Avidity of IgG for<i>Streptococcus pneumoniae</i>Type 6B and 23F Polysaccharides in Infants Primed with Pneumococcal Conjugates and Boosted with Polysaccharide or Conjugate Vaccines

Anttila, Marja; Eskola, Juhani; Åhman, Heidi; Käyhty, Helena

doi:10.1086/515298

Cited by 130 publications

(87 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…on May 10, 2018 by guest http://iai.asm.org/ immune rabbits were quite high relative to the levels of antibody (i.e., potent), we characterized the progression of PAspecific avidity maturation in the sera of the second group of MAP-immune rabbits by using an NH 4 SCN chaotrope elution ELISA (3,31,41). In this ELISA, resistance to disruption of the antigen-antibody interaction in the presence of a fixed concentration of chaotrope is directly correlated with antibody avidity (12).…”

Section: Vol 77 2009 Synthetic Peptide Vaccine For Anthrax 3383mentioning

confidence: 99%

Synthetic Peptide Vaccine Targeting a Cryptic Neutralizing Epitope in Domain 2 ofBacillus anthracisProtective Antigen

Oscherwitz

Jacobs

et al. 2009

Infect Immun

View full text Add to dashboard Cite

Current evidence suggests that protective antigen (PA)-based anthrax vaccines may elicit a narrow neutralizing antibody repertoire, and this may represent a vulnerability with PA-based vaccines. In an effort to identify neutralizing specificities which may complement those prevalent in PA antiserum, we evaluated whether sequences within the 2␤2-2␤3 loop of PA, which are apparent in the crystal structure of heptameric but not monomeric PA, might represent a target for an epitope-specific vaccine for anthrax and, further, whether antibodies to these sequences are induced in rabbits immunized with monomeric PA. We evaluated the immunogenicity in rabbits of a multiple antigenic peptide (MAP) displaying copies of amino acids (aa) 305 to 319 of this region. Overall, four out of six rabbits vaccinated with the MAP peptide in Freund's adjuvant developed high-titer, high-avidity antibody responses which cross-reacted with the immobilized peptide sequence comprising aa 305 to 319 and with PA, as determined by an enzyme-linked immunosorbent assay, and which displayed potent and durable neutralization of lethal toxin (LeTx) in vitro, with peak titers which were 452%, 100%, 67%, and 41% of the peak neutralization titers observed in positive-control rabbits immunized with PA. Importantly, analysis of sera from multiple cohorts of rabbits with high-titer immunity to PA demonstrated a virtual absence of this potent antibody specificity, and work by others suggests that this specificity may be present at only low levels in primate PA antiserum. These results highlight the potential importance of this immunologically cryptic neutralizing epitope from PA as a target for alternative and adjunctive vaccines for anthrax.

show abstract

Section: Vol 77 2009 Synthetic Peptide Vaccine For Anthrax 3383mentioning

confidence: 99%

Synthetic Peptide Vaccine Targeting a Cryptic Neutralizing Epitope in Domain 2 ofBacillus anthracisProtective Antigen

Oscherwitz

Jacobs

et al. 2009

Infect Immun

View full text Add to dashboard Cite

show abstract

“…An EIA method described earlier by Anttila et al 31 was used to determine the relative avidity of Pnc PS IgG antibodies. Microtitre plates (MaxiSorp) were coated, as above, with Pnc PSs.…”

Section: Eia For Measuring Avidity Of Pnc Ps Igg Antibodiesmentioning

confidence: 99%

IgG subclasses and avidity of antibodies to polysaccharide antigens in allogeneic BMT recipients after vaccination with pneumococcal polysaccharide and Haemophilus influenzae type b conjugate vaccines

Parkkali

Käyhty

Anttila

et al. 1999

Bone Marrow Transplant

View full text Add to dashboard Cite

show abstract

“…The strainspecific antibody titer to the whole cell preparation of NTHi in plasma or BAL fluid was also determined by a previously described method, with minor modifications . Because of the limitations in measuring the avidity of the strain-specific antibody to the whole cell preparation of NTHi, IgA specific and its avidity to OMP in BAL fluid were determined by ELISA employing 0.5 M sodium thiocyanate (Anttila et al 1998). The OMPs from each NTHi strain were prepared as previously described (Kurita et al 2006).…”

Section: Determination Of P6 or Strain-specific Antibodiesmentioning

confidence: 99%

Strain-Specific Pulmonary Defense Achieved after Repeated Airway Immunizations with Non-Typeable Haemophilus Influenzae in a Mouse Model

Koyama

Ahmed

Zhao

et al. 2007

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Strain-specific immune responses may play a critical role in the acute exacerbation of chronic obstructive pulmonary disease (COPD) caused by Haemophilus influenzae (NTHi), and the outer membrane protein P2 is one of surface antigens of NTHi, which may contribute to the strain-specific protective immunity. We examined whether repeated airway immunizations with killed-NTHi strains bearing different P2 molecules were capable of inducing protective immunity against homologous or heterologous strains in the lungs of a mouse model. Three different strains of NTHi were used in this study. Three serial intratracheal (IT) immuizations of a single strain or three different strains of NTHi led to the production of cross-reactive immunoglobulins G and A in bronchoalveolar lavage fluids. Three serial IT immunizations with a single strain enhanced the bacterial clearance of the homologous strain in the lungs, but no enhancement of bacterial clearance was found with three serial IT immunizations of heterologous strains. The enhancement in bacterial clearance, therefore, appears to be primarily strain-specific. Enhanced bacterial clearance of a hetrologous strain was also found after three serial IT immunizations of a single strain among two of the three strains employed for bacterial challenge. These findings suggest that P2 molecules and surface antigens other than P2 are involved in the development of pulmonary defense against NTHi in mice. Our data may explain, in part, why patients with COPD experience recurrent NTHi infections.non-typeable Haemophilus influenzae; outer membrane protein P2; pulmonary defense; chronic obstructive pulmonary disease; acute exacerbation

show abstract

Avidity of IgG forStreptococcus pneumoniaeType 6B and 23F Polysaccharides in Infants Primed with Pneumococcal Conjugates and Boosted with Polysaccharide or Conjugate Vaccines

Cited by 130 publications

References 40 publications

Synthetic Peptide Vaccine Targeting a Cryptic Neutralizing Epitope in Domain 2 ofBacillus anthracisProtective Antigen

Synthetic Peptide Vaccine Targeting a Cryptic Neutralizing Epitope in Domain 2 ofBacillus anthracisProtective Antigen

IgG subclasses and avidity of antibodies to polysaccharide antigens in allogeneic BMT recipients after vaccination with pneumococcal polysaccharide and Haemophilus influenzae type b conjugate vaccines

Strain-Specific Pulmonary Defense Achieved after Repeated Airway Immunizations with Non-Typeable Haemophilus Influenzae in a Mouse Model

Contact Info

Product

Resources

About