Avian reticuloendotheliosis virus (strain T): V. DNA polymerase

Peterson, David A.; Baxter-Gabbard, K. L.; Levine, Alvin S.

doi:10.1016/0042-6822(72)90259-0

Cited by 35 publications

(19 citation statements)

References 10 publications

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“…In Southern blots with either EcoRI-PstIor HindIll-digested genomic DNA of P.polvcephaluin this fragment hybridized to a 3.5 or 5 kb band, respectively (see Figure 1). Genomic DNA was cut with these restriction enzymes, size selected by sucrose gradient density centrifugation (Peterson et al, 1972) and the corresponding fractions were cloned into pUCl9 and transformed into Ecoli JM83. The size-selected genomic libraries were screened with the 230 bp fragment, and two overlapping genomic clones with sizes of 3.5 and 5 kb were obtained.…”

Section: Methodsmentioning

confidence: 99%

A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.

et al. 1996

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Actin‐fragmin kinase (AFK) from Physarum polycephalum specifically phosphorylates actin in the EGTA‐resistant 1:1 actin‐fragmin complex. The cDNA deduced amino acid sequence reveals two major domains of approximately 35 kDa each that are separated by a hinge‐like proline/serine‐rich segment of 50 residues. Whereas the N‐terminal domain does not show any significant similarity to protein sequences from databases, there are six complete kelch repeats in the protein that comprise almost the entire C‐terminal half of the molecule. To prove the intrinsic phosphorylation activity of AFK, full‐length or partial cDNA fragments were expressed both in a reticulocyte lysate and in Escherichia coli. In both expression systems, we obtained specific actin phosphorylation and located the catalytic domain in the N‐terminal half. Interestingly, this region did not contain any of the known protein kinase consensus sequences. The only known sequence motif present that could have been involved in nucleotide binding was a nearly perfect phosphate binding loop (P‐loop). However, introduction of two different point mutations into this putative P‐loop sequence did not alter the catalytic activity of the kinase, which indicates an as yet unknown mechanism for phosphate transfer. Our data suggest that AFK belongs to a new class of protein kinases and that this actin phosphorylation might be the first example of a widely distributed novel type of regulation of the actin cytoskeleton in non‐muscle cells.

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Section: Methodsmentioning

confidence: 99%

A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.

et al. 1996

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“…However, in contrast to the ALSV group, REVs do not appear to be related to any endogenous type C retrovirus information in avian hosts (21). Moreover, REVs are quite distinct serologically, show little or no nucleic acid homology with members of the ALSV group, and neither genetically complement nor interfere with members of the ALSV group (12,20,22,23,32,38,48). Several studies on the morphology, major structural polypeptides, and DNA polymerase of REVs, presumably focusing on associated helper virus, have suggested that they are more closely related to type C viruses of mammalian origin than to the ALSV group (1,2,18,25,27,51).…”

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confidence: 98%

Low-molecular-weight RNAs and initiation of RNA-directed DNA synthesis in avian reticuloendotheliosis virus

Peters¹,

Glover²

1980

J Virol

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The small RNAs of avian reticuloendotheliosis virus (REV) were analyzed by two-dimensional polyacrylamide gel electrophoresis and compared with those of murine leukemia virus and avian sarcoma virus. Although there were some similarities among the three virus types, the patterns of small RNAs were distinct. By characterizing the small RNA which is most tightly associated with REV genome RNA and which can be labeled in limited DNA synthesis reactions, the primer for REV reverse transcription was identified as tRNAPrO. This is consistent with previous reports that REV is more closely related to retroviruses of mammalian origin than to other avian viruses. In contrast, REV strong-stop complementary DNA is longer than any previously characterized strong-stop products of avian or mammalian retroviruses. The REV group may, therefore, have been derived from an as yet unidentified mammalian type C virus.Reticuloendotheliosis virus (REV) is the prototype of a group of related, avian, type C retroviruses which cause a variety of lesions in infected birds (for a review, see reference 34).

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“…Preincubation of disrupted virus preparations with RNase significantly decreased the incorporation of [3H]TTP, indicating that the template is RNA, and the product of the polymerase assay was sensitive to DNase but not to RNase. The enzyme thus shares some of the characteristics attributed to polymerases from various other C-type viruses (14)(15)(16). No RDRP activity could be detected in association with the virus.…”

mentioning

confidence: 80%

“…The endogenous polymerase assay was modified from the method of Spiegelman et al (8). A standard incubation mixture of 0.1 ml contained: 0.2 mmole each dATP, dCTP, and dGTP; 0.109 pmole [3H]TTP (15)(16)(17)(18); 2.8 mmole 2-ME; 12 mmole MgCl,; 40 mmole KCl; and 57 mmole Tris-HC1 (pH 8.3). Virus suspended in Tris-HC1 (pH 8.3) was preincubated for 10 min at 0" with 0.08% Nonidet P-40 and 0.4% 2-ME.…”

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confidence: 99%

Biochemical Characteristics of Rat C-Type Virus WF-1

Bronson

Bookout

Bergs

et al. 1976

Experimental Biology and Medicine

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The discovery and morphological-immunological properties of the rat C-type virus WF-1, infectious for normal rat embryo cell cultures, have been previously described (1 , 2). This presumably endogenous C-type virus was spontaneously released by a cell line (WF-1) derived from normal Wistar-Furth rat embryos. The virus was also shown to be antigenically related to two C-type viruses (RMTDV and R-35) produced by cell lines derived from rat mammary tumors (3).Although all three viruses are similar morphologically and antigenically, only RMTDV had been found to produce some leukemogenic effects when inoculated into rats (4).It was therefore of interest to determine the pertinent biochemical characteristics of the WF-1 virus, non-oncogenic for rats, and to compare them with those of known oncogenic RNA viruses of other animal species. The results of this study are presented in this report. Materials and methods. Reagents. TNE buffer (0.01 M Tris, 0.1 M NaCl, and 0.001 M EDTA, pH 7.2), TNM buffer (0.01 M Tris, 0.1 M NaCl, and 0.001 M MgCI2), and sucrose solutions prepared in TNE buffer were sterilized by filtration (0.45-pm porosity, Millipore Corp., Bedford, Mass .) . Ribonuclease-free sucrose, [3H]uridine (sp act 25 to 28 Ci/mmole), [14C]uridine (sp act

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Avian reticuloendotheliosis virus (strain T): V. DNA polymerase

Cited by 35 publications

References 10 publications

A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.

A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.

Low-molecular-weight RNAs and initiation of RNA-directed DNA synthesis in avian reticuloendotheliosis virus

Biochemical Characteristics of Rat C-Type Virus WF-1

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