Autospecific and allospecific antibodies raised in allotype suppressed rabbits

Dubiski, S.; Good, P. W.

doi:10.1016/0161-5890(79)90032-4

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…That this inhibition of secretion can be abrogated, at least in vitro, has been shown experimentally, and this finding has been interpreted in terms of interference with an active suppression mechanism (3,4). Finally, ~leliberate immunization of thoroughly suppressed rabbits with their own suppressed type Ig has been shown to lead to auto-antibody formation, indicating that the potential for an auto-anti-allotypic response exists in such animals (5)(6)(7).…”

Section: From the Division Of Immunology St Jude Children's Research Hospital Memphis Tennessee 38101; And The Center For Genetics And Thmentioning

confidence: 97%

Induction of lymphoid cell chimerism in noninbred, histocompatible rabbits. A new model for studying allotype suppression in the rabbit.

Adler¹,

Adler²,

Cohen³

et al. 1981

The Journal of Experimental Medicine

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Noninbred rabbits, matched with regard to the major histocompatibility complex (RLA-A and RLA-D loci) but mismatched for Ig allotypes, served as donors (adult) and recipients (newborn) of lymphoid cells. Lasting chimerism regularly followed the transfer of 1 x 10(8)-3 x 10(8) spleen, lymph node, or bone marrow cells, as indicated by the continued production of Ig with allotypic determinants of both donor and recipient. Typically, Ig of donor allotype accounted for 25-50% of total allotypic Ig at 4 wk of age and the amount of donor Ig produced remained stable for up to 20 mo. Total allotypic Ig levels remained normal in the chimeric rabbits. "Chimeric drift" or a gradual diminution of donor products over a period of several months, occurred in some individuals. Transfer of lymphoid cells from allotype-suppressed adult donors to newborns of appropriate allotypes did not result in specific suppression of the target allotype in the recipients. Other experiments showed that lymphoid cells from suppressed donors adoptively transferred to histocompatible recipients continued to synthesize Ig of the nonsuppressed type only. The suitability of using an outbred population of histocompatible but allotype-mismatched rabbits for analyzing allotype suppression and other immunoregulatory phenomena is demonstrated by the results presented here.

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Section: From the Division Of Immunology St Jude Children's Research Hospital Memphis Tennessee 38101; And The Center For Genetics And Thmentioning

confidence: 97%

Induction of lymphoid cell chimerism in noninbred, histocompatible rabbits. A new model for studying allotype suppression in the rabbit.

Adler¹,

Adler²,

Cohen³

et al. 1981

The Journal of Experimental Medicine

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Allotypes in Basilea rabbits

Weiß¹,

García

Jaton³

et al. 1982

Eur J Immunol

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Basilea rabbits produce immunoglobulin molecules, practically all of which have lambda light chains rather than kappa chains. This variant strain was derived form a homozygous (b9/b9) male. Sensitive serological methods revealed that at least some homozygous bas/bas individuals possess traces (about 100 ng/ml) of b9 molecules. This level usually increases to almost 1 microgram/ml upon hyperimmunization with pneumococcal or streptococcal vaccines. One exceptional rabbit, with 50 micrograms/ml of b9 molecules, was found. In spite of the presence of b9 molecules in early pre-immune bleeds, homozygous bas/bas rabbits produce strong anti-b9 antibodies; i.e., they are capable of making autoantibodies. These anti-b9 allotypic antisera were not distinguishable by our methods from routinely produced anti-b9.

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Production of IgG‐specific auto‐anti‐allotypes in b4.2‐suppressed rabbits

et al. 1982

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Rabbits were completely suppressed for kappa chain allotype b4.2, and autoantibodies against b4.1 or b4.2 could be raised in 2 out of 3 animals. The animal immunized with b4.1 produced anti-b4 and anti-Ms3, two activities which have never as yet been found together in one antiserum. Both autoantisera lacked the capacity to bind b4.2-IgM, whereas they precipitated 4.2-IgG very well. In one animal, anti-b4 IgM activity appeared after the sixth immunization, at the age of 14 months. Allotype suppression was maintained in both rabbits till the end of their lives whereas all control animals recovered within 6 months. The autoantisera which were specific for b4 IgG could not induce suppression in vivo. However, specific inhibition of b4 IgG secretion was observed in vitro.

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Autospecific and allospecific antibodies raised in allotype suppressed rabbits

Cited by 6 publications

References 16 publications

Induction of lymphoid cell chimerism in noninbred, histocompatible rabbits. A new model for studying allotype suppression in the rabbit.

Induction of lymphoid cell chimerism in noninbred, histocompatible rabbits. A new model for studying allotype suppression in the rabbit.

Allotypes in Basilea rabbits

Production of IgG‐specific auto‐anti‐allotypes in b4.2‐suppressed rabbits

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