Avian Salmonella Infection: Isolation and Identification of Organisms and Histopathological Study

Abstract

The Present study was performed in the Department of Pathology, BAU, Mymensingh during the period from January to December 2004. The study was conducted to determine the occurrence and pathology of pullorum disease, fowl typhoid and salmonellosis (paratyphoid infection) in dead chickens at necropsy in Mymensingh district of Bangladesh. A total of 70 samples (liver, ovary and intestine) were collected for isolation of bacteria in different media, and identification was performed based on the staining, cultural and biochemical properties of Salmonella spp. Routine histopathological method was used for the detection of tissue level alterations in Salmonella infected cases. Grossly, in all the infected cases the liver was enlarged and congested and in few cases, liver discoloration with focal necrosis. Old raised hemorrhages in the caecal tonsil and congested deformed ova were other important findings. There was catarrhal inflammation in the intestine. Microscopically, the section of livers showed congestion, hemorrhages, focal necrosis with infiltration of mononuclear cells. The pulmonary lesions consisted of sero-fibrinous exudation with mononuclear cell infiltration. The intestinal mucosa exhibited congestion, hemorrhages and infiltration of plasma cells, heterophils and macrophages. . Out of 70 samples, 8 isolates were identified as Salmonella (11.42 %). Of them, five isolates were identified as Salmonella gallinarum, causative agent of fowl typhoid, one isolate was characterized as Salmonella pullorum, causative agent of pullorum disease and other two motile salmonella were identified as paratyphoid infection.

Seroprevalence of salmonellosis in layer chickens with isolation, identification and antibiogram study of their causal agents

“…It indicated that the 12 (80%) isolates were Salmonella gallinarum and 3 (20%) were Salmonella pullorum. The higher rate of Salmonella gallinarum than S. pullorum is supported by Hossain et al (2006) who detected 62.5% S. gallinarum and 25% S. pullorum.…”

“…The prevalence was varied in terms of age (Table 1) Isolation of causal agent A total of 15 (7.5%) Salmonellae were isolated from 200 liver of dead birds of selected flocks of different groups and characterized by using specific biochemical tests and Gram's staining technique ( Table 2). The rate of isolation was slightly lower than Hossain et al (2006) but markedly lower than Islam et al (2006). This may be due to variation of severity.…”

Clinical signs in Japanese quails (Coturnix coturnix) experimentally infected with Salmonella Gallinarum

“…Contudo, quando a replicação não é controlada, a salmonela se replica no baço e fígado acarretando a uma acentuada hepatoesplenomegalia (Chappell et al, 2009). Estudos anteriores apontaram que as alterações macroscópicas do tifo aviário são caracterizadas por aumento do fígado e do baço com áreas de necrose nesses órgãos (Hossain et al, 2006;Ezema;Onuoha;Chah, 2009) e hemorragia no ovário e nas tonsilas cecais (Hossain et al, 2006).…”

Pathomorphological, immunohistochemical and bacteriological findings in budgerigars (Melopsittacus undulatus) naturally infected withS.Gallinarum

“…The disease usually follows the ingestion of food or water contaminated by the excreta of clinically infected birds or carriers but can also be transmitted by attendants through contaminated hands, feet and clothes (Lowry et al, 1999;Berchieri et al, 2001). In the subacute and chronic stages of the disease, the liver and spleen are mostly affected, with multiple white foci, severe swelling and discolouration being observed (Hossain et al, 2006;Shivaprasad & Barrow, 2008).…”