Avaliação da atividade cicatrizante do jucá (Caesalpinia ferrea Mart. ex Tul. var. ferrea) em lesões cutâneas de caprinos

Abstract

RESUMO:O objetivo deste estudo foi demonstrar os efeitos do tratamento tópico do jucá (Caesalpinia ferrea) em feridas cutâneas. Quinze caprinos machos sem raça definida foram divididos em 3 grupos de acordo com o pós-cirúrgico (7 o , 14 o e 21 o dias). As feridas experimentais foram tratadas com a pomada composta pela casca da Caesalpinia ferrea em pó misturada com a vaselina estéril e as do grupo controle apenas com a vaselina esterilizada. A aplicação diária da pomada e da vaselina estéril foi realizada sobre ferida circular padronizada de 16 cm 2 de área na região torácica de cada animal. As avaliações das feridas foram feitas do ponto de vista clínico, bacteriológico, morfométrico e histopatológico nos períodos pré-determinados (7 o , 14 o e 21 o dias). Morfometricamente, as feridas do controle apresentaram áreas cirúrgicas menores e grau de contração maior que as do grupo tratado, entretanto, histologicamente, houve completa epitelização das feridas tratadas no 21 o dia, enquanto que as feridas do grupo controle necessitavam de mais tempo para resolução do processo cicatricial. No exame microbiológico realizado no momento da produção da ferida, não se observou crescimento bacteriano e no momento das biópsias, identificou-se a presença de bactérias da família Enterobacteriaceae e Staphylococcus aureus, sendo que a partir do 14 o dia observou-se Staphylococcus aureus apenas no grupo controle. A utilização tópica da pomada de Caesalpinia ferrea apresentou eficiência significativa no auxílio da reparação cicatricial de feridas cutâneas de caprinos.Palavras-chave: ferida, cicatrização, caprinos, Caesalpinia ferrea ABSTRACT: Evaluation of the Brazilian ironwood (Caesalpinia ferrea Mart. ex Tul. var. ferrea) healing activity on cutaneous lesions of goats. The aim of this study was to demonstrate the effects of the topical treatment with Brazilian ironwood (Caesalpinia ferrea) on cutaneous wounds. Fifteen male mongrel goats were divided into 3 groups according to the postoperative period ((7 th , 14 th and 21 st days). The experimental wounds were treated with an ointment composed of Brazilian wood powder bark mixed with sterile vaseline, whereas controls were only treated with sterile Vaseline. The ointment and the sterile vaselin were daily applied on a standardized circular wound (16 cm 2 area) in the thoracic region of each animal. Clinical, bacteriological, morphometric and histopathological evaluations were performed in the wounds at predetermined periods (7 th , 14 th and 21 st postoperative days). Morphometrically, control wounds had smaller surgical areas and greater degree of contraction than those from the treated group. However, histologically, there was a complete epithelialization of the treated wounds on the 21 st day, whereas control wounds required longer time for healing. In the microbiological evaluation performed at the moment of wound production, there was no bacterial growth. During biopsies, Enterobacteriaceae bacteria and Staphylococcus aureus were identified; from the 14 th day, the l...

Atividade antibacteriana e cicatrizante do óleo de buriti Mauritia flexuosa L.

“…O percentual de contração é um parâmetro morfométrico frequentemente utilizado como indicador de resolução do processo cicatricial (BATISTA et al, 2010). Esse fenômeno ocorre devido à diferenciação dos fibroblastos em miofibroblastos que apresentam capacidade contrátil e promovem força de tensão e união dos bordos da ferida (OLIVEIRA et al, 2010).…”

Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests

“…Previous studies have shown biological activities of the extracts of fruit and stem L. ferrea, such as antiinflammatory (Carvalho et al 1996;Pereira et al 2012), and antimicrobial, whereas fruit extract can inhibit in vitro growth of oral pathogens (Sampaio et al 2009), and antioxidant (Silva et al 2011), but this was the first screening with different extracts obtained from L. ferrea evaluated for Leishmania species. However, previous studies showed that the topical use of L. ferrea ointment was significantly efficient to help healing cutaneous wounds in goats (Oliveira et al 2010). Studies that seek new therapeutic forms against cutaneous leishmaniasis evaluate several parameters to determine the efficacy of the compound and its use (Schwartz et al 2014).…”

“…Libidibia ferrea has a wide distribution, and many economic and pharmacological properties, being used from wood production to the afforestation of streets and avenues (Souza and Lorenzi 2012). Pharmacological studies of extracts obtained from L. ferrea have demonstrated its biological anti-inflammatory (Carvalho et al 1996), bactericidal (Sampaio et al 2009), hypoglycemic and antioxidant potentials (Batubara et al 2009) and healing activity (Oliveira et al 2010). Chemical studies with this species have shown the presence of phenolic compounds with possible pharmacological applications, such as chalcones and polyphenols (Nakamura et al 2002;Nozaki et al 2007).…”

Comparing open wound measuring methods popularly used in experimental studies

“…Healing assays using experimental animals, particularly those assessing wound healing promoter substances, are conducted worldwide (LOPES et al, 2005;OLIVEIRA et al, 2010;NAYAK et al, 2011). Wound contraction is frequently assessed in these experiments because it allows for a better understanding of the healing process evolution and the maturation of the scar, which represent myofibroblast activity, and the buildup and maturation of collagen fibers (COELHO et al, 1999).…”

“…The animals were divided into three groups regarding their assessment dates (four, seven and 14 days). Half of the wounds in each group were treated with saline solution and the other half with petroleum jelly (Vaseline), typically used as mock treatments to control wound healing assays (LOPES et al, 2005;OLIVEIRA et al, 2010;NAYAK et al, 2011). After the experimental period, the animals were euthanized according to resolution nº 1000 may 12 th 2012 (CFMV).…”