Exploring propriospinal neuron-mediated neural circuit plasticity using recombinant viruses after spinal cord injury

Deng, Ling-Xiao; Ravenscraft, Baylen; Xu, Xiao–Ming

doi:10.1016/j.expneurol.2021.113962

Cited by 3 publications

(3 citation statements)

References 82 publications

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“…Despite these advancements, few studies have documented brain circuits activities during seizures through recombinant adenoviral methodologies. Currently, lentiviruses and rabies viruses are widely used in the tracing of neural circuits (Kato and Kobayashi 2020 ; Deng et al 2022 ). It is crucial to choose different viral vectors based on specific research objectives.…”

Section: Virus Tracing Technologymentioning

confidence: 99%

Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research

Xiao,

Li,

Kong

et al. 2024

Cell Mol Neurobiol

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Epilepsy, a prevalent neurological disorder characterized by high morbidity, frequent recurrence, and potential drug resistance, profoundly affects millions of people globally. Understanding the microscopic mechanisms underlying seizures is crucial for effective epilepsy treatment, and a thorough understanding of the intricate neural circuits underlying epilepsy is vital for the development of targeted therapies and the enhancement of clinical outcomes. This review begins with an exploration of the historical evolution of techniques used in studying neural circuits related to epilepsy. It then provides an extensive overview of diverse techniques employed in this domain, discussing their fundamental principles, strengths, limitations, as well as their application. Additionally, the synthesis of multiple techniques to unveil the complexity of neural circuits is summarized. Finally, this review also presents targeted drug therapies associated with epileptic neural circuits. By providing a critical assessment of methodologies used in the study of epileptic neural circuits, this review seeks to enhance the understanding of these techniques, stimulate innovative approaches for unraveling epilepsy's complexities, and ultimately facilitate improved treatment and clinical translation for epilepsy. Graphical Abstract

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Section: Virus Tracing Technologymentioning

confidence: 99%

Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research

Xiao,

Li,

Kong

et al. 2024

Cell Mol Neurobiol

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“…However, LUT MNs do not receive direct commands from the PMC or sensory input from the BL. Instead, these commands and inputs are modulated through a cascade of local and propriospinal interneurons (INs) (Marson et al 2003;Karnup 2021;Karnup and De Groat 2020a;Zholudeva et al 2021;Deng, Ravenscraft, and Xu 2022). Thus, well-tuned ensembles of spinal INs play a critical role in coordination of the EUS and BL muscles, allowing efficient elimination of accumulated urine, although this is often incomplete even in SI animals (Yoshimura et al 2023).…”

Section: Introductionmentioning

confidence: 99%

“…2003; Karnup 2021; Karnup and De Groat 2020a; Zholudeva et al. 2021; Deng, Ravenscraft, and Xu 2022). Thus, well‐tuned ensembles of spinal INs play a critical role in coordination of the EUS and BL muscles, allowing efficient elimination of accumulated urine, although this is often incomplete even in SI animals (Yoshimura et al.…”

Section: Introductionmentioning

confidence: 99%

Sexual Dimorphism of Spinal Neural Circuits Controlling the Mouse External Urethral Sphincter With and Without Spinal Cord Injury

Karnup,

Hashimoto,

Cho

et al. 2024

J of Comparative Neurology

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Spinal cord injury (SCI) disrupts coordination between the bladder and the external urinary sphincter (EUS), leading to transient or permanent voiding impairment, which is more severe in males. Male versus female differences in spinal circuits related to the EUS as well as post‐SCI rewiring are essential for understanding of sex‐/gender‐specific impairments and possible recovery mechanisms. To quantitatively assess differences between EUS circuits in males versus females and in spinal intact (SI) versus SCI animals, we retrogradely traced and counted EUS‐related neurons. In transgenic ChAT‐GFP mice, motoneurons (MNs), interneurons (INs), and propriospinal neurons (PPNs) were retrogradely trans‐synaptically traced with PRV614‐red fluorescent protein (RFP) injected into EUS. EUS‐MNs in dorsolateral nucleus (DLN) were separated from other GFP+ MNs by tracing them with FluoroGold (FG). We found two morphologically distinct cell types in DLN: FG+ spindle‐shaped bipolar (SB‐MNs) and FG− rounded multipolar (RM‐MNs) cholinergic cells. Number of MNs of both types in males was twice as large as in females. SCI caused a partial loss of MNs in all spinal nuclei. After SCI, males showed a fourfold rise in the number of RFP‐labeled cells in retro‐DLN (RDLN) innervating hind limbs. This suggests (a) an existence of direct synaptic interactions between spinal nuclei and (b) a post‐SCI increase of non‐specific inputs to EUS‐MNs from other motor nuclei. Number of INs and PPNs deferred between males and females: In SI males, the numbers of INs and PPNs were ∼10 times larger than in SI females. SCI caused a twofold decrease of INs and PPNs in males but not in females.

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Proper wiring of newborn neurons to control bladder function after complete spinal cord injury

et al. 2023

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Exploring propriospinal neuron-mediated neural circuit plasticity using recombinant viruses after spinal cord injury

Cited by 3 publications

References 82 publications

Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research

Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research

Sexual Dimorphism of Spinal Neural Circuits Controlling the Mouse External Urethral Sphincter With and Without Spinal Cord Injury

Proper wiring of newborn neurons to control bladder function after complete spinal cord injury

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