Auxin EvoDevo: Conservation and Diversification of Genes Regulating Auxin Biosynthesis, Transport, and Signaling

Matthes, Michaela Sylvia; Best, Norman B.; Robil, Janlo M; Malcomber, Simon T.; Gallavotti, Andrea; McSteen, Paula

doi:10.1016/j.molp.2018.12.012

Cited by 121 publications

(101 citation statements)

References 236 publications

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“…Relative expression analysis showed that these genes were transcriptionally elevated in transgenic Arabidopsis plants, suggesting that they may be targets of TaSNAC8‐6A (Figure e). While in TaSNAC8‐6A transgenic wheat lines, root transcriptome analysis revealed significant up‐regulation of several auxin transporter genes and the auxin polar transport pathway were highly enriched (Figure c; Figure S9c; Table S6), in agreement with the known mechanisms of auxin translocation (Lavenus et al , ; Matthes et al , ). Our data also revealed similar up‐regulation of several wheat homologs of canonical auxin signal transduction genes, such as ARFs, EINs and Aux/IAA (Figure c; Figure S9c; Table S6).…”

Section: Discussionsupporting

confidence: 64%

Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings

Mao

Wang

et al. 2019

Plant Biotechnology Journal

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Summary Wheat is a staple crop produced in arid and semi‐arid areas worldwide, and its production is frequently compromised by water scarcity. Thus, increased drought tolerance is a priority objective for wheat breeding programmes, and among their targets, the NAC transcription factors have been demonstrated to contribute to plant drought response. However, natural sequence variations in these genes have been largely unexplored for their potential roles in drought tolerance. Here, we conducted a candidate gene association analysis of the stress‐responsive NAC gene subfamily in a wheat panel consisting of 700 varieties collected worldwide. We identified a drought responsive gene, TaSNAC8‐6A, that is tightly associated with drought tolerance in wheat seedlings. Further analysis found that a favourable allele TaSNAC8‐6AIn‐313, carrying an insertion in the ABRE promoter motif, is targeted by TaABFs and confers enhanced drought‐inducible expression of TaSNAC8‐6A in drought‐tolerant genotypes. Transgenic wheat and Arabidopsis TaSNAC8‐6A overexpression lines exhibited enhanced drought tolerance through induction of auxin‐ and drought‐response pathways, confirmed by transcriptomic analysis, that stimulated lateral root development, subsequently improving water‐use efficiency. Taken together, our findings reveal that natural variation in TaSNAC8‐6A and specifically the TaSNAC8‐6AIn‐313 allele strongly contribute to wheat drought tolerance and thus represent a valuable genetic resource for improvement of drought‐tolerant germplasm for wheat production.

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Section: Discussionsupporting

confidence: 64%

Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings

Mao

Wang

et al. 2019

Plant Biotechnology Journal

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“…3b and 4b). This result could explain why shoot regeneration from stems occurred in medium supplemented only with BA [18,19]. However, the addition of auxin into the media greatly decreased bud regeneration.…”

Section: Discussionmentioning

confidence: 99%

An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants

Zhang

Bozorov

et al. 2020

Plant Methods

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Background: Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations of hormones on wild apple regeneration from leaf and stem explants to establish an optimal regeneration system. Results:Leaves and stems derived from seedlings were cultured on several media supplemented with various concentrations of thidiazuron (TDZ) or 6-benzylaminopurine (BA) in different combinations with 1-naphthaleneacetic acid (NAA). The results showed that the most efficient shoot formation media (35% and 90%) were MS medium containing 4.0 mg L −1 TDZ and 1.0 mg L −1 NAA for leaf explants and MS medium containing 1.0 mg L −1 BA without NAA for stem explant. MS medium supplemented with 0.4 mg L −1 BA and 0.1 mg L −1 NAA (for shoot multiplication) and 1/2 MS + 0.1 mg L −1 NAA + 1.5% sucrose (for rooting) were effective media. Shoot regeneration from leaf explants was the most effective when the explants were placed abaxial side down onto the medium and were subjected to a pre-treatment of 3 weeks in darkness.Conclusions: An optimized regeneration system for M. sieversii that allowed regeneration within 2-3 months developed. The protocol developed herein can be used in large-scale clonal propagation for the conservation of wild apple, M. sieversii.

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“…Nine clades of AUX/IAA proteins have been identified (Matthes et al, 2019), allowing for significant subfunctionalization during the evolution of the AUX/IAA family. The majority of Arabidopsis Aux/IAA mutants were first described by their agravitopric root, reduced lateral roots, and/or shorter root phenotypes (Fukaki et al, 2002; Nagpal et al, 2000; Rouse et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

TheHoja loca1Mutant and AUX/IAA Function in Phytomer Development

Richardson

Sluis

Hake

2020

Preprint

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Auxin plays a vital role in plant organ development, influencing organ initiation and patterning across all axes. The diversity in auxin patterning results from changes in the activities and expression of auxin signaling components, including the AUX/IAA repressors. Higher land plants have multigene AUX/IAA families, which leads to functional redundancy and a lack of phenotype in loss of function mutants. Instead, dominant mutations, which prevent AUX/IAA degradation in response to auxin, have highlighted the importance of these proteins in organ development. Here we report a new dominant AUX/IAA mutant in maize, Hoja loca1 (Oja). Oja has a mutation in the degron motif of ZmIAA28 and affects aerial organ initiation and medio-lateral patterning in the leaf. These phenotypes contrast with other maize AUX/IAA mutants that affect the root or inflorescence only. Oja illustrates the role of auxin signaling in the tight coordination of phytomer unit development and provides evidence of species-specific sub-functionalization of the AUX/IAAs.One Line SummaryThe maize AUX/IAA ZmIAA38 is involved in phytomer coordination, aerial organ initiation and mediolateral patterning, and illustrates species specific sub-functionalization of the AUX/IAAs.

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Auxin EvoDevo: Conservation and Diversification of Genes Regulating Auxin Biosynthesis, Transport, and Signaling

Cited by 121 publications

References 236 publications

Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings

Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings

An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants

TheHoja loca1Mutant and AUX/IAA Function in Phytomer Development

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