Azimuthal fractalography of networks of biological crystals

Vanchulyak, Oleh; Ushenko, Yu. A.; Galochkin, O.V.; Sakhnovskiy, M. Yu.; Kovalchuk, M.; Dovgun, A.; Golub, S. Ya.; Dubolazov, Olexander V.; Sokolnyuk, S.; Litvinenko, O.; Bodnar, G. B.

doi:10.1117/12.2529337

Cited by 10 publications

(9 citation statements)

References 13 publications

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“…Methods of polarization tomography, based on the results of numerical display of instrumental measurement of parameters, are one of the most objective and accurate for recording changes that occurred in a biological object under the influence of environmental factors and changes in biological tissues (BT) during the pathological process. They also have the main requirement for the introduction of modern research methods in the practice of any expert field -the ability not to change the properties and condition of the object under study in its evaluation [3,19,20,21].…”

Section: Discussionmentioning

confidence: 99%

“…Recently in forensic practice optical methods of biological tissue research using lasers have positively proved themselves, which by studying the phenomena of light scattering make it possible to obtain reliable objective data of the dynamics of pre-and post-mortem changes of the studied tissues of the human body and to diagnose with greater accuracy prescription of pathological process in biological tissues and liquids [1,2,3,4,5,11,13,14,15,16,17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 99%

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Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

2020

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Differential diagnosis of the cause of death (CD) from ischemic cerebral infarction (ICI), hemorrhages of traumatic (HTG) and non-traumatic (HNG) genesis exclude the violent nature of death. The aim of our work was to develop forensic criteria for hemorrhage differentiation of traumatic and non-traumatic genesis and ICI by azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance (HBS). For the study were used native sections of HBS from 130 corpses in the case of: death from coronary heart disease – 40 of native sections (group 1 – control); HTG – 30 sections (group 2), ICI – 30 native sections (group 3), HNG – 30 native sections (group 4). Measuring the coordinate allocation meanings of parameters of polarization in the points of microscopic images was carried out at the location of the standard stokes-polarimeter. The effectiveness of intergroup differentiation of samples of deaths from traumatic hemorrhage and ischemic cerebral infarction reaches a satisfactory level and is 76-83%. Efficiency between group differentiation of samples of deaths from nontraumatic and traumatic hemorrhages reaches a satisfactory level and is 75-82%. As for differentiation between ischemic cerebral infarction and nontraumatic hemorrhages thise method is ineffective.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

2020

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“…Subsequently, the samples were sent to the laboratory of the Yuriy Fedkovych Institute of Physical, Technical and Computer Sciences, where they were investigated using the CDMA method. The application method is described in these sources (Vanchulyak, 2019;Ushenko, 2016;Bachinskiy, 2017). Results and discussion.…”

Section: Methodsmentioning

confidence: 99%

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Garazdiuk

Bachynskіy

Nechytailo

et al. 2020

PMP

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An issue that is often debated in forensic traumatology is the differential diagnosis of hemorrhages into the human brain substance (HBS) of traumatic and non-traumatic genesis. Objectives. This study aims to identify new criteria for objective forensic differentiation of hemorrhages of traumatic origin, cerebral infarction of ischemic and hemorrhagic genesis by using the method of complex degree of mutual anisotropy. For this study native sections of HBS from 125 corpses were used in the case of: death from coronary heart disease - 35 (28%) of native sections (Group 1 - control); hemorrhages of traumatic genesis - 30 (24%) sections (Group 2); ischemic cerebral infarction - 30 (24%) native sections(Group 3); and hemorrhages of non-traumatic genesis - 30 (24%) native sections (Group 4). Results. The statistical moments of the third and fourth orders, which characterize the asymmetry and excess of the complex degree of mutual anisotropy module size distributions, the strength of the method of polarization-correlation microscopy in the differentiation of the samples of the histological sections of the brain of control and experimental groups reached a good level — 87%-90%. Conclusion. The method of complex degree of mutual anisotropy allows differentiating with great precision the genesis of hemorrhage into the substance of the brain.

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“…The large degree of variation within existing post mortem assessment methods prompts researchers to find new approaches to solve this problem. Ushenko et al (2016); Garazdyuk et al (2016); Vanchulyak et al (2019) have shown the possibilities of medico-physical methods of investigating the relationship between necrotic changes in the structure of biological tissues of different morphological structure, as well as causes and prescription of death. Purpose The purpose of the study was to develop an objectively reliable forensic methodology to enhance the functionality of the high-precision definition of TSD over a long period of time according to polarization microscopic tomography of the polycrystalline structure of the VB of the human eye by statistical and wavelet analysis.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Possibilities of Analysis of the Map of Linear Birefringence of the Crystal Fraction of Vitreous Body for Accurate Determination of the Time Since Death

Sarkisova

Bachynskіy

Palamar

et al. 2020

PMP

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Introduction: This paper discusses the possibility of polarization microscopic tomography of polycrystalline structure of vitreous body (VB) for use in forensics and in determining the time since death (TSD). Objectives: The purpose of this study was to develop a new set of forensic criteria to enhance the functionality of the high-precision definition of TSD over a long period of time according to polarization microscopic tomography of the polycrystalline structure of the VB of the human eye by statistical and wavelet analysis. Results: We obtained the numerical values of the change in the magnitude of the statistical moments of the 1-4 orders, which characterize the coordinate distributions of the magnitude of the linear birefringence (LB) of the polycrystalline component of the VB by the magnitude of the TSD. The results from our research illustrate the differences between the optical anisotropy of fibrillar collagen networks of VB layers with different TSD. The sensitivity range (36 hours) and accuracy (15 minutes) of the method of polarization tomography of the LB distributions of the polycrystalline component of the VB layers in the determined TSD were established. Conclusion: These results confirm the experimental processes outlined accurately determine the time of death. In turn, will provide scientific evidence, specifications thereof, and objective expert opinion.

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Azimuthal fractalography of networks of biological crystals

Cited by 10 publications

References 13 publications

Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

Methods to Determine Complex Degrees of Mutual Anisotropy for the Differentiation of Hemorrhages in the Human Brain Substance Resulting From Traumatic and Non-Traumatic Genesis

Diagnostic Possibilities of Analysis of the Map of Linear Birefringence of the Crystal Fraction of Vitreous Body for Accurate Determination of the Time Since Death

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