An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants

Abstract

Background: Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations of hormones on wild apple regeneration from leaf and stem explants to establish an optimal regeneration system. Results:Leaves and stems derived from seedlings were cultured on several media supplemented with various concentrations of thidiazuron (TDZ) or 6-benzylaminopurine (BA) in different combinations with 1-naphthaleneacetic acid (NAA). The results showed that the most efficient shoot formation media (35% and 90%) were MS medium containing 4.0 mg L −1 TDZ and 1.0 mg L −1 NAA for leaf explants and MS medium containing 1.0 mg L −1 BA without NAA for stem explant. MS medium supplemented with 0.4 mg L −1 BA and 0.1 mg L −1 NAA (for shoot multiplication) and 1/2 MS + 0.1 mg L −1 NAA + 1.5% sucrose (for rooting) were effective media. Shoot regeneration from leaf explants was the most effective when the explants were placed abaxial side down onto the medium and were subjected to a pre-treatment of 3 weeks in darkness.Conclusions: An optimized regeneration system for M. sieversii that allowed regeneration within 2-3 months developed. The protocol developed herein can be used in large-scale clonal propagation for the conservation of wild apple, M. sieversii.

Influence of Culture Media and Explants on in vitro Regeneration Efficiency of Elite Guava Cultivars

“…In the earlier reports, it was found that shoot bud induction and plant regeneration was depends on genotypic variations and medium composition and in guava cultivars (Beaumont, Ka Hua Kula and Lucknow-49), Joshee et al, (2004) reported the same results. Our results suggested that explants either node or shoot tip, regeneration efficiency mainly depends on the growth regulator interactions and cytokinin to auxin ratio (Zhang et al, 2020).…”

“…Any part of the plants could be used as an explant source, but the type of explant has a prime importance in their response to tissue culture for initiation, growth and regeneration potential (Kumar et al, 2014) as well as the composition of culture medium is also an important factor for successful establishment of tissue culture plantlets (Zhang et al, 2020). In the present study, percent regeneration efficiency was calculated as percent shoot induction x mean no.…”

The Effects of Different Sucrose Concentrations on The Regeneration Area of Riccia Fluitans L., A Medicinal Aquatic Plant

“…Plant production is increasing day by day with tissue culture techniques. Many plant species such as Bacopa monnieri L. Pennell [16], Cannabis sativa L. [17] and Malus sieversii [18] have been successfully produced with this method.…”

Antimicrobial and In-vitro Cytotoxic Efficacy of Betulinic Acid from the Callus, Regeneration, and Mother Plant of Dillenia indica L.

“…Islam et al (2017) reported that the efficiency of shoot bud formation was relied upon and enhanced by supplementing the medium with BA. Similarly, Miri (2020) reported that the increase in BA concentration boosted the induction of axillary shoots of Zingiber officinale Rosc Furthermore, (Zhang et al, 2020) concluded that stem explant which grows on an MS medium containing 1.0mg L -1 BA without NAA giving high shoot multiplication to Malus sieversii.…”