Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain

Matsuyama, Makoto; Ohashi, Yohei; Tsubota, Tadashi; Yaguchi, Masatsugu; Kato, Shigeki; Kobayashi, Kazuto; Miyashita, Yasushi

doi:10.1073/pnas.1423963112

Cited by 9 publications

(9 citation statements)

References 74 publications

(91 reference statements)

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“…The cloning of ASLV envelopes and their receptors has revealed their specificity (Barnard et al, 2006;Chai and Bates, 2006): TVB S1 for EnvB and EnvE, TVB S3 for EnvB, TVC for EnvC, and TVJ for EnvJ (Smith et al, 1998;Adkins et al, 2000;Elleder et al, 2005;Chai and Bates, 2006). By using these systems, lentiviral vectors pseudotyped with EnvB, EnvC, and EnvE have been reported to infect cells expressing their corresponding receptors (Matsuyama et al, 2015).…”

Section: Development Of a Chimeric Aslv/rv-g Envelope-pseudotyped Rv∆gmentioning

confidence: 99%

“…EnvD was excluded from this procedure because the EnvD-packaged virus may infect mammalian cells that lack foreign receptors (Bova et al, 1988). EnvJ was also omitted because TVJ, an EnvJ receptor, is a sodium-proton exchanger that may potentially affect neuronal activity (Chai and Bates, 2006;Matsuyama et al, 2015).…”

Section: Development Of a Chimeric Aslv/rv-g Envelope-pseudotyped Rv∆gmentioning

confidence: 99%

“…TVB reportedly has the following variants with different affinities for various ASLVs: TVB S1 , TVB r2 , TVB S3 , and TVB T (Brojatsch et al, 1996;Adkins et al, 1997Adkins et al, , 2000Smith et al, 1998). Among the several subtypes, we selected TVB S3 because it does not recognize and bind to EnvE, whereas the other TVB variants bind to both EnvB and EnvE (Adkins et al, 2000;Klucking and Young, 2004;Reinisová et al, 2008;Matsuyama et al, 2015). However, TVB is a homolog of the mammalian TNF-related apoptosis-inducing ligand death receptor 4/5; when ASLV-B and ASLV-E infected cells via TVB, TVB induced apoptosis (Brojatsch et al, 1996(Brojatsch et al, , 2000Smith et al, 1998;Klucking et al, 2005).…”

Section: Generation Of the Optimized Oenvb/otvb Systemmentioning

confidence: 99%

“…Each column represents the mean ± SEM (n = 3). * P < 0.05, * * * P < 0.001 vs. because several lines of evidence have indicated that TVB S1 and TVB T have affinities to ASLV-B and ASLV-E (Brojatsch et al, 2000;Klucking and Young, 2004;Matsuyama et al, 2015). We counted the number of infected cells 3 days after the infection.…”

Section: Generation Of the Optimized Oenvb/otvb Systemmentioning

confidence: 99%

“…We next aimed to develop optimized DR46-TVB receptors that would allow specific targeting of EnvE viruses in the mammalian brain (Klucking and Young, 2004;Matsuyama et al, 2015). Intriguingly, DR46-TVB has been developed as an EnvE-specific receptor by replacing the TVB S1 CRD1 region with a corresponding region of death receptor 5 (Klucking and Young, 2004).…”

Section: Generation Of the Optimized Oenve/otve Systemmentioning

confidence: 99%

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Multiplex Neural Circuit Tracing With G-Deleted Rabies Viral Vectors

Suzuki

Morimoto

Akaike

et al. 2020

Front. Neural Circuits

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Neural circuits interconnect to organize large-scale networks that generate perception, cognition, memory, and behavior. Information in the nervous system is processed both through parallel, independent circuits and through intermixing circuits. Analyzing the interaction between circuits is particularly indispensable for elucidating how the brain functions. Monosynaptic circuit tracing with glycoprotein (G) gene-deleted rabies viral vectors (RV∆G) comprises a powerful approach for studying the structure and function of neural circuits. Pseudotyping of RV∆G with the foreign envelope EnvA permits expression of transgenes such as fluorescent proteins, genetically-encoded sensors, or optogenetic tools in cells expressing TVA, a cognate receptor for EnvA. Transcomplementation with rabies virus glycoproteins (RV-G) enables trans-synaptic labeling of input neurons directly connected to the starter neurons expressing both TVA and RV-G. However, it remains challenging to simultaneously map neuronal connections from multiple cell populations and their interactions between intermixing circuits solely with the EnvA/TVA-mediated RV tracing system in a single animal. To overcome this limitation, here, we multiplexed RV∆G circuit tracing by optimizing distinct viral envelopes (oEnvX) and their corresponding receptors (oTVX). Based on the EnvB/TVB and EnvE/DR46-TVB systems derived from the avian sarcoma leukosis virus (ASLV), we developed optimized TVB receptors with lower or higher affinity (oTVB-L or oTVB-H) and the chimeric envelope oEnvB, as well as an optimized TVE receptor with higher affinity (oTVE-H) and its chimeric envelope oEnvE. We demonstrated independence of RV∆G infection between the oEnvA/oTVA, oEnvB/oTVB, and oEnvE/oTVE systems and in vivo proof-of-concept for multiplex circuit tracing from two distinct classes of layer 5 neurons targeting either other cortical or subcortical areas. We also successfully labeled common input of the lateral geniculate nucleus to both cortico-cortical layer 5 neurons and inhibitory neurons of the mouse V1 with multiplex RV∆G tracing. These oEnvA/oTVA, oEnvB/oTVB, and oEnvE/oTVE systems allow for differential labeling of distinct circuits to uncover the mechanisms underlying parallel processing through independent circuits and integrated processing through interaction between circuits in the brain.

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Section: Development Of a Chimeric Aslv/rv-g Envelope-pseudotyped Rv∆gmentioning

confidence: 99%

Section: Development Of a Chimeric Aslv/rv-g Envelope-pseudotyped Rv∆gmentioning

confidence: 99%

Section: Generation Of the Optimized Oenvb/otvb Systemmentioning

confidence: 99%

Section: Generation Of the Optimized Oenvb/otvb Systemmentioning

confidence: 99%

Section: Generation Of the Optimized Oenve/otve Systemmentioning

confidence: 99%

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Multiplex Neural Circuit Tracing With G-Deleted Rabies Viral Vectors

Suzuki

Morimoto

Akaike

et al. 2020

Front. Neural Circuits

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Recombinant Viral Vectors as Neuroscience Tools

et al. 2019

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Recombinant viruses are highly efficient vehicles for in vivo gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans-synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno-associated viruses, retro/lentiviruses, and glycoprotein-deleted rabies viruses. C 2019 by John Wiley & Sons, Inc.Keywords: AAV r gene delivery r lentivirus r rabies delta-G r retrovirus r virus How to cite this article:

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Exploring propriospinal neuron-mediated neural circuit plasticity using recombinant viruses after spinal cord injury

Deng

Ravenscraft

2022

Experimental Neurology

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Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain

Cited by 9 publications

References 74 publications

Multiplex Neural Circuit Tracing With G-Deleted Rabies Viral Vectors

Multiplex Neural Circuit Tracing With G-Deleted Rabies Viral Vectors

Recombinant Viral Vectors as Neuroscience Tools

Exploring propriospinal neuron-mediated neural circuit plasticity using recombinant viruses after spinal cord injury

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