Auxin promotion of seedling growth via ARF5 is dependent on the brassinosteroid‐regulated transcription factors BES1 and BEH4

Galstyan, Anahit; Nemhauser, Jennifer L.

doi:10.1002/pld3.166

Cited by 18 publications

(7 citation statements)

References 64 publications

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“…Previous reports suggested that interactions between TFs involved in auxin and BR signaling might act as the points of auxin-BR crosstalk; one case is the response of SAUR15 to BR and auxin. The expression of SAUR15 depends on the combined binding of BES1 and MONOPTEROS/ARF5 within its promoter harboring a hormone up at dawn-type E-box and AuxRE cis elements, respectively (Walcher and Nemhauser, 2012). In our study, we also found that there were auxin response elements within promoter of PdC3H17 (data not shown), which inferred that PdC3H17 could combine these two hormones through BES1 and other ARFs during xylem formation.…”

Section: Discussionsupporting

confidence: 49%

Brassinosteroid Signaling Converges With Auxin-Mediated C3H17 to Regulate Xylem Formation in Populus

et al. 2020

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Brassinosteroid (BR) signaling has long been reported to have an effect on xylem development, but the detailed mechanism remains unclear, especially in tree species. In this study, we find PdC3H17, which was demonstrated to mediate xylem formation driven by auxin in our previous report, is also involved in BR-promoted xylem development. Y1H analysis, EMSA, and transcription activation assay confirmed that PdC3H17 was directly targeted by PdBES1, which is a key transcriptional regulator in BR signaling. Tissue specificity expression analysis and in situ assay revealed that PdC3H17 had an overlapping expression profile with PdBES1. Hormone treatment examinations verified that xylem phenotypes in PdC3H17 transgenic plants, which were readily apparent in normal condition, were attenuated by treatment with either brassinolide or the BR biosynthesis inhibitor propiconazole. The subsequent quantitative real-time polymerase chain reaction (qRT-PCR) analyses further revealed that BR converged with PdC3H17 to influence transcription of downstream xylem-related genes. Additionally, the enhancement of xylem differentiation by auxin in PdC3H17 overexpression plants was significantly attenuated compared with wild-type and dominant negative plants due to BR deficiency, which suggested that the BRand auxin-responsive gene PdC3H17 acted as an mediation of these two hormones to facilitate xylem development. Taken together, our results demonstrate that BR signaling converges with auxin-mediated PdC3H17 to regulate xylem formation in Populus and thus provide insight into the regulation mechanism of BRs and the crosstalk with auxin signaling on xylem formation.

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Section: Discussionsupporting

confidence: 49%

Brassinosteroid Signaling Converges With Auxin-Mediated C3H17 to Regulate Xylem Formation in Populus

et al. 2020

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“…For instance, the DELLA protein GAIPB was up-regulated in the gibberellin signaling pathway, and CYCD3 was down-regulated in the brassinosteroid signaling pathway. ARF5 and the BES1 co-regulated a subset of growth-promoting genes, and BES1 play a major role in auxin response in young seedlings [61]. During the cherry immunity process, BES1 and BEH2 could be responsive to auxin signal transduction in fruit rather than BRs.…”

Section: Discussionmentioning

confidence: 98%

Transcriptome Integrated with Metabolome Reveals the Molecular Mechanism of Phytoplasma Cherry Phyllody Disease on Stiff Fruit in Chinese Cherry (Cerasus pseudocerasus L.)

