Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development

Yao, Xiaozhen; Tian, Lei; Yang, Jun; Zhao, Yong; Zhu, Ying-Xiu; Dai, Xinhua; Zhao, Yunde; Yang, Zhong‐Nan

doi:10.1371/journal.pgen.1007397

Cited by 67 publications

(54 citation statements)

References 73 publications

(84 reference statements)

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“…4A). This is consistent with published data on localized auxin synthesis by tapetum cells prior to pollen maturation 33,34 . Furthermore, we detected strong auxin signaling in approximately four cells at the very tips of the anthers.…”

Section: Live Imaging Of Flower Developmentsupporting

confidence: 93%

Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy

Valuchova

Mikulková

Pecinkova

et al. 2019

Preprint

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In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.

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Section: Live Imaging Of Flower Developmentsupporting

confidence: 93%

Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy

Valuchova

Mikulková

Pecinkova

et al. 2019

Preprint

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“…Auxin biosynthesis through YUC2 and YUC6 is also important for anther dehiscence (Cheng et al, 2006). As shown in Figure 3 in Yao et al (2018), the yuc2 yuc6 mutant exhibits defective anther dehiscence. In this paper, we further demonstrated the importance of auxin signaling transduction mediated by ARF17 in endothecium lignification, which was completely blocked in arf17 (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…E, Epidermis; En, endothecium. formation (Cheng et al, 2006;Yao et al, 2018). Although ARF17 is a member of the auxin-responsive factor family, it is unclear if it is responsive to auxin.…”

Section: Delayed Lignification In the Endothecium Leads To Defective mentioning

confidence: 99%

AUXIN RESPONSE FACTOR17 Directly Regulates MYB108 for Anther Dehiscence

Wang

Feng

et al. 2019

Plant Physiol.

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The timely release of mature pollen following anther dehiscence is essential for reproduction in flowering plants. AUXIN RESPONSE FACTOR17 (ARF17) plays a crucial role in pollen wall pattern formation, tapetum development, and auxin signal transduction in anthers. Here, we showed that ARF17 is also involved in anther dehiscence. The Arabidopsis (Arabidopsis thaliana) arf17 mutant exhibits defective endothecium lignification, which leads to defects in anther dehiscence. The expression of MYB108, which encodes a transcription factor important for anther dehiscence, was dramatically downregulated in the flower buds of arf17. Chromatin immunoprecipitation assays and electrophoretic mobility shift assays showed ARF17 directly binds to the MYB108 promoter. In an ARF17-GFP transgenic line, in which ARF17-GFP fully complements the arf17 phenotype, ARF17-GFP was observed in the endothecia at anther stage 11. The GUS signal driven by the MYB108 promoter was also detected in endothecia at late anther stages in transgenic plants expressing promoterMYB108::GUS. Thus, the expression pattern of both ARF17 and MYB108 is consistent with the function of these genes in anther dehiscence. Furthermore, the expression of MYB108 driven by the ARF17 promoter successfully restored the defects in anther dehiscence of arf17. These results demonstrated that ARF17 regulates the expression of MYB108 for anther dehiscence. Together with its function in microcytes and tapeta, ARF17 likely coordinates the development of different sporophytic cell layers in anthers. The ARF17-MYB108 pathway involved in regulating anther dehiscence is also discussed.

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“…4A, Supplemental Table 9). This reflects the requirement of YUCCA6 and auxin production in corresponding cells for viable Arabidopsis pollen development (Yao et al, 2018). Cowpea homologs of the Arabidopsis ARF19 , AIR12 and DRB1 auxin-responsive genes were also upregulated during male gametophyte formation (Fig 4B).…”

Section: Resultsmentioning

confidence: 71%

“…Auxin biosynthesis and auxin-responsive genes were upregulated during early cowpea pollen development, reflecting the functional requirement for auxin in early Arabidopsis pollen development (Yao et al, 2018). Auxin biosynthesis is also considered important during Arabidopsis female gametophyte development.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

Gursanscky

Mazurkiewicz

Juranić

et al. 2020

Preprint

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Molecular knowledge of pathways regulating seed formation in legumes, remains scarce. Thirteen isolated cell-type transcriptomes were developed, spanning temporal events of male and female gametogenesis and seed initiation, to examine pathways involved in cowpea seed formation. In situ hybridization confirmed localization of in silico identified cell-specific genes, verifying transcriptome utility. Cowpea and Arabidopsis reproductive cells showed some conservation in regulators enabling cell-type expression as some cowpea cell-specific genes promoters and their Arabidopsis homologs directed expression to identical reproductive cell-types in transgenic plants. In silico analyses revealed gene expression similarities and differences with genes in pathways regulating reproductive events in other plants. Meiosis-related genes were expressed at mitotic stages of gametogenesis and during sporophytic development in cowpea. Plant hormone pathways showing preferential expression at particular reproductive stages were identified. Expression of epigenetic pathways, resembling those found in Arabidopsis, including microRNA mediated gene silencing, RNA directed DNA methylation and histone modification were associated with particular stages of male and female gametophyte development, suggesting roles in gametogenic cell specification and elaboration. Analyses of cell-cycle related gene expression in mature cowpea female gametophytes, indicated that the egg and central cell were arrested at the G1/S and G2/M cell cycle phases, respectively, prior to fertilization. Pre-fertilization female gametophyte arrest was characterized by barely detectable auxin biosynthesis gene expression levels, and elevated expression of genes involved in RNA-mediated gene silencing and histone modification. These transcriptomes provide a useful resource for additional interrogation to support functional analyses for development of higher yielding cowpea and syntenic legume crops. One sentence summary Transcriptomes from cowpea reproductive cell-types isolated in temporal sequence from meiosis to seed initiation revealed cell-type gene expression profiles enabling comparison with gene expression pathways known to function in cell specification and reproductive development in other plants.

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Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development

Cited by 67 publications

References 73 publications

Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy

Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy

AUXIN RESPONSE FACTOR17 Directly Regulates MYB108 for Anther Dehiscence

Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

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