Autophagy induced by snakehead fish vesiculovirus inhibited its replication in SSN-1 cell line

Wang, Yao; Chen, Nan; Hegazy, Abeer M.; Liu, Xiaodan; Wu, Zhixin; Liu, Xueqin; Zhao, Lijuan; Qin, Qiwei; Lan, Jiang-Feng; Lin, Li

doi:10.1016/j.fsi.2016.06.019

Cited by 26 publications

(7 citation statements)

References 35 publications

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“…Autophagy is a natural, conserved, and self-digestive catabolic process that can be critical for cell survival under stressful conditions, such as viral infection [78,79]. In fish, autophagy has been implicated in viral infections either facilitating [80,81] or inhibiting virus replication [82]. Recently, autophagy has been described in nucleated RBCs as a mechanism for defense against viruses [22,83], and the GVHSV protein is known to be involved in autophagy following immunization with the DNA vaccine [24].…”

Section: Discussionmentioning

confidence: 99%

Potential Role of Rainbow Trout Erythrocytes as Mediators in the Immune Response Induced by a DNA Vaccine in Fish

et al. 2019

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In recent years, fish nucleated red blood cells (RBCs) have been implicated in the response against viral infections. We have demonstrated that rainbow trout RBCs can express the antigen encoded by a DNA vaccine against viral hemorrhagic septicemia virus (VHSV) and mount an immune response to the antigen in vitro. In this manuscript, we show, for the first time, the role of RBCs in the immune response triggered by DNA immunization of rainbow trout with glycoprotein G of VHSV (GVHSV). Transcriptomic and proteomic profiles of RBCs revealed genes and proteins involved in antigen processing and presentation of exogenous peptide antigen via MHC class I, the Fc receptor signaling pathway, the autophagy pathway, and the activation of the innate immune response, among others. On the other hand, GVHSV-transfected RBCs induce specific antibodies against VHSV in the serum of rainbow trout which shows that RBCs expressing a DNA vaccine are able to elicit a humoral response. These results open a new direction in the research of vaccination strategies for fish since rainbow trout RBCs actively participate in the innate and adaptive immune response in DNA vaccination. Based on our findings, we suggest the use of RBCs as target cells or carriers for the future design of novel vaccine strategies.

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Section: Discussionmentioning

confidence: 99%

Potential Role of Rainbow Trout Erythrocytes as Mediators in the Immune Response Induced by a DNA Vaccine in Fish

et al. 2019

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“…In recent years, the relationship between some aquatic viruses and autophagy has gradually been revealed, including viral hemorrhagic septicemia virus, spring viremia of carp virus, snakehead fish vesiculovirus, grouper iridovirus, largemouth bass virus, infectious kidney and spleen necrosis virus, and white spot syndrome virus (WSSV) (Garcia-Valtanen et al, 2014;Liu et al, 2015;Chen et al, 2016;Qi et al, 2016;Wang et al, 2016;Li et al, 2017). Based on current studies, the relationship between viruses and autophagy varies according to the type of virus and the host cell line.…”

Section: Introductionmentioning

confidence: 99%

Singapore Grouper Iridovirus (SGIV) Inhibited Autophagy for Efficient Viral Replication

Wang

Liu

et al. 2020

Front. Microbiol.

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Autophagy is a conserved catabolic process that occurs at basal levels to maintain cellular homeostasis. Most virus infections can alter the autophagy level, which functions as either a pro-viral or antiviral pathway, depending on the virus and host cells. Singapore grouper iridovirus (SGIV) is a novel fish DNA virus that has caused great economic losses for the marine aquaculture industry. In this study, we found that SGIV inhibited autophagy in grouper spleen (GS) cells which was evidenced by the changes of LC3-II, Beclin1 and p-mTOR levels. Further study showed that SGIV developed at least two strategies to inhibit autophagy: (1) increasing the cytoplasmic p53 level; and (2) encoding viral proteins (VP48, VP122, VP132) that competitively bind autophagy related gene 5 and mediately affect LC3 conversion. Moreover, activation of autophagy by rapamycin or overexpressing LC3 decreased SGIV replication. These results provide an antiviral strategy from the perspective of autophagy.

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“…Autophagy is an evolutionarily conserved membrane-trafficking process and maintains the cellular metabolic homeostasis. Besides its role in healthy catabolic processes, many viruses induce autophagy for their own benefit during virus infection, such as VHSV [10], SVCV [11], SHVV [12], RGNNV [25], IPNV [26], rotavirus [27], etc. In this study, we observed that SCRV infection induced a complete autophagy process in CPB cells.…”

Section: Discussionmentioning

confidence: 99%

“…SVCV induced autophagy to facilitate viral RNA replication and virions release in epithelioma papulosum cyprinid (EPC) cells [11]. Snakehead fish vesiculovirus (SHVV) induced apparent autophagy in SSN-1 cells and autophagy inhibited virus replication [12]. Autophagy can be repressed by PI3K/Akt/mTOR pathway in tumor cells [13].…”

Section: Introductionmentioning

confidence: 99%

Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/Akt-mTOR pathway in CPB cells

Ming

et al. 2020

Fish & Shellfish Immunology

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Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre -including this research content -immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

show abstract

Autophagy induced by snakehead fish vesiculovirus inhibited its replication in SSN-1 cell line

Cited by 26 publications

References 35 publications

Potential Role of Rainbow Trout Erythrocytes as Mediators in the Immune Response Induced by a DNA Vaccine in Fish

Potential Role of Rainbow Trout Erythrocytes as Mediators in the Immune Response Induced by a DNA Vaccine in Fish

Singapore Grouper Iridovirus (SGIV) Inhibited Autophagy for Efficient Viral Replication

Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/Akt-mTOR pathway in CPB cells

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