Autoradiographische untersuchung mit [3H]thymidin über die dauer der deoxyribonukleinsäure-synthese und ihren zeitlichen verlauf bei den darmepithelien und anderen zelltypen der maus

Koburg, E.; Maurer, W.

doi:10.1016/0926-6550(62)90085-3

Cited by 31 publications

(15 citation statements)

References 22 publications

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“…The majority of the precursor is already incorporated about 20 min after injection. This agrees well with the availability time of t3H]-TdR measured in mouse jejunal crypt cells by Quastler & Sherman (1959) and Koburg & Maurer (1962). The availability time of TdR doses exceeding the tracer dose does not differ from that of tracer doses ( Fig.…”

Section: Flm Curves Of Tumour Cells In a Xenograft Of A Human Renal Csupporting

confidence: 89%

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

1990

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Abstract. The present experiments with [14C]‐thymidine (TdR) and [3H]‐bromo‐deoxyuridine (BrdU) using mouse jejunal crypt cells show that the upper limit of the tracer dose of TdR is about 0.5 µg g body weight‐1 and that of BrdU is about 5·0 µg g body weight‐1. Applying these doses, the proportions of the endogenous DNA synthesis attributed to the exogenous DNA precursor are 2% and 9% respectively. For [3H]‐TdR doses commonly used in cell kinetic studies this proportion is only 0‐1‐1.0%, a negligible quantity that does not influence the endogenous DNA synthesis. The maximum availability time of tracer doses of TdR as well as BrdU is 40 to 60 min, the majority of the precursors being incorporated after 20 min. The availability time is the same for TdR doses exceeding the tracer dose by a factor of 80, whereas it is prolonged in the case of BrdU doses exceeding the tracer dose by a factor of 50. BrdU is suitable to replace radioactively labelled TdR in short term cell kinetic studies, i.e. determination of the labelling index or of the S phase duration by double labelling. However, more studies are needed to elucidate how far BrdU can replace TdR in long term studies as shown by differences between the fraction of labelled mitoses (FLM) curves of a human renal cell carcinoma measured with BrdU and [3H]‐TdR.

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Section: Flm Curves Of Tumour Cells In a Xenograft Of A Human Renal Csupporting

confidence: 89%

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

1990

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“…Labeled cells were defined as cells having more than five grains over the nucleus detected autoradiographically (14).…”

Section: Methodsmentioning

confidence: 99%

Renewal and migration of rat incisor mesenchymal cells after doxorubicin administration

Dahl

Koppang

1985

Acta Odontologica Scandinavica

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Ten female rats were intravenously injected with doxorubicin (20 mg/kg) and divided into two equal groups receiving a dose of tritiated thymidine either simultaneously with doxorubicin or 1 h before being killed after 5 days. Six control animals were correspondingly injected with 3H-thymidine. The left and right maxillary incisors were prepared for histologic and microautoradiographic investigation, respectively. The distribution of labeled cells in animals injected 1 h before death showed the regions of proliferation, whereas migration from these regions was evaluated by longer observation time. A zone of reduced dentin deposition lined by irregular odontoblasts being young odontoblasts and late preodontoblasts was observed at the time of doxorubicin injection. Pulpally to the dentinal lesion, islands of irregular predentin were deposited by non-progenitive pulp cells and depolarized odontoblasts. The late preodontoblasts were renewed from progenitive pulp cells, leading to a disturbed mantle predentin deposition.

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“…Ts is considered to be equal to the time interval between the 50% points on the ascending and descending limbs of the curve. However, this estimation of Ts does not take into consideration the effective labeling time of 3H-TdR, which is 0.5-0.75 hours in mammals (Koburg and Maurer, 1962;Schultze, 19691, and correspondingly shifts ahead the descending limb of the curve. Therefore, 0.5 hour was subtracted from the Ts values obtained from the graphs.…”

Section: Cell Kinetics In the Percentage Of Labeled Mitoses Experimentsmentioning

confidence: 99%

Variability and partial synchrony of the cell cycle in the germinal zone of the early embryonic cerebral cortex

Reznikov

Kooy

1995

J of Comparative Neurology

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Cell cycle parameters were estimated using the cumulative 3H-thymidine S-phase labeling and percentage of labeled mitoses methods in the embryonic day 14 and 15 germinal zone of the rat cerebral cortex. The shortest cell cycle time was seen in the dorsal neocortex and the longest in the lateral neocortex and fimbria (the latter also had a low growth fraction). No differences were observed in cell cycle times between the cells in the ventricular and subventricular zone in the same neocortical region. The results suggest gradients of lengthening cell cycle times extending ventrolaterally and ventromedially from the dorsomedial neocortex. Although a majority of proliferating cells in individual cortical regions seem to belong to one population in terms of cell kinetics, several pieces of evidence suggest some heterogeneity: the asymmetric shapes of the percentages of labeled mitoses curves, the small population of noncycling neuroepithelial cells in the neocortex and mesocortex, and small population of cells that become pyknotic. Groups of DNA-synthesizing nuclei that were ectopically located in the inner half of the ventricular zone also indicate the existence of different subpopulations of neuroepithelial cells. In addition, after a pulse injection of 3H-thymidine the germinal zone is characterized by alternating clusters of heavily and lightly labeled cell nuclei that may reflect the simultaneous passage of a cluster of cells through the same portion of S-phase. We suggest that partial cell cycle synchrony within groups of ventricular cells may explain the presence of these iterative cell kinetic patterns in the developing cortex.

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Autoradiographische untersuchung mit [3H]thymidin über die dauer der deoxyribonukleinsäure-synthese und ihren zeitlichen verlauf bei den darmepithelien und anderen zelltypen der maus

Cited by 31 publications

References 22 publications

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studies

Renewal and migration of rat incisor mesenchymal cells after doxorubicin administration

Variability and partial synchrony of the cell cycle in the germinal zone of the early embryonic cerebral cortex

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