogenesis and influences polysome density. Together our results suggest that BUD22 is involved in a step in ribosome biogenesis that not only affects general translation, but also may alter the frameshifting efficiency of ribosomes, an event central to Ty1 retrotransposition.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Ty1-VLP integrity: This qualitative assay was modified from one previously described (McCarthy et al 1998;Roth 2000). Briefly, an aliquot of a VLP preparation (60 mg) was mixed with VLP disruption buffer (250 mm Tris-HCl, pH 7.4, 15 mm KCl, 10 mm HEPES-KOH, pH 7.4, and 5 mm EDTA) in a total volume of 100 ml.…”

Section: Methodsmentioning

confidence: 99%

BUD22 Affects Ty1 Retrotransposition and Ribosome Biogenesis in Saccharomyces cerevisiae

et al. 2010

View full text Add to dashboard Cite

show abstract

“…1,2 Ty1 retrotransposons contain two partially overlapping ORFs, TYA1 and TYB1, that encode for TyA1/Gag and TyB1/Pol proteins (hereafter denoted Gag and Pol), but lack a functional homolog of the Env protein, essential for infectivity. 3,4 While Gag can be readily translated as an immature Gag-p49 form, Pol synthesis requires a ribosomal frameshift, creating a template for the production of a Gag-Pol fusion protein that is further processed by proteolysis. 5,6 Similar to retroviruses, Ty1 genomic RNA, Gag and unprocessed Gag-Pol assemble to form immature VLPs.…”

Section: Introductionmentioning

confidence: 99%

T-body formation precedes virus-like particle maturation inS. cerevisiae

Malagón

Jensen

2011

RNA Biology

View full text Add to dashboard Cite

“…Production of HBsAgVLPs has been performed using P. pastoris [42,43]. Apart from virus VLPs, theTy1 and Ty3 particles were observed intracellularly in yeast and these particles have a diameter of 40-50 nm [44]. The particles are from transposons in yeast, which permit adaptation to extreme ISSN:2155-952X JBTBM an open access journal Silkworm Biotechnology J Biotechnol Biomaterial environments by giving opportunities for genetic modifications.…”

Section: Yeastmentioning

confidence: 99%

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Kato¹,

Deo²,

Park

et al. 2012

J Biotechnol Biomaterial

View full text Add to dashboard Cite

Virus-like particles (VLPs), which mimic the structure of authentic virus, are of significant importance owing to their wide ranging applications. VLPs have the potential to serve as candidate vaccine in addition to subunit vaccines and also serve as a nano-bioparticle scaffold for displaying complex foreign proteins. Here, we review different methods available to produce VLPs with various host expression systems including from microorganisms to mammalian cells and the current potential of silkworms as producers of these VLPs. Recently, silkworms have been used for efficient production of VLPs too in addition to mass production of recombinant proteins, which have contributed to molecular structural analysis, molecule-molecule interactions in biology and biotechnology.

show abstract

The yeast Ty virus-like particles

Cited by 55 publications

References 70 publications

BUD22 Affects Ty1 Retrotransposition and Ribosome Biogenesis in Saccharomyces cerevisiae

BUD22 Affects Ty1 Retrotransposition and Ribosome Biogenesis in Saccharomyces cerevisiae

T-body formation precedes virus-like particle maturation inS. cerevisiae

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Contact Info

Product

Resources

About