Avaliação histológica dos testículos de cães pré-púberes submetidos à vitrificação com diferentes associações de crioprotetores

Teixeira, Diego Oliveira; Oliveira, Elana da Silva; Fernandes, Juliana; Palomino, Gaby Judith Quispe; Tabosa, Beatriz Evaristo de Alemida; Barbosa, Hélida Tainan Silva; Pinheiro, Breno Queiroz; Silva, Lúcia Daniel Machado da

doi:10.33448/rsd-v10i16.23864

Cited by 2 publications

(3 citation statements)

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“…(6), and vitrification process (7). Warming device (8) and exposure to warming baths (9). Testis fragments after warming processing and analysis (10).…”

Section: Experimental Designmentioning

confidence: 99%

“…The use of the vitrification biotechnique to preserve prepubertal testicular biopsies has been studied in several domestic species, such as pigs [6], mice [7], and dogs [8], to evaluate the viability of protocols. In domestic cats, studies have compared vitrification and slow freezing [9], the use of cryoprotectants for prepubertal cats [10], and the effects of warming temperatures [1,11] on test tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Performance of testis fragment recovery tests (1-4), separation of experimental groups and control, Ovarian Tissue Cryosystem (OTC), Solid Surface Vitrification (SSV) and Straw (STW) (5), exposure of equilibrium solution (ES) and vitrification solution (VS) (6), and vitrification process(7). Warming device(8) and exposure to warming baths(9). Testis fragments after warming processing and analysis(10).…”

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confidence: 99%

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Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Carvalho,

Soares,

Leão

et al. 2023

Animals

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Vitrification is essential for successful tissue cryopreservation and biobanking in wild cats. This study aimed to compare different methods of vitrification (Ovarian Tissue Cryosystem—OTC, Straws—STW, and Solid Surface vitrification—SSV) for testicular fragment vitrification in tom cats. Testicular fragments were recovered from five adult tom cats and subjected to equilibrium vitrification using different cryovials and methods under the same conditions of vitrification solutions and cryoprotectants. The efficiencies of the methods were evaluated using histological analysis of spermatogonia and Sertoli cell nuclei, seminiferous tubular basement membrane detachment, and the gonadal epithelium shrinkage score scale. Cell viability was assessed using Hoechst PI and Terminal deoxynucleotidyl transferase nick end labeling (TUNEL) assay. The results showed that OTC is an effective vitrification method for maintaining the distinction between spermatogonia and Sertoli cells. OTC was similar to the control for basal membrane detachment parameters (p = 0.05). Epithelial shrinkage was low in the SSV group, which showed the highest percentage of viable cells among the vitrified groups (p = 0.0023). The OTC and SSV vitrification methods were statistically similar in terms of the percentage of TUNEL-positive cells (p = 0.05). Therefore, OTC and SSV provide favorable conditions for maintaining viable cat testicular tissue cells after vitrification.

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“…(6), and vitrification process (7). Warming device (8) and exposure to warming baths (9). Testis fragments after warming processing and analysis (10).…”

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Carvalho,

Soares,

Leão

et al. 2023

Animals

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Canine and Feline Testicular Preservation

Silva

2022

Animals

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The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.

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Avaliação histológica dos testículos de cães pré-púberes submetidos à vitrificação com diferentes associações de crioprotetores

Cited by 2 publications

References 14 publications

Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Canine and Feline Testicular Preservation

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