Avenanthramide Improves Colonic Damage Induced by Food Allergies in Mice through Altering Gut Microbiota and Regulating Hsp70-NF-κB Signaling

Abstract

Food allergies can cause intestinal damage that can exacerbate allergic symptoms, and gut microbiota have been shown to influence allergic development. This study was intended to investigate the effects of Avenanthramide (AVA) on colonic damage induced by food allergy and its mechanism. In Exp. 1, AVA administrations alleviated colonic inflammation in mice challenged with ovalbumin, as shown by decreased concentrations of TNF-α, IL-25 and IL-33. Additionally, the AVA supplementations improved intestinal barrier damage by elevating occludin, ZO-1 and claudin-1 levels. Moreover, AVA inhibited NF-κB phosphorylation and enhanced heat shock protein 70 (Hsp70) expression in the colon. In Exp. 2, apoptozole as a Hsp70 inhibitor was used to explore the Hsp70-NF-κB signaling contribution to AVA function. The AVA additions increased the productions of acetate and butyrate, but decreased propionate. Notably, AVA reduced the colonic abundance of propionate-producing microbes such as Muribaculaceae, but elevated butyrate-producing microbes including Roseburia, Blautia, and Lachnospiraceae_NK4A136_group. Microbial alteration could be responsible for the increased butyrate, and thus the up-regulated Hsp70. However, apoptozole treatment eliminated the effects of AVA. Our study revealed that AVA improved colonic injury and inflammation induced by food allergies, and this mechanism may be mediated by the increased microbial-derived butyrate and involved in the Hsp70-NF-κB signaling.

The alleviated symptoms in ovalbumin‐allergic mice treated with selenium‐enriched tea polysaccharide by modulation of intestinal flora and gut metabolites

“…Therefore, it is worth investigating the changes in typical microorganisms caused by FA in different experimental settings. According to recent studies, Lachnospiraceae_NK4A136_group is a potential probiotic involved in the Hsp70‐NF‐κB signaling to ameliorate colonic damage and inflammation caused by FA (Liu et al., 2023). Alistipes could produce indole, succinic acid, and a small amount of acetic and propionic acids, which increases the expression of tight junction proteins and downregulates the expression of proinflammatory cytokines to enhance the intestinal barrier and reduce diarrhea (Parker et al., 2020).…”

Untargeted metabolomics combined with 16S rRNA sequencing reveals the characteristics of intestinal metabolism and gut microbiota in hazelnut Cor a 14-allergic BALB/c mice

“…Butyrate promoted IL-22 generation by CD4 + T cells through G protein-coupled receptor 41 to relieve intestinal inflammation [46] . Alistipes and Lachnospiraceae_NK4A136_group were expressed at lower levels in both OVA and peanut allergic mice [22,40,45] .…”

“…In addition, Lachnospiraceae_NK4A136_group belonging to the Lachnospiraceae family was commonly thought to be involved in intestinal mucosal barrier protection and immune regulation because of its capacity to produce butyrate [45] . Butyrate promoted IL-22 generation by CD4 + T cells through G protein-coupled receptor 41 to relieve intestinal inflammation [46] .…”

Grape seed proanthocyanidin improves intestinal inflammation in canine through regulating gut microbiota and bile acid compositions

“…Plant polyphenol is a kind of secondary metabolites with phenol structure and can dynamically interact with intestinal bacteria, playing a crucial role in maintaining intestinal health in animals 13 . Polyphenols can directly regulate the composition and diversity of gut microbes, increase the number of beneficial bacteria in the intestine, and thus improve the microbial balance within the intestine 14 . Our previous finding suggested that grape seed proanthocyanidin (GSP), a natural polyphenol compound, changed the intestinal microbial structure and thus activating the intestinal farnesoid X receptor (FXR) signaling pathway to prevent intestinal inflammation in rodent animal models 15 .…”

“…13 Polyphenols can directly regulate the composition and diversity of gut microbes, increase the number of beneficial bacteria in the intestine, and thus improve the microbial balance within the intestine. 14 Our previous finding suggested that grape seed proanthocyanidin (GSP), a natural polyphenol compound, changed the intestinal microbial structure and thus activating the intestinal farnesoid X receptor (FXR) signaling pathway to prevent intestinal inflammation in rodent animal models. 15 However, there are few reports about the application of polyphenols in dogs, and the relationship between GSP and canine gut microbiota requires further exploration.…”