Avaliação da morfologia interna de sementes de Terminalia argentea (Combretaceae) pelo teste de raios X

Gomes, Kever Bruno Paradelo; Martins, Rosana de Carvalho Cristo; Martins, Ildeu Soares; Gomes-Júnior, Francisco Guilhien

doi:10.1590/s1806-66902014000400013

Cited by 19 publications

(28 citation statements)

References 14 publications

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“…It also allowed seed morphology to be related to germination or morphology of seedlings of diverse agricultural and forest species (Burg et al, 1994;Battisti et al, 2000;Machado and Cicero, 2003;Oliveira et al, 2003;Mondo and Cicero, 2005;Pupim et al, 2008;Pinto et al, 2009a;Carvalho et al, 2010;Gagliardi and Marcos-Filho, 2011;Kobori et al, 2012;Silva, P.P. et al, 2014;Gomes et al, 2014;Silva, V.N. et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The X-ray test has also been effective in identifying injuries from "moisture" in soybean seeds (Obando-Flor et al, 2004;Forti et al, 2010) and from stink bugs in soybean (Obando-Flor et al, 2004;Pinto et al, 2009b) and in common bean (Forti et al, 2008) seeds, evaluation of fissures in rice seeds (Menezes et al, 2012), and mechanical injuries in maize seeds (Carvalho et al, 1999;Cicero and Banzatto-Junior, 2003;Gomes-Junior and Cicero, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Radiographic analysis to assess the seed structure of Crotalaria juncea L.

Arruda¹,

Cícero²,

Gomes-Júnior³

2016

J. Seed Sci.

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-The X-ray test is a simple, fast, non-destructive, and highly precise test that enables detailed examination of internal seed morphology and identification of seed damage and its location and type. The objective of the present study was to evaluate the efficiency of the X-ray test and the relationship between the structure of Crotalaria juncea seeds and germination. Six lots of Crotalaria juncea seeds of the IAC-KR1 cultivar were subjected to X-ray and germination tests. Normal and abnormal seedlings and dead seeds were photographed and analyzed together with the X-ray images of the seeds. Results showed that analysis of X-ray images is effective in identifying mechanical damage, stink bug damage, and deteriorated tissues in Crotalaria juncea seeds and is able to establish an association between their occurrence and germination.Index terms: image analysis, germination, internal morphology.Análise radiográfica para avaliação da estrutura de sementes de Crotalaria juncea L.RESUMO -O teste de raios X é um teste rápido, simples, não destrutivo e de alta precisão, que possibilita examinar com detalhes, a morfologia interna da semente, identificar áreas danificadas, sua localização e tipos de danos. O objetivo na presente pesquisa foi avaliar a eficiência do teste de raios X e relacionar a estrutura de sementes de Crotalaria juncea com a germinação. Foram utilizados seis lotes de sementes de Crotalaria juncea, cultivar IAC-KR1. As sementes foram submetidas aos testes de raios X e germinação. As plântulas normais, anormais e sementes mortas foram fotografadas e analisadas paralelamente às imagens radiográficas das sementes. Verificou-se que análise de imagens radiográficas possibilita a identificação de danos mecânicos, danos por percevejos e tecidos deteriorados em sementes de Crotalaria juncea, com efeitos negativos à germinação.Termos para indexação: análise de imagens, germinação, morfologia interna.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radiographic analysis to assess the seed structure of Crotalaria juncea L.

Arruda¹,

Cícero²,

Gomes-Júnior³

2016

J. Seed Sci.

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“…It has been shown that X-ray imaging allows the improvement of the physical and physiological quality of seed lots by removing internally damaged, poorly formed, or empty seeds, as was observed for Cecropia pachystachya Trec. seeds (Pupim et al, 2008), seeds of arboreal Lauraceae species (Carvalho et al, 2009), Tabebuia heptaphylla (Amaral et al, 2011), and Terminalia argentea (Gomes et al, 2014). It also allows for the identification of damaged or abnormal embryos and their removal from work samples, as observed in Lithraea molleoides (Machado & Cicero, 2003) and Malpighia emarginata D.C. (Nassif & Cicero, 2006).…”

Section: Eamentioning

confidence: 99%

“…The native forest species undergo high rates of predation, insect attack, empty fruit, and deficiency in the formation of the embryo, all of which compromise the performance of the seed and the establishment of seedlings in the field (Gomes, Martins, Martins, & Junior, 2014). Studies on the quality of the native forest seeds represent only 0.1% of the species found in the Rules for Seed Testing (Brasil, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…(Pupim, Novembre, Carvalho, & Cicero, 2008), Cedrela fissilis (Masetto, Faria, & Queiroz, 2008), Tabebuia heptaphylla (Amaral, Martins, Forti, Cicero, & Marcos Filho, 2011), Xylopia aromatica (Socolowski, Cicero, & Vieira, 2011), and Syagrus romanzoffiana (Cham) Glassman (Sturião, Landgraf, & Rosa, 2012), and in the morphological characterization of embryos of Tecoma stans L. JUSS. ex KUNTH (Socolowski & Cicero, 2008), the measurement of internal free areas in seeds of Acca sellowiana (Silva, Sarmento, Silveira, Silva, & Cicero, 2013), and the viewing of the internal morphology of seeds of Terminalia argentea (Gomes et al, 2014 Given the scarcity of information on the internal morphology and the effects of desiccation on achenes of species in the genus Anacardium, the objective of this study was to verify the efficiency of X-ray testing in evaluating the morphology and viability of A. othonianum achenes subjected to desiccation.…”

