Worcester, B-chromosomes reached maximum frequencies at mid-altitudes in large populations, declining both towards the summit and in the small, isolated lowland populations. It is suggested that Bs in Crepis are excluded from populations growing under sub-optimal conditions. B-frequency in a population at East Wretham Heath, Norfolk, remained constant over three generations despite a very strong accumulation mechanism operating in C. capillaris. Selection must operate against individuals with one or more B-chromosomes to maintain this constancy.