“…Since their discovery in 1962, , argentation techniques have been one of the more important tools for lipid analysis, allowing the separation of different lipid classes based on the unsaturation degree of molecules. The technique is based on the chemical ability of silver ions to form weak charge-transfer complexes with electron-rich olefinic double bonds. , These complexes are stronger with cis than trans double bonds, allowing their separation by thin-layer (TLC) and high-performance liquid (HPLC) chromatography. , …”