Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle

Lavoria, María Ángeles; Digiacomo, Sebastian; Bucafusco, Danilo; Franco-Mahecha, Olga Lucía; Pérez-Filgueira, Daniel Mariano; Capozzo, Alejandra Victoria

doi:10.1016/j.vaccine.2012.09.011

Cited by 50 publications

(62 citation statements)

References 33 publications

(55 reference statements)

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“…In our study, when all of the naive subjects were considered together, the overall pattern of avidity was as expected, with increasing AI after each dose of vaccine. Our aggregate data are therefore similar to "normal" AI observations following live-attenuated vaccination (33)(34)(35) and studies following inactivated vaccines with or without adjuvants (30,(36)(37)(38). However, it is interesting that, in a study of antibody avidity to multiple doses of inactivated poliovirus vaccine in infants, the AI profile was essentially flat after the first two doses.…”

Section: Discussionsupporting

confidence: 79%

Unusual Patterns of IgG Avidity in Some Young Children following Two Doses of the Adjuvanted Pandemic H1N1 (2009) Influenza Virus Vaccine

Yam

Gupta

Brewer

et al. 2013

Clin Vaccine Immunol

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Section: Discussionsupporting

confidence: 79%

Unusual Patterns of IgG Avidity in Some Young Children following Two Doses of the Adjuvanted Pandemic H1N1 (2009) Influenza Virus Vaccine

Yam

Gupta

Brewer

et al. 2013

Clin Vaccine Immunol

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“…In our study the two mildly sick cattle that received the parental vaccine had log 10 neutralizing titres of 2.1 and 2.0 at 21 dpv and had detectable virus in the nasal cavity. Lavoria et al [47] showed that IgG isotypes in a heterologous FMD response may provide complementary information to the VNT assessment following vaccination. In our study we have measured the homologous anti-FMD response in vaccinated cattle and that both IgG1 and IgG2 increased following FMD vaccination.…”

Section: Discussionmentioning

confidence: 99%

“…This data suggests that there are antibody mediated protective mechanisms besides neutralization that become evident when neutralizing antibodies titres are low or borderline positive. This may be as a result of other components of the host immune system such as cell-mediated immune response, the complement system and phagocytosis [46] or different clearing mechanisms associated with the different immunoglobulin isotypes or the absence of high avidity antibodies [47,48] that were not accounted for in this study. We did not measured the antibody avidity or the role of cell-mediated response in this experiment and it is therefore not unreasonable to expect that the single immunization of cattle did not allow sufficient development of high avidity antibodies through affinity maturation or the development of optimal isotypes in some individual animals.…”

Section: Discussionmentioning

confidence: 99%

Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge

Maree

Nsamba

Mutowembwa

et al. 2015

Vaccine

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The genetic diversity of the three Southern African Territories (SAT) types of foot-and-mouth disease virus (FMDV) reflects high antigenic variation, and indications are that vaccines targeting each SATspecific topotype may be needed. This has serious implications for control of FMD using vaccines as well as the choice of strains to include in regional antigen banks. Here, we investigated an intra-serotype chimeric virus, vSAT2 ZIM14 -SAT2, which was engineered by replacing the surface-exposed capsid-coding region (1B-1D/2A) of a SAT2 genome-length clone, pSAT2, with that of the field isolate, SAT2/ZIM/14/90. The chimeric FMDV produced by this technique was viable, grew to high titres and stably maintained the 1B-1D/2A sequence upon passage. Chemically inactivated, oil adjuvanted vaccines of both the chimeric and parental immunogens were used to vaccinate cattle. The serological response to vaccination showed the production of strong neutralizing antibody titres that correlated with protection against homologous FMDV challenge. We also predicted a good likelihood that cattle vaccinated with an intra-serotype chimeric vaccine would be protected against challenge with viruses that caused recent outbreaks in southern Africa. These results provide support that chimeric vaccines containing the external capsid of field isolates induce protective immune responses in FMD host species similar to the parental vaccine.

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“…Previous studies have shown that there is a strong relationship between antibody response and protection (36)(37)(38). The precision of this relationship can be improved slightly by including other immunological techniques (39,40). However, the degree of the titer that relates with protection is not the same for different strains.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

Tekleghiorghis¹,

Weerdmeester²,

Hemert-Kluitenberg³

et al. 2014

Clin. Vaccine Immunol.

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Vaccination has been one of the most important interventions in disease prevention and control. The impact of vaccination largely depends on the quality and suitability of the chosen vaccine. To determine the suitability of a vaccine strain, antigenic matching is usually studied by in vitro analysis. In this study, we performed three in vitro test methods to determine which one gives the lowest variability and the highest discriminatory capacity. Binary ethylenimine inactivated vaccines, prepared from 10 different foot-and-mouth disease (FMD) virus serotype A strains, were used to vaccinate cattle (5 animals for each strain). The antibody titers in blood serum samples 3 weeks postvaccination (w.p.v.) were determined by a virus neutralization test, neutralization index test, and liquid-phase blocking enzyme-linked immunosorbent assay (ELISA). The titers were then used to calculate relationship coefficient (r 1 ) values. These r 1 values were compared to the genetic lineage using receiver operating characteristic (ROC) analysis. In the two neutralization test methods, the median titers observed against the test strains differed considerably, and the sera of the vaccinated animals did not always show the highest titers against their respective homologous virus strains. When the titers were corrected for test strain effect (scaling), the variability (standard error of the mean per vaccinated group) increased because the results were on a different scale, but the discriminatory capacity improved. An ROC analysis of the r 1 value calculated on both observed and scaled titers showed that only r 1 values of the liquid-phase blocking ELISA gave a consistent statistically significant result. Under the conditions of the present study, the liquid-phase blocking ELISA showed less variation and still had a higher discriminatory capacity than the other tests.

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Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle

Cited by 50 publications

References 33 publications

Unusual Patterns of IgG Avidity in Some Young Children following Two Doses of the Adjuvanted Pandemic H1N1 (2009) Influenza Virus Vaccine

Unusual Patterns of IgG Avidity in Some Young Children following Two Doses of the Adjuvanted Pandemic H1N1 (2009) Influenza Virus Vaccine

Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge

Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching

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