Section: Introductionmentioning

confidence: 99%

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<b>Radiographic image analysis of <i>Anacardium othonianum</i> Rizz (anacardiaceae) achenes subjected to desiccation

et al. 2017

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ABSTRACT. Studies evaluating the internal morphology and seed quality of native species are essential for successful conservation programs. Our aim was to verify the efficiency of X-ray imagery in evaluating cashew-tree-of-the-cerrado (Anacardium othonianum Rizz.) achene viability after desiccation. The achenes were collected at 12% water content (w.b.) and dried in silica gel until they reached 10, 8, 6, and 4% (w.b.). The fruit morphology and the quality of the seeds were evaluated by X-ray test together with vigor, electrical conductivity and emergence tests. Achenes with different water contents were exposed to an Xray machine at 18 kV for 11 s and were thereafter submitted to emergence tests. The images were analyzed, and the achenes were classified based on internal morphology as completely full, malformed, or empty. These results were compared to those from the emergence tests. The statistical design was a complete randomized factorial (5 x 3). Desiccation to 4% (w.b.) did not damage or modify the internal structures. Xray was efficient in evaluating the internal morphology and detecting achene quality, making it possible to remove empty and abnormal fruit and form vigorous seed lots, reducing the cost of storage and bedding plant production for this native species.Keywords: X-Ray, fruit tree, cerrado, desiccation, internal morphology.Análise de imagens radiográficas em aquênios de Anacardium othonianum Rizz (anacardiaceae) submetidos à secagem RESUMO. Estudos referentes à avaliação da morfologia interna e da qualidade de sementes de espécies nativas são de fundamental importância para o sucesso dos programas de conservação. Objetivou-se com este estudo verificar a eficiência do teste com raios X na avaliação da viabilidade de aquênios de caju-deárvore-do-cerrado (Anacardium othonianum Rizz.) submetidos à secagem. Para tal, aquênios de A. othonianum Rizz foram coletados com 12% (b.u.) e submetidos à secagem em sílica gel até a obtenção dos teores de água de 10, 8, 6 e 4% (b.u.). Os frutos foram avaliados quanto à morfologia interna e qualidade física pelo teste de raios X, e o vigor das sementes pelos testes de germinação, condutividade elétrica e emergência de plântulas. Para a obtenção das imagens radiográficas, aquênios com diferentes teores de água foram expostos à radiação em um aparelho de raios X digital "Faxitron HP", MX-20 à intensidade de 18 kV por 11 segundos, e posteriormente foram submetidos ao teste de emergência. As imagens radiográficas foram analisadas e os aquênios classificados de acordo com a morfologia interna em três categorias: Cheios, mal formados e vazios. O delineamento estatístico utilizado foi o inteiramente casualizado em esquema fatorial (5 x 3). A secagem dos frutos até 4% (b.u.) não danificou ou alterou as estruturas internas dos aquênios. O teste de raios X é eficiente na avaliação da morfologia interna e na detecção da qualidade de aquênios de A. othonianum Rizz, possibilitando a separação de aquênios vazios ou com anormalidades embrionárias e permite a formação de lotes ...

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In vitro propagation of Campomanesia rufa: An endangered fruit species

et al. 2018

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The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3 was also tested on in vitro shoot elongation. For oxidation control, the medium was supplemented with PVP (polyvinylpyrrolidone) and for callus induction, 2.4-D (dichlorophenoxyacetic acid). The results showed 68% germination regardless of GA3 concentration. In the propagation stage, BAP at 4.5 µM induced a higher number of shoots (4.53) and LED lamps as the light source combined with the culture medium with 1 μM BAP induced a higher number of shoots (4.08). The highest average of C. rufa length (31.9 mm) was obtained using 8.8 µM GA3. The use of PVP (584.3 uM) controls up to 27.3% oxidation in young leaf explants. The use of 10 µM 2.4-D leads to a higher callus formation (58.7 %). Therefore, it can be concluded that the use of BAP is efficient in the induction of shoots, PVP controls oxidation leaf segments, and 2.4-D induces callus in C. rufa.

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Avaliação da morfologia interna de sementes de Terminalia argentea (Combretaceae) pelo teste de raios X

Cited by 19 publications

References 14 publications

Radiographic analysis to assess the seed structure of Crotalaria juncea L.

Radiographic analysis to assess the seed structure of Crotalaria juncea L.

<b>Radiographic image analysis of <i>Anacardium othonianum</i> Rizz (anacardiaceae) achenes subjected to desiccation

In vitro propagation of Campomanesia rufa: An endangered fruit species

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