Avian pathogenic Escherichia coli - methods for improved diagnosis

Filho, Hiran Castagnino Kunert; Carvalho, Daiane; Grassotti, Tiela Trapp; Soares, Beatriz Dugaich; Rossato, Juliana Marzari; Cunha, Augusto César da; Brito, Kelly Cristina Tagliari de; Cavalli, Lissandra Souto; Brito, Benito Guimarães de

doi:10.1017/s0043933915000264

Cited by 8 publications

(4 citation statements)

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“…Hence, the European Union has enforced food safety legislation, which usually constitutes a blueprint for the bill in third countries [8,9]. For determining the virulence of E. coli strains, its inoculation into embryos or 1-day-old chicks is followed as a golden standard test [10], whereas serotyping remains the most frequently used diagnostic method in laboratories, although it only allows the identification of a limited number of avian pathogenic E. coli (APEC) strains [11]. Moreover, the prevalence of a certain serotype is linked with the geographical localization of a flock [3]; hence, various molecular typing methods have been employed to study APEC, but none has revealed a specific genotype [12].…”

Section: Introductionmentioning

confidence: 99%

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

et al. 2020

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A total of 54 broiler flocks during the first two weeks of life was used to investigate the incidence of avian pathogenic E. coli in Egypt; 28 isolates (51.85%) were revealed by colony morphology and biochemical identification which then investigated for their serogroups and only 18/28 isolates were serotyped. The most prevalent serotypes were O115, O142, O158, O55, O125, O114, O27, O20, and O15. By application of polymerase chain reaction (PCR), 83.3% (15/18) of the serotyped isolates were confirmed to be E. coli, and 93.3% (14/15), 46.6% (7/15), and 20% (3/15) of isolates harbored the iss, iutA, and fimH genes, respectively. Virulence testing of the selected 13 APEC isolates on the specific-pathogen-free (SPF) chicks revealed them to be highly virulent (15.4%), moderately virulent (23.1%), and avirulent (61.5%); however, all isolates (100%) were extremely virulent towards SPF embryonated chicken eggs. Antibiotic resistance (100% of isolates (n = 13)) was observed for ampicillin, amoxycillin–clavulanic acid, and tetracyclines, colistin (92.31%; 12/13), doxycycline and spiramycin (84.62%; 11/13), florfenicol (69.23%; 9/13), cefotaxime (61.54%; 8/13), and ciprofloxacin (53.85%; 7/13). The highest percentage of sensitivity (53.85% of isolates; 7/13) was recorded for ofloxacin and enrofloxacin followed by gentamycin (46.15%; 6/13). The results suggest that the diagnosis of APEC with PCR is rapid and more accurate than traditional methods for E. coli identification; moreover, the presence or absence of iss, iutA, and/or fimH genes is not an indicator of in vivo pathogenicity of APEC. Thus, further studies, including a wider range of virulence genes and gene sequencing, are required. In addition, serotyping has no effect on the virulence of APEC.

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Section: Introductionmentioning

confidence: 99%

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

et al. 2020

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“…by E. coli. Pathogenic serotypes of E. coli cause a variety of lesions in immunocompromised hosts as well as in poultry (Kunert et al 2015). E. coli coli, Campylobacter sp., Salmonella sp.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Ščerbová¹,

Lauková²

2016

Polish Journal of Veterinary Sciences

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Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich's microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria. The counts of coliform bacteria varied from 5.69 ± 2.4 log10 CFU/g to 5.73 ± 2.4 CFU/g. Pure colonies were identified using MALDI-TOF MS mass spectrometry and confirmed by phenotypization. Seventy-one strains were allotted to the species E. coli. Sixty-four of those 71 strains caused hemolysis. They were mostly polyresistant to antibiotics. Thirty-two polyresistant strains of E. coli were sensitive to enterocins. These strains were most sensitive to Ent 9296 (26 strains). Moreover, Ent EM41 produced by E. faecium EM41 (isolated from ostrich faeces) inhibited the growth of 20 strains, reaching activity of 100 AU/ml. Our results indicate the possibility of enterocins being used for prevention/reduction of coliforms. Of course, in vivo studies are also being processed.

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“…Escherichia coli patogen pada bangsa unggas menyebabkan infeksi pada sistem pencernakan maupun diluar saluran pencernakan. Kolibasilosis pada unggas menurut Kunert-Filho et al, (2015) menyebabkan septicemia, enteritis, granuloma, omphalitis, sinu sitis, airsacculitis, arthritis/synovitis, peritonitis, pericarditis, perihepatitis, cellulitis, dan swollen head syndrome. Pada burung puyuh kolibasilosis menyebabkan septicemia, pericarditis, perihepatitis, airsacculitis, dan arthritis (Moulin-Schouleur et al, 2007).…”

Section: Pembahasanunclassified

Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro

Khusnan¹,

Prihtiyantoro²,

Kusmanto³

2021

Jurnal Sain Veteriner

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Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds.

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Avian pathogenic Escherichia coli - methods for improved diagnosis

Cited by 8 publications

References 50 publications

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro

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