Autoradiographische untersuchungen zur frage des transfers markierter RNS von makrophagen auf lymphocyten

Jonas, Ludwig; P, Dörfling; M, Barten; Tessmann, D

doi:10.1016/s0065-1281(76)80117-1

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…We suspect that similar cell-cell interaction may permit transfer of influenza virus RNA from macrophage to lymphocyte, either directly via the broad base observed in macrophage-lymphocyte clusters (20), or indirectly via macrophage endocytosis of virus followed by as yet undefined internal handling events (23), recycling of part or all of the virus genome to the cell surface, and subsequent transfer to the adjoining lymphocyte, possibly via endocytosis by the lymphocyte of its own histocompatibility class II (24) and virus-occupied influenza virus receptor. Others have shown, by electron microscopic autoradiography, transfer of radiolabeled RNA from normal murine macrophages to autologous lymphocytes, with transfer enhanced by exposure of the lymphocytes to endotoxin-stimulated radiolabeled macrophages (25). The current data do not exclude the possibility that cell-to-cell transfer of influenza virus does not occur, but that virus infection of lymphocytes is the result of direct infection of the lymphocyte, which is facilitated in some other way by the presence of adjacent macrophages.…”

Section: Discussioncontrasting

confidence: 79%

Macrophages are required for influenza virus infection of human lymphocytes.

Mock¹,

Domurat²,

Roberts³

et al. 1987

J. Clin. Invest.

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Monocyte and lymphocyte surface-expressed viral antigens have been demonstrated after exposure of unseparated human mononuclear leukocytes to influenza virus in vitro. The current studies, using [35S]methionine pulse-labeled purified preparations of virus-exposed macrophages, depleted of lymphocytes, demonstrate that the presence of these viral proteins does represent new synthesis. However, purified lymphocytes, depleted of monocytes-macrophages and exposed to influenza virus, showed no detectable viral protein synthesis. In further experiments, unseparated mononuclear leukocytes were exposed to virus and subsequently separated by countercurrent centrifugal elutriation. Both macrophages and lymphocytes were then shown to synthesize influenza proteins. Cell-free control or influenza virus-infected macrophage-derived supernatant fluids did not facilitate influenza virus infection of the lymphocytes. The data suggest that macrophages are required for influenza virus infection of human lymphocytes, and raise the possibility that macrophage facilitation of an abortive infection of lymphocytes plays a role in the generation of effective immunity to viral antigens.

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Section: Discussioncontrasting

confidence: 79%

Macrophages are required for influenza virus infection of human lymphocytes.

Mock¹,

Domurat²,

Roberts³

et al. 1987

J. Clin. Invest.

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Macrophage-lymphocyte cluster formation in the medullary sinus of lymph node after immunization with sheep red blood cells (SRBC)

1977

Cell Tissue Res.

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Normally the lymphatic sinuses of the lymph node are loosely packed with lymphocytes and free macrophages as well as with macrophages adhering to the fibrocellular trabeculae. After immunization with SRBC cluster formation occurs in the medullary sinuses of rats between a central macrophage and peripherally located lymphocytes. These rosette-like clusters are nearly identical with the clusters found during primary and secondary immune response against SRBC in vitro and seem to be the in vivo equivalent for the same immune response.

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Lysosomal Functions in Cellular Activation: Propagation of the Actions of Hormones and Other Effectors

Szego

Pietras

1984

International Review of Cytology

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Autoradiographische untersuchungen zur frage des transfers markierter RNS von makrophagen auf lymphocyten

Cited by 3 publications

References 12 publications

Macrophages are required for influenza virus infection of human lymphocytes.

Macrophages are required for influenza virus infection of human lymphocytes.

Macrophage-lymphocyte cluster formation in the medullary sinus of lymph node after immunization with sheep red blood cells (SRBC)

Lysosomal Functions in Cellular Activation: Propagation of the Actions of Hormones and Other Effectors

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