Avaliação De Detergentes E Ultra-Som Para Isolamento De Dna Genômico De Bactérias De Interesse Em Alimentos

Miranda, Robson Rodrigo; Monteiro, Francielli Casanova; Boreiko, Sheila; Bittencourt, Juliana Vitória Messias

doi:10.3895/s1981-36862011000100003s1

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…The 63 B. cereus isolates were stored in nutrient agar under refrigeration, and DNA extraction was carried out according to a technique adapted from Miranda, Monteiro, Boreiko, and Bittencourt (2011). Initially, bacterial cultures were vortexed in liquid medium.…”

Section: Bacterial Dna Isolation and Extractionmentioning

confidence: 99%

Presence of nhe, cytK, and ces in Bacillus cereus isolated from dairy products commercially available in Brazil

Reis

Pereira

Montanhini

et al. 2019

Journal of Food Safety

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The aim of this study was to evaluate the presence of genes (nheABC, cytK, and ces) among bacteria present in dairy products (100 pasteurized milk, 110 UHT milk, and 50 powdered milk). In total, 63 Bacillus cereus isolates were tested and subjected to the detection of genes by using PCR. Regardless of the type of sample analyzed, the most frequent were nheC (82.5%), cytK (68.2%), and nheA (66.6%). The genes nheB and ces were detected in only 23.8% and 3.1% of the isolates, respectively. None of the isolates presented the three genes simultaneously. The combination of nheABC‐cytK was highly prevalent in most of the isolates, present in 25% from pasteurized milk and 20% of those from powdered milk. None of the cultures showed nheABC and ces simultaneously, and the combination of cytK‐ces was found in only one isolate from pasteurized milk (2.7%) and one isolate in powdered milk (6.6%). These results demonstrate the presence of B. cereus toxin genes in dairy products from Brazil. Practical applications Consumption of food contaminated with Bacillus cereus is a risk to public health as this micro‐organism produces diarrheal and emetics toxins. Thus, studies that aim to research genes that code for these toxins can be important tools for dairy industries to establishment of process parameters, limits and actions to control of contamination. This article demonstrated that strains of B. cereus isolated from milk and dairy products may contain genes encoding the toxins (nhe, cytK, and ces), so the risk of food poisoning should not be neglected.

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Section: Bacterial Dna Isolation and Extractionmentioning

confidence: 99%

Presence of nhe, cytK, and ces in Bacillus cereus isolated from dairy products commercially available in Brazil

Reis

Pereira

Montanhini

et al. 2019

Journal of Food Safety

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Comparing protocols of DNA extraction from Escherichia coli: Analysis of purity and concentration by gel electrophoresis

Brito

Santos

Yafawi

et al. 2022

Baghdad J. Biochem. Appl. Biol. Sci.

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Background and objective: Given the difficulty in establishing an ideal method that can successfully extract bacterial deoxyribonucleic acid (DNA) in Gram-negative bacteria, the objective of this work was to compare protocols for the extraction of bacterial DNA and to suggest more practical, faster, and less costly methodologies. Methods: Bacterial species used were Escherichia coli, provided by Dr. Leão Sampaio University Center. The evaluated/adapted methodologies were: sodium dodecyl sulfate (SDS); salting-out; Promega kit and cetyltrimethylammonium bromide (CTAB), and phenol/chloroform. Extracted DNA was examined by the agarose gel electrophoresis. Results: The SDS method revealed the best result in extracting DNA from E. coli. Its advantages include rapidness, low cost, and good concentration of the extracted material. Others methods showed low-quality DNA, probably due to the presence of large amounts of proteins in the cell wall of E. coli, interfering with the quality of the samples. Conclusions: It was concluded that the SDS method is better than others with better DNA quality, low cost, and good efficiency.

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Avaliação De Detergentes E Ultra-Som Para Isolamento De Dna Genômico De Bactérias De Interesse Em Alimentos

Cited by 2 publications

References 7 publications

Presence of nhe, cytK, and ces in Bacillus cereus isolated from dairy products commercially available in Brazil

Presence of nhe, cytK, and ces in Bacillus cereus isolated from dairy products commercially available in Brazil

Comparing protocols of DNA extraction from Escherichia coli: Analysis of purity and concentration by gel electrophoresis

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