Autotrophic and symbiotic diazotrophs dominate nitrogen-fixing communities in Tibetan grassland soils

Che, Rongxiao; Deng, Yangfan; Wang, Fang; Wang, Weijin; Xu, Zhihong; Hao, Yanbin; Xue, Kai; Zhang, Biao; Tang, Lee; Zhou, Huakun; Cui, Xiaoyong

doi:10.1016/j.scitotenv.2018.05.238

Cited by 98 publications

(60 citation statements)

References 85 publications

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“…Differently, the copy number of nifH increased with precipitation almost along the full range of natural precipitation gradient of this study. Consistently, nifH was observed to increase continuously on Qinghai-Tibet Plateau along a natural precipitation gradient ranged from 62 to 614 mm [11]. The accelerated increasing trend of nosZ copy number after its breaking point of 367 mm likely implied a stimulating effect of intensified anaerobic condition [56].…”

Section: Discussionmentioning

confidence: 79%

“…N gene community measured by real-time quantitative PCR (qPCR)at a landscape scale in Burgundy of France [9], by GeoChip in five oil-contaminated fields in China (660-2 030 km apart) [10], and diazotroph communities measured by qPCR and MiSeq sequencing at a regional scale on the Tibetan Plateau, China [11]. Various factors were discovered to influence spatial patterns of microbial N genes, including geographic [10], topographic and hydrological properties [12], as well as soil geochemical parameters [10,11], e.g. pH [9,10] and oil pollution [10].…”

Section: Introductionmentioning

confidence: 99%

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Spatial patterns of microbial nitrogen genes along precipitation gradient in different temperate grasslands of Inner Mongolia, China

Zhou¹,

Xue²,

Zhang³

et al. 2020

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Background: Despite the importance of microorganisms in soil nitrogen (N) cycling, studies on spatial patterns of microbial N genes in the temperate grassland are still lacking, whose productivity is limited by N. Here, we investigated microbial N genes from 60 temperate grassland sites across 1 161 km in Inner Mongolia, China.Results: All N gene abundances tended to decrease from northeast to southwest, consistent with precipitation change but contrary to temperature trend. Most N gene abundances increased with rising precipitation when < 321 or 403 mm, but remained stable after breaking points, indicating nonlinear saturation curves dominated response patterns. Moreover, decay relationships were discovered for N gene community over geographic distance, whose effect was direct in temperate desert steppe but indirect via environmental heterogeneity in temperate meadow and typical steppe.Representativeness of geographic distance on historical-contingency was dominant only in temperate meadow (81.2%). N gene community similarity decay was mainly attributed to plant community (76.98%), with wider range, in typical steppe; while contemporary-disturbance was the attributor more important in temperate meadow (29.41%).Conclusions: Overall, we discovered non-linear patterns of N genes along precipitation gradient, and quantified attributions of geographic distance, plant community, historical-contingency and contemporary-disturbance to N gene community similarity decay, clearly ecosystem-dependent.Not applicable. Consent for publicationNot applicable.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Spatial patterns of microbial nitrogen genes along precipitation gradient in different temperate grasslands of Inner Mongolia, China

Zhou¹,

Xue²,

Zhang³

et al. 2020

Preprint

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“…In previous studies, the fixation of inorganic carbon and nitrogen by such microorganisms was recognized as a crucial process to the development of life in extreme environments [72,73]. In the G3 sediments, autotrophic microbial communities were dominated by diazotrophs, which was also found in some Tibetan soils [74], and the reason for this phenomenon is still mysterious. Dissimilatory nitrate reduction pathway was detected in a few MAGs affiliated with the Betaproteobacteria (n = 7), Deltaproteobacteria (n = 2) and Gammaproteobacteria (n = 1), with the highest species number and fraction in the freshwater G1 sites (Fig.…”

Section: Autotrophic Nitrogen-cycling Capabilities Along a Salinity Gmentioning

confidence: 98%

Salinity-triggered compositional and metabolic responses of autotrophic prokaryotes in Tibetan lacustrine sediments

Li¹,

Yuan²,

Yang³

et al. 2020

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Background Autotrophic prokaryotes are crucial participants in the global carbon cycle, and autotrophic carbon fixation contributes approximately 50% of the global net primary production in aquatic ecosystems per year. Salinity is a prominent regulator structuring microbial communities in diverse aquatic ecosystems. However, little information is available regarding the compositional and physiological response of autotrophic microbial communities to salinity change. Here, we used genome-resolved metagenomics to study autotrophic microbial communities in 25 Tibetan lacustrine sediments with a salinity gradient (from 0.54‰ to 82.6‰). Results117 metagenome-assembled genomes (MAGs) with carbon fixation potential belonging to 12 phyla were retrieved, of which approximately 21% were not affiliated with the known orders, suggesting taxonomically diverse autotrophic assemblages in sediments. The total abundance of these putative autotrophs decreased significantly with increasing salinity, and the variation of sediment autotrophic communities was mainly driven by salinity, pH and TOC. Notably, a change in the predominant lineage from Betaproteobacteria to Deltaproteobacteria was observed along the salinity gradient, and the dominant pathway for carbon fixation shifted from the Calvin-Benson-Bassham (CBB) cycle to more energy efficient Wood-Lungdahl (WL) pathway with glycolysis from Entner-Doudoroff to more exergonic Embden-Meyerhof-Parnas, demonstrating that the physiological efficiency increases from freshwater to hypersaline autotrophic communities. Metabolic inference revealed major links for carbon fixation to the oxidation of reduced sulfur compounds, ferrous iron and carbon monoxide, denitrification and nitrogen fixation in these MAGs, as well as the occurrence of dissimilatory sulfate reduction and the WL pathway dominating hypersaline sediments, greatly extending the understanding of metabolic versatility and diverse ecological niches of autotrophic microorganisms. Conclusions This study provided a systematic attempt to characterize the response of carbon fixation pathways to salinity and the knowledge essential for revealing ecological roles of autotrophic prokaryotes in aquatic habitats. These findings suggest with increased salinity, physiological efficiency of the autotrophic community increases, which has important implications for understanding the carbon budget in aquatic ecosystems.

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“…土壤pH和降水等因子被发现同青藏高原土壤细菌多样性、群落组成的差异显著相关 [26,27] , 而土壤真菌的多样性与植物多样性显著相关 [28] . 此外, 固氮菌的多样性被发现受生态系统类型的影响 [29] , 而优势植物根际AMF(arbuscular mycorrhizal fungi)群落的物种丰富度主要受降水量的影响 [30] . 达27%.…”

Section: 作为地球的第三极青藏高原是气候和生态环境unclassified

Soil microbial communities in alpine grasslands on the Tibet Plateau and their influencing factors

et al. 2019

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Autotrophic and symbiotic diazotrophs dominate nitrogen-fixing communities in Tibetan grassland soils

Cited by 98 publications

References 85 publications

Spatial patterns of microbial nitrogen genes along precipitation gradient in different temperate grasslands of Inner Mongolia, China

Spatial patterns of microbial nitrogen genes along precipitation gradient in different temperate grasslands of Inner Mongolia, China

Salinity-triggered compositional and metabolic responses of autotrophic prokaryotes in Tibetan lacustrine sediments

Soil microbial communities in alpine grasslands on the Tibet Plateau and their influencing factors

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