Avian retroviral vectors derived from avian defective leukemia virus: Role of the translational context of the inserted gene on efficiency of the vectors

Benchaibi, M; Mallet, François; Thoraval, P.; Savatier, Pierre; Xiao, Jiumei; Verdier, Gérard; Samarut, Jacques; Nigon, Victor

doi:10.1016/0042-6822(89)90036-6

Cited by 32 publications

(29 citation statements)

References 37 publications

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“…Crosscoupling between ErbB and ER signaling pathways has been reported in rodent uterine tissues (IgnarTrowbridge et al, 1992) and is reminiscent of the cooperativity between the viral erbA and erbB oncogenes in the malignant transformation of avian hematopoietic cells (Benchaibi et al, 1989). In breast cancer, an inverse relation between ER and EGFR is quite clear.…”

Section: Introductionmentioning

confidence: 83%

Heregulin-β1 regulates the estrogen receptor-α gene expression and activity via the ErbB2/PI 3-K/Akt pathway

et al. 2003

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This study examines whether the serine/threonine protein kinase, Akt, is involved in the crosstalk between the ErbB2 and estrogen receptor-a (ER-a) pathways. Treatment of MCF-7 cells with 10 À9 m heregulin-b1 (HRG-b1) resulted in a rapid phosphorylation of Akt and a 15-fold increase in Akt activity. Akt phosphorylation was blocked by inhibitors of phosphatidylinositol 3-kinase (PI 3-K), by antiestrogens, the protein tyrosine kinase inhibitor, genistein, and by AG825, a selective ErbB2 inhibitor; but not by AG30, a selective EGFR inhibitor. Akt phosphorylation by HRG-b1 was abrogated by an arginine to cysteine mutation (R25C) in the pleckstrin homology (PH) domain of Akt, and HRG-b1 did not induce Akt phosphorylation in the ER-negative variant of MCF-7, MCF-7/ADR. Transient transfection of ER-a into these cells restored Akt phosphorylation by HRG-b1, suggesting the requirement of ER-a. HRG-b1 did not activate Akt in MCF-7 cells stably transfected with an anti-ErbB2-targeted ribozyme, further confirming a role for ErbB2. Stable transfection of the cells with a dominant negative Akt or with the R25C-Akt mutant, as well as PI 3-K inhibitors, blocked the effect of HRG-b1 on ER-a expression and activity and on the growth of MCF-7 cells. Stable transfection of MCF-7 cells with a constitutively active Akt mimicked the effect of HRG-b1. Experiments employing selective ErbB inhibitors demonstrate that the effect of HRG-b1 on ER-a expression and activity is also mediated by ErbB2 and not by EGFR, demonstrating that ErbB2 is the primary mediator of the effects of HRG-b1 on ER-a regulation. Taken together, our data suggest that HRG-b1, bound to the ErbB2 ErbB3 heterodimer, in the presence of membrane ER-a, interacts with and activates PI 3-K/Akt. Akt leads to nuclear ER-a phosphorylation, thereby altering its expression and transcriptional activity.

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Section: Introductionmentioning

confidence: 83%

Heregulin-β1 regulates the estrogen receptor-α gene expression and activity via the ErbB2/PI 3-K/Akt pathway

et al. 2003

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“…which is able to infect avian ceils only). We have previously described the generation of ALV-based vectors (Benchaibi et al, 1989;Cosset et al, 1991) and of the corresponding helper cell lines Cosset et al, 1990;. These helper-free retrovirus vectors have been used to transfer genes in early chick embryos (Thomas et al, 1992) and in primordial germ cells (Vick et al, 1993).…”

Section: -8819 (~) 1995 Chapman and Hall 1994mentioning

confidence: 97%

Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors

Thoraval

Afanassieff

Cosset

et al. 1995

Transgenic Research

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We have used vectors derived from avian leukosis viruses to transduce exogenous genes into early somatic stem cells of chicken embryos. The ecotropic helper cell line, Isolde, was used to generate stocks of NL-B vector carrying the Neo(r) selectable marker and the Escherichia coli lacZ gene. Microinjection of the NL-B vector directly beneath unincubated chicken embryo blastoderms resulted in infection of germline stem cells. One of the 16 male birds hatched (6.25%) from the injected embryos contained vector DNA sequences in its semen. Vector sequences were transmitted to G1 progeny at a frequency of 2.7%. Neo(r) and lacZ genes were transcribed in vitro in chicken embryo fibroblast cultures from transgenic embryos of the G2 progeny.

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“…Control myoblasts were infected by the TXN virus, derived from XJ12 by deleting the v-erb A sequence (Benchaibi et al, 1989). Myoblast infections were performed as previously described (Cassar-Malek et al, 1994).…”

Section: Cell Culturesmentioning

confidence: 99%

Changes in mitochondrial activity during avian myoblast differentiation: Influence of triiodothyronine or v-erbA expression

Rochard¹,

Cassar-Malek

Marchal

et al. 1996

J. Cell. Physiol.

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Numerous data suggest that mitochondrial activity is involved in the regulation of cell growth and differentiation. Therefore, we have studied the changes in mitochondrial activity in avian myoblast cultures (QM7 line) undergoing differentiation or in BrdU-treated, differentiation-deficient cells. As we have previously shown that triiodothyronine and v-erb A expression stimulate myogenic differentiation, we have also observed their influence upon mitochondrial activity. Comparison of control and BrdU-treated myoblasts indicated that precocious differentiation events were associated with a stimulation of citrate synthase and cytochrome oxidase activities. They also induced a transient decrease in mitochondrial membrane potential assessed by rhodamine 123 uptake. In control myoblasts, a general stimulation of mitochondrial activity was recorded at cell confluence, prior to terminal differentiation. These events did not occur in BrdU-treated myoblasts, thus indicating that they were tightly linked to myoblast commitment. Whereas no significant triiodothyronine influence could be detected upon mitochondrial activity, we observed that v-erb A expression significantly depresses the mitochondrial membrane potential in control myoblasts. This action was not observed in BrdU-treated myoblasts, thus suggesting that it involves an indirect pathway linked to differentiation. Moreover, the oncoprotein abrogated the decrease in E2-PDH subunit level observed at cell confluence. These data underline that changes in mitochondrial activity occurred prior to myoblast terminal differentiation and could be involved in the processes regulating myogenesis. In addition, they provide the first evidence that the v-erb A oncoprotein influences mitochondrial activity.

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Avian retroviral vectors derived from avian defective leukemia virus: Role of the translational context of the inserted gene on efficiency of the vectors

Cited by 32 publications

References 37 publications

Heregulin-β1 regulates the estrogen receptor-α gene expression and activity via the ErbB2/PI 3-K/Akt pathway

Heregulin-β1 regulates the estrogen receptor-α gene expression and activity via the ErbB2/PI 3-K/Akt pathway

Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors

Changes in mitochondrial activity during avian myoblast differentiation: Influence of triiodothyronine or v-erbA expression

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