Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees

Gómez, María Pilar Garcés; Segura, Juan

doi:10.1093/treephys/15.9.625

Cited by 18 publications

(34 citation statements)

References 19 publications

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“…Different types of light sources such as Growth-lux (GL) (Gómez and Segura 1994), high-pressure lamps (Scaltsoyiannes et al 1994) and their combinations such as fluorescent light supplemented with incandescent light (Flygh et al 1998) are also applied. Different light quality influenced the rooting frequencies according to the PPFD used.…”

Section: Lightmentioning

confidence: 99%

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Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.

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Section: Lightmentioning

confidence: 99%

“…The majority of studies relating to ARF applied light intensities that varied from 60 to 80 lmol m -2 s -1 (Gómez and Segura 1994;Scaltsoyiannes et al 1994;Nandwani et al 2001;Zhang et al 2006;Ishii et al 2007). The influence of light (quality) on ARF in shoots of other species is listed in Table 2.…”

Section: Lightmentioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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“…It is assumed that members of the Cupressaceae family have low cytokinin requirements (Negussie, 1997), and, thus, the formation of axillary shoots was evident in all cultures (Table 2). Micropropagation by axillary shoot multiplication is typical for Juniperus species and has been adopted by many researchers (Gómez and Segura, 1995;Loureiro et al, 2007;Castro et al, 2011;Zaidi et al, 2012). The procedures involved growing explants normally from adult trees on media with different concentrations of cytokinins and auxins.…”

Section: Effects Of Growth Regulators On In Vitro Morphogenesismentioning

confidence: 99%

“…Although these techniques were successful for in vitro regeneration, certain morphogenic responses, such as lack of leaf differentiation, were observed in some cultures when we conducted experiments utilizing plant material collected from different donor plants, locations and/or seasons (unpublished results). Loss of morphogenetic potential, formation of different morphotypes and/or explant hyperhydricity, observed in cultures of J. oxycedrus as well as J. excela and J.phoenicea, can be due to the explants' physiological and/or physical conditions, or attributed to the environment from where the plant material is collected (Gómez and Segura, 1995;Negussie, 1997;Loureiro et al, 2007). Therefore, further development of in vitro regeneration protocols of J. phoenicea is needed, not least for transformation studies, where selection pressure can reduce regeneration efficiency.…”

Section: Introductionmentioning

confidence: 99%

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Al-Ramamneh¹,

Daradkeh²,

Rababah³

et al. 2017

Aust J Crop Sci

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Juniperus phoenicea is an ornamental shrub that is also used to flavor food and to supply medicines and timber. Its micropropagation is of industrial concern and can occur by axillary shoot multiplication. Microcuttings of J. phoenicia were established in vitro on Murashige and Skoog (MS) and Rugini Olive (OM) media in glass tubes (25 mm x 150 mm). Factors studied were explant length (0.5 or 1.5 cm) and orientation (horizontal or vertical), media strength (OM, ½OM, MS, ½MS) and the following growth regulators: the anti-auxin 2,3,5-triiodobenzoic acid (TIBA), the cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ), and the growth retardant daminozide (DM). Microcuttings placed vertically on the surface of OM, ½OM and ½MS media without hormones exhibited axillary bud differentiation, but they were swollen and turned brown one month later when placed horizontally on the medium surface. The number of shoots, averaged across OM, ½OM and ½MS media, was significantly higher from longer (1.5 cm) than shorter (0.5 cm) microcuttings (4.11 versus 1.57 shoots microcutting -1 ) after 60 days of culture. TIBA or DM at 0.1 mg l -1 included in OM medium enhanced leaf differentiation, callus induction and formation of adventitious shoots over three months from 0.5 cm long microcuttings taken from in vitro shoots. The formation of adventitious shoots was sporadic and occurred at a rate of 1 shoot microcutting -1 in the presence of 0.1 mg l -1 DM. OM supplemented with 0.1 mg l -1 TIBA resulted in significantly the highest leaf differentiation (55 leaves microcutting -1 ), with a rooting rate of 40%. Contents of phenols, flavonoids and antioxidants were compared for cuttings from young seedlings, callus, in vitro shoots, and seeds. Antioxidant activity was significantly the highest for shoots grown on hormone-free OM medium and callus maintained on OM medium containing 0.1 mg l -1 2,4-D (94.7 and 94.3% inhibition of DPPH free radicals, respectively). Thus, different routes for in vitro regeneration of J. phoenicea can be of potential use for many biotechnological, pharmaceutical and food industry applications.

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“…In vitro micropropagation methods have been intensively used for the clonal propagation of many tree species, of which fruit and timber trees are of great eco-nomic importance (Gomez and Segura, 1995). However, many tree species are not suitable for vegetative multiplication and in those which are suitable, to a certain extent, the process is rather slow.…”

Section: Introductionmentioning

confidence: 99%