Auxin signaling: a big question to be addressed by small molecules

Ma, Qian; Grones, Peter; Robert, Stéphanie

doi:10.1093/jxb/erx375

Cited by 76 publications

(56 citation statements)

References 194 publications

(283 reference statements)

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“…It has long been known that the auxin-binding pocket of SCF TIR1/AFB is promiscuous, a feature which was heavily investigated during the early years of auxin research in the 1940s (57)(58). During this time, several auxinic compounds were discovered including NAA, 2,4-D and picolinate auxins such as picloram (59), which are widely used today for basic research and agricultural applications. The 2,4-D and NAA modes of action are similar to that of IAA, as they also enhance the binding affinity between TIR1 and the AUX/IAAs.…”

Section: Discussionmentioning

confidence: 99%

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development

Vain

Raggi

Ferro

et al. 2018

Preprint

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Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1neddylation is required for SCF TIR1/AFB functionality as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin1 to 4 (RN1 to 4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical and morphological levels. This selective activity is explained by their ability to promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, via a genetic screen using RN4, we revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions.

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Section: Discussionmentioning

confidence: 99%

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development

Vain

Raggi

Ferro

et al. 2018

Preprint

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“…[7−12] Auxin is involved in an array of diverse responses during the life cycle of a plant, such as cell elongation, cell division, root and shoot growth, apical dominance, flower and fruit development, gravi-and phototropism and many more. [13] While auxin at low levels is actively regulating the above processes, it is inhibitory at higher concentrations. Therefore, it is crucial for proper plant development to control the levels of auxin in a given tissue, e.g.…”

Section: Auxin: Crucial Plant Hormonementioning

confidence: 99%

Auxin Amidohydrolases – From Structure to Function: Revisited

Smolko¹,

Ludwig‐Müller²,

Salopek‐Sondi³

2018

Croat. Chem. Acta

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The control of plant growth and development is a well-coordinated process between exogenous and endogenous signals. Auxins are plant hormones belonging to the endogenous signals, which control a vast array of different processes. While auxins are growth promoting at low concentrations, higher levels are often inhibitory. Therefore, the tight control of auxin concentrations in a given plant tissue is essential. Among several processes that participate in auxin homeostasis, we focused herein on the process of reversible auxin conjugation that considers the synthesis of inactive auxin conjugates, which can be hydrolyzed back to the active form by so called auxin conjugate hydrolases. Although these proteins have been known for quite some time, their role in plants is still not clear, especially since novel hydrolases with different substrate specificities have been isolated. Thus, we have revisited the knowledge about auxin hydrolases, from their structure and biochemistry to the role in plant development and in dealing with unfavorable climate conditions.

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“…Auxins represent a category of low molecular weight organic acids containing both an aromatic ring and a carboxylic acid side chain within 0.55 Å distance so that the compounds can be bioactive [1]. Indole-3-acetic acid (IAA), 4-chloroindole-3-acetic acid (4-Cl-IAA), and phenylacetic acid (PAA) are three naturally occurring compounds with direct auxin activity in plants [1,2]. Indole-3-butyric acid (IBA), although presenting similar structure and function in plant growth and development, is defined here as an IAA precursor because it can directly evoke auxin signaling and response only after being converted to IAA [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Indole-3-butyric acid (IBA), although presenting similar structure and function in plant growth and development, is defined here as an IAA precursor because it can directly evoke auxin signaling and response only after being converted to IAA [3][4][5]. In addition to this, a diverse group of synthetic compounds with similar structure and activity of the endogenous auxins (termed synthetic auxin analogues), such as 1-naphthaleneacetic acid (NAA), 2-(2,4-dichlorophenoxy) propionic acid (2,4-DP), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, picloram, quinclorac, and so on, is widely used as chemical tools in scientific and agronomic practices [2]. Amongst all the discovered naturally occurring endogenous auxins in plants, IAA has been well and widely characterized so far, and auxin refers to IAA in plants by strict definition [2].…”

Section: Introductionmentioning

confidence: 99%

The Roles of Auxin Biosynthesis YUCCA Gene Family in Plants

Cao

Yang

Shang

et al. 2019

IJMS

142

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Auxin plays essential roles in plant normal growth and development. The auxin signaling pathway relies on the auxin gradient within tissues and cells, which is facilitated by both local auxin biosynthesis and polar auxin transport (PAT). The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA)/YUCCA (YUC) pathway is the most important and well-characterized pathway that plants deploy to produce auxin. YUCs function as flavin-containing monooxygenases (FMO) catalyzing the rate-limiting irreversible oxidative decarboxylation of indole-3-pyruvate acid (IPyA) to form indole-3-acetic acid (IAA). The spatiotemporal dynamic expression of different YUC gene members finely tunes the local auxin biosynthesis in plants, which contributes to plant development as well as environmental responses. In this review, the recent advances in the identification, evolution, molecular structures, and functions in plant development and stress response regarding the YUC gene family are addressed.

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Auxin signaling: a big question to be addressed by small molecules

Abstract: This review summarizes the current knowledge of auxin signaling and emphasizes how chemical genomics could help to unravel the complexity of the TIR1/AFB–Aux/IAA co-receptor system.

Cited by 76 publications

References 194 publications

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development

Auxin Amidohydrolases – From Structure to Function: Revisited

The Roles of Auxin Biosynthesis YUCCA Gene Family in Plants

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