Avaliação da acurácia de testes laboratoriais para detecção de amostras de Klebsiella pneumoniae produtora de betalactamase de espectro estendido

Pereira, Andrea dos Santos; Filho, José Rodrigues do Carmo; Tognim, Maria Cristina Bronharo; Sader, Hélio S.

doi:10.1590/s1676-24442003000400007

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…In Rio Grande do Sul, Freitas et al (6) and d’Azevedo et al (1) also observed that these two species were the most prevalent among ESBL producing microorganisms, confirming international multicenter studies (7, 8, 12, 13, 17). These results are very important as K. pneumoniae is the most frequent gram-negative bacteria involved in hospital-acquired infections and nosocomial outbreaks (5, 6, 11). …”

Section: Discussionmentioning

confidence: 99%

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates

Oliveira¹,

Salla²,

Lara³

et al. 2010

Braz. J. Microbiol.

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The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.

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Section: Discussionmentioning

confidence: 99%

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates

Oliveira¹,

Salla²,

Lara³

et al. 2010

Braz. J. Microbiol.

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“…Dentre as enterobactérias produtoras de ESBL, Enterobacter sp foi responsável por 30,3%. É sabido que a diminuição na permeabilidade da bactéria aos antimicrobianos e a produção de outras β-lactamases induzíveis podem mascarar a presença de ESBL 19,20 . Airpin e cols 21 relataram que 49% dos isolados produtores de ESBL eram E. aerogenes, seguidos de 21% de K. pneumoniae e 15% de E. coli.…”

Section: Figura 1 -Perfil De Resistência Dos Isolados Produtores De Eunclassified

Enterobactérias produtoras de ESBL em Passo Fundo, estado do Rio Grande do Sul, Brasil

Lago

Fuentefria

2010

Rev. Soc. Bras. Med. Trop.

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RESUMOIntrodução: O principal mecanismo de resistência emergente entre Enterobacteriaceae é a produção de β-lactamases de espectro estendido, enzimas capazes de hidrolisar cefalosporinasde-amplo-espectro, que são bastante utilizadas na terapia antimicrobiana de infecções por enterobactérias. Embora a resistência a esses agentes apresente grande variabilidade geográfica, os índices de resistência são elevados em diversos países. ABSTRACT Introduction:The main emerging resistance mechanism relating to Enterobacteriaceae is represented by production of extended spectrum β-lactamases (ESBLs). These enzymes have the capacity to hydrolyze broad-spectrum cephalosporins and are greatly used for antimicrobial chemotherapy on enterobacterial infections. Although resistance to these agents presents remarkable geographical variability, the resistance rates are high in many countries. Methods: A retrospective observational cross-sectional descriptive study was developed to evaluate the frequency of ESBLs among Enterobacteriaceae strains obtained from Hospital São Vicente de Paulo, Brazil. Results: ESBL production was noted in 24.8% (n = 208/838) of the isolates evaluated. Isolates of Escherichia coli represented 46.2% (n = 96/208) of the ESBL producers, followed by Enterobacter species (30.3%; n = 63/208). The sensitivity of these isolates to meropenem was 91.4% and to piperacillin/tazobactam, 67.4%. Conclusions: The ESBL levels founded confirm the worldwide concern regarding this resistance mechanism.

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“…Esta família de bactérias é de grande importância no ambiente hospitalar, não apenas por seus fatores de virulência, mas porque podem desenvolver resistência a várias classes de antimicrobianos. Finalmente, foram incluídas 10 cepas de bactérias ESBL, as quais representam um dos mais importantes problemas de resistência bacteriana nos hospitais brasileiros (Pereira et al, 2005). São bactérias capazes de produzir beta-lactamases que inativam os antibióticos betalactâmicos.…”

Section: Resultsunclassified

Triazenos e atividade antibacteriana

Hörner¹,

Giglio²,

Santos³

et al. 2008

Rev. Bras. Cienc. Farm.

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Avaliação da acurácia de testes laboratoriais para detecção de amostras de Klebsiella pneumoniae produtora de betalactamase de espectro estendido

Cited by 9 publications

References 22 publications

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates

Enterobactérias produtoras de ESBL em Passo Fundo, estado do Rio Grande do Sul, Brasil

Triazenos e atividade antibacteriana

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