Autosomal Interactions and Mechanisms of Pyrethroid Resistance in House Flies, <i>Musca domestica</i>

Tian, Li; Cao, Chuanwang; He, Lin; Li, Ming; Zhang, Lan; Zhang, Lee; Liu, Huqi; Liu, Nannan

doi:10.7150/ijbs.7.902

Cited by 26 publications

(28 citation statements)

References 51 publications

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“…Since pyrethroids and organophosphates have different modes of action, their mixtures have commonly been in practice against a variety of pests worldwide for the last many years [8]. Increased metabolic detoxification is one of the major mechanisms in house flies for the development of insecticide resistance [15]. Previously it has been assumed that organophosphates, when used in combination with pyrethroids, inhibit the enzymes responsible for metabolic detoxification in different insect pests [6], [9], [10].…”

Section: Introductionmentioning

confidence: 99%

Insecticide Mixtures Could Enhance the Toxicity of Insecticides in a Resistant Dairy Population of Musca domestica L

et al. 2013

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House flies, Musca domestica L., are important pests of dairy operations worldwide, with the ability to adapt wide range of environmental conditions. There are a number of insecticides used for their management, but development of resistance is a serious problem. Insecticide mixtures could enhance the toxicity of insecticides in resistant insect pests, thus resulting as a potential resistance management tool. The toxicity of bifenthrin, cypermethrin, deltamethrin, chlorpyrifos, profenofos, emamectin benzoate and fipronil were assessed separately, and in mixtures against house flies. A field-collected population was significantly resistant to all the insecticides under investigation when compared with a laboratory susceptible strain. Most of the insecticide mixtures like one pyrethroid with other compounds evaluated under two conditions (1∶1-“A” and LC50: LC50-“B”) significantly increased the toxicity of pyrethroids in the field population. Under both conditions, the combination indices of pyrethroids with other compounds, in most of the cases, were significantly below 1, suggesting synergism. The enzyme inhibitors, PBO and DEF, when used in combination with insecticides against the resistant population, toxicities of bifenthrin, cypermethrin, deltamethrin and emamectin were significantly increased, suggesting esterase and monooxygenase based resistance mechanism. The toxicities of bifenthrin, cypermethrin and deltamethrin in the resistant population of house flies could be enhanced by the combination with chlorpyrifos, profenofos, emamectin and fipronil. The findings of the present study might have practical significance for resistance management in house flies.

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Section: Introductionmentioning

confidence: 99%

Insecticide Mixtures Could Enhance the Toxicity of Insecticides in a Resistant Dairy Population of Musca domestica L

et al. 2013

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“…Increased metabolic detoxification and target-site insensitivity are the two major mechanisms involved in insecticide resistance development in M. domestica. The microsomal oxidases, hydrolases, and glutathione S-transferases are major enzyme families involved in metabolic detoxification of insecticides [25]. Metabolic detoxification was known to be involved in pyriproxyfen resistance in B. tabaci [17,18] and M. domestica [26].…”

Section: Introductionmentioning

confidence: 99%

Mechanism, stability and fitness cost of resistance to pyriproxyfen in the house fly, Musca domestica L. (Diptera: Muscidae)

Shah

Shad

Abbas

2015

Pesticide Biochemistry and Physiology

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“…Genetic studies have linked pyrethroid resistance to autosomes 1, 2, 3 and 5 [5,30]. The major mechanisms governing pyrethroid resistance in this strain include increased detoxification mediated by P450 monooxygenases and decreased sensitivity of voltage-gated sodium channels ( kdr ) [15,31,32].…”

Section: Introductionmentioning

confidence: 99%

A whole transcriptomal linkage analysis of gene co-regulation in insecticide resistant house flies, Musca domestica

Reid

Zhang

et al. 2013

BMC Genomics

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BackgroundStudies suggest that not only is insecticide resistance conferred via multiple gene up-regulation, but it is mediated through the interaction of regulatory factors. However, no regulatory factors in insecticide resistance have yet been identified, and there has been no examination of the regulatory interaction of resistance genes. Our current study generated the first reference transcriptome from the adult house fly and conducted a whole transcriptome analysis for the multiple insecticide resistant strain ALHF (wild-type) and two insecticide susceptible strains: aabys (with morphological recessive markers) and CS (wild type) to gain valuable insights into the gene interaction and complex regulation in insecticide resistance of house flies, Musca domestica.ResultsOver 56 million reads were used to assemble the adult female M. domestica transcriptome reference and 14488 contigs were generated from the de novo transcriptome assembly. A total of 6159 (43%) of the contigs contained coding regions, among which 1316 genes were identified as being co-up-regulated in ALHF in comparison to both aabys and CS. The majority of these up-regulated genes fell within the SCOP categories of metabolism, general, intra-cellular processes, and regulation, and covered three key detailed function categories: redox detailed function category in metabolism, signal transduction and kinases/phosphatases in regulation, and proteases in intra-cellular processes. The redox group contained detoxification gene superfamilies, including cytochrome P450s, glutathione S-transferases, and esterases. The signal transduction and kinases/phosphatases groups contained gene families of rhodopsin-like GPCRs, adenylate and guanylate cyclases, protein kinases and phosphatases. The proteases group contained genes with digestive, catalytic, and proteinase activities. Genetic linkage analysis with house fly lines comparing different autosomal combinations from ALHF revealed that the up-regulation of gene expression in the three key SCOP detailed function categories occurred mainly through the co-regulation of factors among multiple autosomes, especially between autosomes 2 and 5, suggesting that signaling transduction cascades controlled by GPCRs, protein kinase/phosphates and proteases may be involved in the regulation of resistance P450 gene regulation.ConclusionTaken together, our findings suggested that not only is insecticide resistance conferred via multi-resistance mechanisms or up-regulated genes, but it is mediated through the trans and/or cis co-regulations of resistance genes.

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Autosomal Interactions and Mechanisms of Pyrethroid Resistance in House Flies, Musca domestica

Cited by 26 publications

References 51 publications

Insecticide Mixtures Could Enhance the Toxicity of Insecticides in a Resistant Dairy Population of Musca domestica L

Insecticide Mixtures Could Enhance the Toxicity of Insecticides in a Resistant Dairy Population of Musca domestica L

Mechanism, stability and fitness cost of resistance to pyriproxyfen in the house fly, Musca domestica L. (Diptera: Muscidae)

A whole transcriptomal linkage analysis of gene co-regulation in insecticide resistant house flies, Musca domestica

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