This research discussed the model with two turning points. Homoscedastic test conducted to see the model's predetermined regulation.Uniformity wasn't shown under brighter conditions, therefore goodness of fit test was conducted not only with normal distribution but also with logistic distribution. By adapting multinomial logistic analysis, the model's adaptation to case of unequal variance was shown. In addition, to adapt the experiment of adjustment method, goodness of fit with quartic function (two inflection points) was tested. This showed the possibility of deriving the true response by curve of response rate, and expanding the model's application range.