Chen

WeiXing

et al. 2021

Agriculture

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Phytoplasma-infected Chinese cherry (Cerasus pseudocerasus L.) exhibits symptoms of phyllody and stiff fruit. To reveal the molecular mechanism of stiff fruit, the current study integrated transcriptome with metabolome. Results showed that the differentially expressed genes and the differentially accumulated metabolites were related to a high proportion of two aspects: pathogen resistance and signaling or regulatory functions, and the molecular mechanism of stiff fruit that were majorly induced by plant biotic stress response via phytohormones signal transduction, especially signal pathways of salicylic acid, auxin, and abscisic acid. Notably, there was a large overlap between phytoplasma stress response and drought stress response genes. Phytohormone content displayed significant difference that abscisic acid and salicylic acid content of phytoplasma-infected fruit were higher than that of healthy fruit, whereas zeatin, jasmonic acid, and IAA showed the opposite results. In addition, the expression of key candidate genes, including IAA4, IAA9, IAA14, IAA31, ARF5, ARF9, GH3.1, GH3.17, SAUR20, SAUR32, SAUR40, PR1a, PRB1, TGA10, SnRK2.3, and AHK2, was responsible for cherry stiff fruit. In conclusion, the current study contributed a foundation for understanding the molecular mechanism of cherry phyllody disease on stiff fruit, a better understanding of fruit development, and found the potential candidate genes involved in cherry stiff fruit, which could be used for further research in associated fields.

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“…BES1 regulates the vascular cell development in the root apex and root growth mediated by the type one protein phosphatases [ 37 , 38 ]. Besides, BES1 acts to promote seedling growth in the auxin pathway [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Maize ZmBES1/BZR1-3 and -9 Transcription Factors Negatively Regulate Drought Tolerance in Transgenic Arabidopsis

Feng

Liu

Cao

et al. 2022

IJMS

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The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1(BZR1) transcription factors play crucial roles in plant growth, development, and stress response. However, little is known about the function of maize’s BES1/BZR1s. In this study, the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes were cloned from maize’s inbred line, B73, and they were functionally evaluated by analyzing their expression pattern, subcellular localization, transcriptional activation activity, as well as their heterologous expression in Arabidopsis, respectively. The results of the qRT-PCR showed that the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes were predominantly expressed in the root, and their expression was significantly down-regulated by drought stress. The ZmBES1/BZR1-3 and ZmBES1/BZR1-9 proteins localized in the nucleus but showed no transcriptional activation activity as a monomer. Subsequently, it was found that the heterologous expression of the ZmBES1/BZR1-3 and ZmBES1/BZR1-9 genes in Arabidopsis decreased drought tolerance, respectively. The transgenic lines showed a more serious wilting phenotype, shorter root length, lower fresh weight, and higher relative electrolyte leakage (REL) and malondialdehyde (MDA) content compared to the control under drought stress. The RNA-sequencing data showed that the 70.67% and 93.27% differentially expressed genes (DEGs) were significantly down-regulated in ZmBES1/BZR1-3 and ZmBES1/BZR1-9 transgenic Arabidopsis, respectively. The DEGs of ZmBES1/BZR1-3 gene’s expressing lines were mainly associated with oxidative stress response and amino acid metabolic process and enriched in phenylpropanoid biosynthesis and protein processing in the endoplasmic reticulum. But the DEGs of the ZmBES1/BZR1-9 gene’s expressing lines were predominantly annotated with water deprivation, extracellular stimuli, and jasmonic acid and enriched in phenylpropanoid biosynthesis and plant hormone signal transduction. Moreover, ZmBES1/BZR1-9 increased stomatal aperture in transgenic Arabidopsis under drought stress. This study indicates that ZmBES1/BZR1-3 and ZmBES1/BZR1-9 negatively regulate drought tolerance via different pathways in transgenic Arabidopsis, and it provides insights into the underlying the function of BES1/BZR1s in crops.

show abstract

Auxin promotion of seedling growth via ARF5 is dependent on the brassinosteroid‐regulated transcription factors BES1 and BEH4

Cited by 18 publications

References 64 publications

Brassinosteroid Signaling Converges With Auxin-Mediated C3H17 to Regulate Xylem Formation in Populus

Brassinosteroid Signaling Converges With Auxin-Mediated C3H17 to Regulate Xylem Formation in Populus

Transcriptome Integrated with Metabolome Reveals the Molecular Mechanism of Phytoplasma Cherry Phyllody Disease on Stiff Fruit in Chinese Cherry (Cerasus pseudocerasus L.)

Maize ZmBES1/BZR1-3 and -9 Transcription Factors Negatively Regulate Drought Tolerance in Transgenic Arabidopsis

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