Autoradiographische Untersuchungen der DNS- und Eiwei�-Neubildung im gyn�kologischen Untersuchungsmaterial

Fettig, O; Oehlert, W.

doi:10.1007/bf00668044

Cited by 22 publications

(4 citation statements)

References 16 publications

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“…Hyaluronidase/ml InkubationslSsung) [9,10]. Zur Verbesserung der Eindringtiefe der radioaktiven Substanz in das Placentagewebe wurde der jeweiligen InkubationslSsung Hyaluronidase hinzugegeben (50 i.E.…”

Section: Materials Und Methodeunclassified

“…])urch den Hyaluronidase-Zusatz und durch den Schfittelvorgang wurde der Kontakt der Gewebsober-fl~che mit dem :[nkubationsmedium verbessert und die Eindringtiefe yon 100 ~ [17] auf 400--530 Ez [9] erhSht. ])urch den Hyaluronidase-Zusatz und durch den Schfittelvorgang wurde der Kontakt der Gewebsober-fl~che mit dem :[nkubationsmedium verbessert und die Eindringtiefe yon 100 ~ [17] auf 400--530 Ez [9] erhSht.…”

Section: Materials Und Methodeunclassified

“…Wir benutzten hierzu die autoradiographische Inkubationsmethode [9,26], da frfihere Untersuchungen an anderen Geweben gezeigt haben, dab die in vitro-Inkubation yon malignen und benignen Geweben mit Tritium markierten Thymidin Aufschlu$ fiber die DNS-Synthese yon Geweben unter in vivo-Bedingungen gibt [9,10,28,31].…”

Section: Introductionunclassified

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Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions

1974

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Section: Materials Und Methodeunclassified

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Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions

1974

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“…Radio¬ active uptake is seen only in the periphery of the specimens, labelling depth does not usually exceed 100-200/¿m. Fettig & Oehlert (1964a) increased the penetration of isotopes to 400-500 /¿m by means of hyaluronidase. Excess oxygen pressure (2 atmospheres) has accordingly been used (Steel & Bensted, 1965).…”

Section: Discussionmentioning

confidence: 99%

The Synthesis of Dna and Rna in Normal Human Endometrium in Short-Term Incubation in Vitro and Its Response to Oestradiol and Progesterone

Nordqvist¹

1970

Journal of Endocrinology

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A method is described for short-term incubations in vitro of normal endometrium for the study of nucleic acid synthesis. Tissue suspensions of specimens obtained at curettage were incubated with and without hormones in a medium consisting of Parker's 199 medium and 20% adult human serum; [3H]thymidine and [14C]uridine were added. The isotope uptake into the nucleic acids was determined and related to the total amount of DNA in each sample. Marked variation in DNA synthesis was noted in endometria obtained at different phases of the menstrual cycle. RNA synthesis varied less. After the addition of progesterone, synthesis of both nucleic acids was reduced. The magnitude of this response varied in different endometria. Thus DNA synthesis in endometria already under strong progesterone influence in vivo (midsecretory phase) was least affected when progesterone was added in vitro.

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Dysplasie — Carcinoma in situ — Mikrocarcinom der Cervix uteri

Hillemanns¹,

Limburg²

1972

Vulva, Vagina, Urethra

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Autoradiographische Untersuchungen der DNS- und Eiwei�-Neubildung im gyn�kologischen Untersuchungsmaterial

Cited by 22 publications

References 16 publications

Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions

Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions

The Synthesis of Dna and Rna in Normal Human Endometrium in Short-Term Incubation in Vitro and Its Response to Oestradiol and Progesterone

Dysplasie — Carcinoma in situ — Mikrocarcinom der Cervix uteri

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