Auxin Fuels the Cell Cycle Engine During Lateral Root Initiation

Vanneste, Steffen; Inzé, Dirk; Beeckman, Tom

doi:10.1002/9780470988923.ch8

Cited by 3 publications

(6 citation statements)

References 94 publications

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“…It has been reported that most of the cell types leave the meristem in G1-phase, and lately G1-to-S transition is only triggered in perycicle cells to initiate lateral root ( Vanneste et al, 2007 ). In Arabidopsis pericycle, cells adjacent to protoxilem poles have been proposed to go on the cell cycle without interruption when they pass through transition, elongation and differentiation zones ( Dubrovsky et al, 2008 ).…”

Section: Resultsmentioning

confidence: 99%

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Transition zone cells reach G2 phase before initiating elongation in maize root apex

Alarcón

Salguero

2017

Biology Open

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Root elongation requires cell divisions in the meristematic zone and cell elongation in the elongation zone. The boundary between dividing and elongating cells is called the transition zone. In the meristem zone, initial cells are continuously dividing, but on the basal side of the meristem cells exit the meristem through the transition zone and enter in the elongation zone, where they stop division and rapidly elongate. Throughout this journey cells are accompanied by changes in cell cycle progression. Flow cytometry analysis showed that meristematic cells are in cycle, but exit when they enter the elongation zone. In addition, the percentage of cells in G2 phase (4C) strongly increased from the meristem to the elongation zone. However, we did not observe remarkable changes in the percentage of cells in cell cycle phases along the entire elongation zone. These results suggest that meristematic cells in maize root apex stop the cell cycle in G2 phase after leaving the meristem.

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Section: Resultsmentioning

confidence: 99%

“…It has been reported that pericycle cells remain in G1-phase until they re-enter the cell cycle ( Vanneste et al, 2007 ). However, pericycle cells represent just a small fraction of the total amount of cells that form the root tips; epidermal and cortex cells being the most abundant types of cell in root apex.…”

Section: Resultsmentioning

confidence: 99%

Transition zone cells reach G2 phase before initiating elongation in maize root apex

Alarcón

Salguero

2017

Biology Open

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“…Also, dephosphorylation of CDKs at G2/M occurs in response to a cytokinin signal (Zhang et al, 1996(Zhang et al, , 2005Orchard et al, 2005) followed by entry of cells into mitosis. Likewise, in auxin-treated roots, two positive G1/S regulators, D-type cyclins and E2F transcription factors, were upregulated whereas two negative regulators, KRP1 and 2, were downregulated (Himanen et al, 2002;Vanneste et al, 2007). Moreover, the latter authors suggested that an A-type cyclin (Arath;CYCA2;4) was a primary recipient of an auxin signal.…”

Section: Introductionmentioning

confidence: 98%

“…Whereas CDKA;1 is required for both the G1/S and the G2/M transitions, the B-types function only at G2/M (Ferreira et al, 1991;Mironov et al, 1999). In Arabidopis, CDKA;1 expression is linked to meristematic competence in the pericycle (Hemerly et al, 1993) whilst a B-type CDK (CDKB1;1) is most strongly expressed in pericycle cells that initiate lateral root primordia (Beeckman et al, 2001;Vanneste et al, 2007). The RAM of Arabidopsis conforms to a three-tiered closed meristem in which there is a distinct boundary between the epidermis and root cap.…”

Section: Introductionmentioning

confidence: 99%

Differential spatial expression of A- and B-type CDKs, and distribution of auxins and cytokinins in the open transverse root apical meristem of Cucurbita maxima

et al. 2010

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Cucma;CDKA;1 was expressed most strongly in cells that have proliferative competence whereas Cucma;CDKB2 was confined mainly to mitotic cells. iPA and t-Z marked differentiated cells in the RAM, consistent with the known effect of cytokinins in promoting differentiation in root systems. iPA/t-Z were distributed in a converse pattern to Cucma;CDKB2 expression whereas IAA was detected in most cells in the RAM regardless of their proliferative potential.

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“…However, the direct effect of alloxan on cell cycle in plants has not been investigated yet. Majority of investigations of connections between cell cycle progression and ROS were performed either on cell suspension [28] or on whole plant level [21] when cell cycle progression is dependent from redistribution of phytohormone auxin [29]. Much less attention has been paid on an investigation of the link between cell physiological status, chromatin accessibility, and cell cycle activation during cell reprogramming.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress inducing agents’ copper and alloxan accelerate cell cycle re-entering of somatic plant cells in the presence of suboptimal exogenous auxin

Pasternak

2020

Preprint

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The physiological status of differentiated somatic plant cells and kinetics of re-entering in cell cycle were investigated in the case of Medicago sativa leaf protoplasts after the application of oxidative stress-inducing agents. Excess copper (30 μM) and alloxan (0.5 mM) accelerated cell cycle re-entry at an exogenous auxin concentration that alone was insufficient to induce cell activation. Application of stress-inducing agents accelerated changes in the nuclei landscape with further faster re-entry in DNA replication and cytokinesis. This acceleration was accompanied by a lower level of reactive oxygen species (ROS) accumulations. At later stages, stress-agents treated cells resemble stem cells in planta with a smaller size, higher cell viability, lower ROS level, and lower activities of major ROS scavenging enzymes. A similar cellular response could be achieved by increasing the exogenous auxin concentration. Based on these experimental results, it is suggested that sub-lethal stress treatments evoke a transient cell state that accelerates cellular reprogramming. We also speculate that this transient cell state serves as an effective mechanism for protection against oxidative stress.

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Auxin Fuels the Cell Cycle Engine During Lateral Root Initiation

Cited by 3 publications

References 94 publications

Transition zone cells reach G2 phase before initiating elongation in maize root apex

Transition zone cells reach G2 phase before initiating elongation in maize root apex

Differential spatial expression of A- and B-type CDKs, and distribution of auxins and cytokinins in the open transverse root apical meristem of Cucurbita maxima

Oxidative stress inducing agents’ copper and alloxan accelerate cell cycle re-entering of somatic plant cells in the presence of suboptimal exogenous auxin

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