Salmonellosis is a common problem in poultry farms of our country. Indiscriminate use of antibiotic to control the disease results drug resistance and limits the therapeutic possibilities in the treatment of the disease. This study was conducted during the period from January to May 2006 at Gobindapur of Dinajpur district. The present study was undertaken to determine the seroprevalence of salmonellosis in layer flocks and antibiogram study following isolation of Salmonellae. A total of 225 Star cross 579 brown chickens were studied with rapid serum plate agglutination test. Liver of 200 dead birds was studied for isolation and identification of Salmonellae. In vitro antibiotic sensitivity test of isolated Salmonellae was performed with commercial sensitivity discs. The overall seroprevalence was recorded 23.11%. The prevalence was varied from age to age. The highest rate was 28% in above 20 weeks of age. The antibiogram study revealed that the isolates were sensitive to ciprofloxacin (80%), nitrofurantoin (100%), sulphamethoxazole/ trimeoprim and amoxycillin (50%), tetracycline (60%) but resistant to penicillin-G and erythromycin. Further studies should be conducted on serotyping of the isolated Salmonellae, isolation and identification of Salmonellae from different feed and environmental sample.
The effectiveness of commercially available disinfectants was evaluated against Salmonellae isolated from different internal organs of 52 numbers of dead layer chickens in the district of Dinajpur during the period from July 2009 to June 2010. Bacterio-biochemical methods were used to isolate and identify the Salmonella organisms from 154 samples of liver, spleen, heart and lungs of birds, of which 36 (23.38%) samples had Salmonella infection. Organ-wise prevalence of Salmonella infection showed highest prevalence in liver ( 34.62%), followed by spleen (23.087%) , heart (20.00%) and lowest in lungs (4.00%). Effectiveness of disinfectants was tested at different concentration on Salmonella culture on SS agar media and measured by zone of inhibition incubated for 24 hours. Highest average zone of inhibition was recorded with Desinkap® (12.50 ± 2.08), followed by GPC (8) TM ® (11.33 ± 1.53) , and more or less similar patterns with TH 4 ® ( 10.50 ± 0.71), Virocid ® ( 10.50 ± 0.71) and Lysol ® ( 10.00 ± 0.00). It may be concluded from these results that Desinkap® is the most effective disinfectant against Salmonellae as it contains multiple ingredients.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11211 Bangl. J. Vet. Med. (2011). 9(1): 43-52
Foot and mouth disease (FMD) is a devastating viral disease and endemic in nature in Bangladesh that causes huge economical losses. The present research work was aimed to determine the prevalence of FMD outbreaks and molecular detection of FMDV serotypes by uRT-PCR and gsRT-PCR test, respectively from crossbred cattle in the Rajbari district of Bangladesh during the period from January to June 2018. A total of 16 tongue epithelial samples were collected from clinically FMD suspected 2 to 3 years old crossbred cattle. 14 samples were positive by uRT-PCR. The detection rate of FMDV by uRT-PCR was 87.50%. Then uRT-PCR positive samples were serotype by gsRT-PCR. Serotype based prevalence of FMDV was 42.86%, 100%, 21.43% and 21.43% in O serotype, Asia-1 serotype, A serotype and mixed infection with Asia-1 and A, respectively. Considering the age, the prevalence of confirmed FMD outbreak was 42.86%, 35.71% and 21.43% at the age of 2, 2.5 and 3 years, respectively. Serotype A, O and Asia-1 is circulated in Rajbari district and required trivalent vaccine for prevention and control of FMD in that area.
SAARC J. Agric., 19(1): 201-210 (2021)
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